A5005584788- Tuberous Sclerosis Complex Research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Renal and related cancers
- Monoclonal and Polyclonal Antibodies Research
- Treatment of Major Depression
- Biological Research and Disease Studies
- Tryptophan and brain disorders
- Platelet Disorders and Treatments
- Polyomavirus and related diseases
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Biosimilars and Bioanalytical Methods
- Animal Genetics and Reproduction
- Histiocytic Disorders and Treatments
- Computational Drug Discovery Methods
Roche (Switzerland)
2013-2019
The Gurdon Institute
2012-2014
University of Cambridge
2012-2014
Wellcome Trust
2012-2014
Medical Research Council
2013
The transcription factor Oct4 is required in vitro for establishment and maintenance of embryonic stem cells reprogramming somatic to pluripotency. In vivo, it prevents the ectopic differentiation early embryos into trophoblast. Here, we further explore role blastocyst formation specification epiblast versus primitive endoderm lineages using conditional genetic deletion. Experiments involving mouse deficient both maternal zygotic suggest that dispensable zygote formation, cleavage activation...
Primordial germ cells (PGCs) and somatic originate from postimplantation epiblast in mice. As pluripotency is lost upon differentiation of lineages, a naive epigenome the network are re‐established during PGC development. Here we demonstrate that Prdm14 contributes not only to specification, but also embryonic stem (ES) by repressing DNA methylation machinery fibroblast growth factor (FGF) signalling. This indicates critical role for programming PGCs promoting ES cells.
Epigenetic reprogramming in early germ cells is critical toward the establishment of totipotency, but investigations germline events are intractable. An objective cell culture-based system could provide mechanistic insight on how key determinants primordial (PGCs), including Prdm14, induce to an epigenetic ground state. Here we show a Prdm14-Klf2 synergistic effect that can accelerate and enhance reversion mouse epiblast stem (epiSCs) naive pluripotent state, X reactivation DNA...
Tuberous sclerosis complex (TSC) is a genetic disease characterized by benign tumor growths in multiple organs and neurological symptoms induced mTOR hyperfunction. Because the molecular pathology highly etiology poorly understood, we employed defined human neuronal model with single activating mutation to dissect disease-relevant responses driving neuropathology suggest new targets for treatment.We investigate phenotype of TSC neural differentiation stem cell that had been deleted TSC2...
Safety and efficacy of therapeutic antibodies are often dependent on their interaction with Fc receptors for IgG (FcγRs). The Göttingen minipig represents a valuable species biomedical research but its use in preclinical studies is hampered by the lack knowledge about porcine FcγRs. Genome analysis sequencing now enabled localization previously described FcγRIIIa orthologous location to human FCGR3A. In addition, we identified nearby gene coding hitherto undescribed putative FcγRIIa. 1'241...
Abstract Low-dose ketamine is an efficacious antidepressant for treatment-resistant unipolar and bipolar depressed patients. Major Depression Disorder patients receiving a single infusion report elevated mood within two hours, ketamine’s effects have been observed as long seven days post-treatment. In light of this remarkable observation, efforts undertaken to “reverse-translate” understand its mechanism action. advances achieved in understanding the molecular, cellular, circuit level...