A5025555579- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- melanin and skin pigmentation
- T-cell and B-cell Immunology
- Renal and related cancers
- Cell Adhesion Molecules Research
- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Chronic Lymphocytic Leukemia Research
- Melanoma and MAPK Pathways
- Chromosomal and Genetic Variations
- Cellular Mechanics and Interactions
- RNA regulation and disease
- Lymphoma Diagnosis and Treatment
- Biochemical Analysis and Sensing Techniques
- Wheat and Barley Genetics and Pathology
- Transgenic Plants and Applications
- Neuroscience of respiration and sleep
- Genetics and Neurodevelopmental Disorders
- Skin Protection and Aging
- Plant Reproductive Biology
- Neonatal and fetal brain pathology
University of Iceland
2013-2024
Google (United States)
2014
The Gurdon Institute
2009-2013
Wellcome Trust
2012-2013
Medical Research Council
2013
University of Cambridge
2009-2013
Wellcome/MRC Cambridge Stem Cell Institute
2013
Inner Mongolia University
2013
Columbia University
2006-2009
The cornified layer is a compacted lattice of lipid-embedded corneocytes that provides an organism's barrier to the external environment. Cornification final differentiative step for epidermal keratinocytes and involves dramatic cell condensation before death. Using conditional gene deletion in mice, we identified transcriptional repressor Blimp-1 (B lymphocyte-induced maturation protein-1) as important regulator keratinocyte transition from granular layer. More than 250 genes are...
Mice with a T cell–specific deletion of Prdm1, encoding Blimp-1, have aberrant cell homeostasis and develop fatal colitis. In this study, we show that one critical activity Blimp-1 in cells is to repress IL-2, it does so by direct repression Il2 transcription, also Fos transcription. Using these mechanisms participates an autoregulatory loop which IL-2 induces Prdm1 expression thus represses its own after activation, ensuring the immune response appropriately controlled. This important for...
Primordial germ cells (PGCs) and somatic originate from postimplantation epiblast in mice. As pluripotency is lost upon differentiation of lineages, a naive epigenome the network are re‐established during PGC development. Here we demonstrate that Prdm14 contributes not only to specification, but also embryonic stem (ES) by repressing DNA methylation machinery fibroblast growth factor (FGF) signalling. This indicates critical role for programming PGCs promoting ES cells.
Epigenetic reprogramming in early germ cells is critical toward the establishment of totipotency, but investigations germline events are intractable. An objective cell culture-based system could provide mechanistic insight on how key determinants primordial (PGCs), including Prdm14, induce to an epigenetic ground state. Here we show a Prdm14-Klf2 synergistic effect that can accelerate and enhance reversion mouse epiblast stem (epiSCs) naive pluripotent state, X reactivation DNA...
The microphthalmia-associated transcription factor (MITF) is a critical regulator of melanocyte development and differentiation. It also plays an important role in melanoma where it has been described as molecular rheostat that, depending on activity levels, allows reversible switching between different cellular states. Here, we show that MITF directly represses the expression genes associated with extracellular matrix (ECM) focal adhesion pathways human cells well regulators...
T cell-specific deletion of Blimp-1 causes abnormal cell homeostasis and function, leading to spontaneous, fatal colitis in mice. Herein we explore the role Th1/Th2 differentiation. mRNA protein are more highly expressed Th2 cells compared with Th1 cells, attenuates IFN-gamma production CD4 activated under nonpolarizing conditions. Although Blimp-1-deficient differentiate normally cytokines vitro, is required vivo for normal humoral responses NP-KLH (4-hydroxy-3-nitrophenylacetyl/keyhole...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 491:277-293 (2013) - DOI: https://doi.org/10.3354/meps10455 Influence of wintering area on persistent organic pollutants in a breeding migratory seabird Eliza H. K. Leat1,*, Sophie Bourgeon2, Ellen Magnusdottir3, Geir W. Gabrielsen4, James Grecian1, Sveinn A. Hanssen2, Kristin Olafsdottir5, Aevar Petersen6,...
The zinc-finger PR domain transcriptional repressor Blimp-1/Prdm1 plays essential roles in primordial germ cell specification, placental, heart, and forelimb development, plasma differentiation, T-cell homeostasis. present experiments demonstrate that the mouse Prdm1 gene has three alternative promoter regions. All first exons splice directly to exon 3, containing translational start codon. To examine possible cell-type-specific functional activities vivo, we generated targeted deletions...
The mild hypothermia response (MHR) maintains organismal homeostasis during cold exposure and is thought to be critical for the neuroprotection documented with therapeutic hypothermia. To date, little known about transcriptional regulation of MHR. We utilize a forward CRISPR-Cas9 mutagenesis screen identify histone lysine methyltransferase SMYD5 as regulator represses key MHR gene SP1 at euthermia. This repression correlates temperature-dependent levels H3 26 trimethylation (H3K36me3) locus...
ABSTRACT Despite the extensive erasure of DNA methylation in early human germline, nearly eight percent CpGs are resistant to epigenetic resetting acutely hypomethylated primordial germ cells (week 7-9 hPGCs). Whether this occurs stochastically or represents relatively conserved layer information is unclear. Here we show that several predominantly hominoid-specific families transposable elements (TEs) consistently resist demethylation (henceforth called hPGC-methylated TEs ‘escapees’) during...
Introduction The microphthalmia transcription factor Mitf has been shown to regulate B cell activation and tolerance. However, the underlying cell-specific mechanisms responsible, those that distinguish from closely related Mitf/TFE (MiT) factors Tfe3, Tfeb, Tfec, remain obscure. Methods Two complementary mouse models of MiT deficiency were used: mi-vga9/mi-vga9 systemic loss-of-function mutation, B-cell specific family inactivation via transgenic expression a trans-dominant negative (TDN)...
Abstract The microphthalmia associated transcription factor (MITF) is a critical regulator of melanocyte development and differentiation. It also plays an important role in melanoma where it has been described as molecular rheostat that, depending on activity levels, allows reversible switching between different cellular states. Here we show that MITF directly represses the expression genes with extracellular matrix (ECM) focal adhesion pathways human cells well regulators epithelial to...
The mild hypothermia response (MHR) maintains organismal homeostasis during cold exposure and is thought to be critical for the neuroprotection documented with therapeutic hypothermia. To date, little known about transcriptional regulation of MHR. We utilize a forward CRISPR-Cas9 mutagenesis screen identify histone lysine methyltransferase SMYD5 as regulator represses key MHR gene