A5006600244- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- 3D Printing in Biomedical Research
- Gene Regulatory Network Analysis
- Single-cell and spatial transcriptomics
- Chromatin Remodeling and Cancer
- Cancer-related Molecular Pathways
- Tissue Engineering and Regenerative Medicine
- Animal Genetics and Reproduction
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- Cell Image Analysis Techniques
École Polytechnique Fédérale de Lausanne
2022
Wellcome Trust
2012-2018
The Gurdon Institute
2012-2018
University of Cambridge
2012-2014
Medical Research Council
2013
Max Perutz Labs
2005-2009
Medical University of Vienna
2005-2009
Vienna Biocenter
2003-2005
University of Vienna
2002-2003
Cell populations can be strikingly heterogeneous, composed of multiple cellular states, each exhibiting stochastic noise in its gene expression. A major challenge is to disentangle these two types variability and understand the dynamic processes mechanisms that control them. Embryonic stem cells (ESCs) provide an ideal model system address this issue because they exhibit heterogeneous expression functionally important regulatory factors. We analyzed individual ESCs using single-molecule...
Histone deacetylases (HDACs) catalyze the removal of acetyl groups from core histones.Because their capacity to induce local condensation chromatin, HDACs are generally considered repressors transcription.In this report, we analyzed role class I histone deacetylase HDAC1 as a transcriptional regulator by comparing expression profiles wild-type and HDAC1-deficient embryonic stem cells.A specific subset mouse genes (7%) was deregulated in absence HDAC1.We identified several putative tumor...
The cyclin-dependent kinase inhibitor p21/WAF1/CIP1 is an important regulator of cell cycle progression, senescence, and differentiation. Genotoxic stress leads to activation the tumor suppressor p53 subsequently induction p21 expression. Here we show that cooperates with transcription factor Sp1 in promoter, whereas histone deacetylase 1 (HDAC1) counteracts p53-induced from gene. protein binds directly C terminus Sp1, a domain which was previously shown be required for interaction HDAC1....
Primordial germ cells (PGCs) and somatic originate from postimplantation epiblast in mice. As pluripotency is lost upon differentiation of lineages, a naive epigenome the network are re‐established during PGC development. Here we demonstrate that Prdm14 contributes not only to specification, but also embryonic stem (ES) by repressing DNA methylation machinery fibroblast growth factor (FGF) signalling. This indicates critical role for programming PGCs promoting ES cells.
Histone deacetylases (HDACs) are chromatin-modifying enzymes that involved in the regulation of proliferation, differentiation and development. HDAC inhibitors induce cell cycle arrest, differentiation, or apoptosis tumor cells therefore promising antitumor agents. Numerous genes were found to be deregulated upon inhibitor treatment; however, relevant target still unidentified. HDAC1 is required for mouse development unrestricted proliferation embryonic stem cells. We show here reversibly...
Article26 September 2018Open Access Transparent process Metabolic regulation of pluripotency and germ cell fate through α-ketoglutarate Julia Tischler orcid.org/0000-0003-4665-4141 Wellcome Trust/Cancer Research UK Gurdon Institute, University Cambridge, Search for more papers by this author Wolfram H Gruhn John Reid MRC Biostatistics Unit, Cambridge Institute Public Health, Biomedical Campus, The Alan Turing British Library, London, Edward Allgeyer orcid.org/0000-0002-2187-4423 Florian...
We present a low-cost, do-it-yourself system for complex mammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue plates with simple microfluidic control and automation. demonstrate the generation of concentration profiles, enabling investigation sophisticated input-response relations. further apply our automated cell-culturing platform to dynamic stimulation two widely employed stem-cell-based