A5086467183- melanin and skin pigmentation
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Wnt/β-catenin signaling in development and cancer
- Melanoma and MAPK Pathways
- RNA regulation and disease
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Skin Protection and Aging
- Cancer-related gene regulation
- Hedgehog Signaling Pathway Studies
- Developmental Biology and Gene Regulation
- PI3K/AKT/mTOR signaling in cancer
- Hair Growth and Disorders
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
- thermodynamics and calorimetric analyses
- Skin and Cellular Biology Research
- Lipid Membrane Structure and Behavior
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- 14-3-3 protein interactions
Inserm
2016-2025
Université Paris-Saclay
2016-2025
Hypertension pulmonaire : physiopathologie et innovation thérapeutique
2025
Centre National de la Recherche Scientifique
2014-2024
Université Paris Sciences et Lettres
2016-2024
Institut Curie
2015-2024
Université Paris-Sud
2016-2023
La Ligue Contre le Cancer
2014-2021
Biologie cellulaire et Cancer
1999-2015
University of Miami
2015
Identification of signaling molecules that regulate cell migration is important for understanding fundamental processes in development and the origin various pathological conditions. The Nara Bladder Tumor II (NBT-II) cells was used to determine which are specifically involved collagen-mediated locomotion. We show here paxillin tyrosine phosphorylated after induction motility on collagen. Overexpression mutants 31 and/or 118 were replaced by phenylalanine effectively impaired motility....
Molecular signatures specific to particular tumor types are required design treatments for resistant tumors. However, it remains unclear whether tumors and corresponding cell lines used drug development share such signatures. We developed similarity core analysis (SCA), a universal unsupervised computational framework extracting molecular features common lines. applied SCA mRNA/miRNA expression data from various sources, comparing melanoma metastases. The signature obtained was associated...
Cooling populations of newly formed mouse human heterokaryons has effects on the intermixing and surface antigens which indicate occurrence phase separations in membrane lipids. Antigen mixing, previously shown to be due diffusion plane membrane, is retarded when cells are cooled from 37° 21°C, but then speeded by further cooling 15°C. This result accord with observations lipids artificial bacterial membranes.
BackgroundFermitin family member 1 (FERMT1, Kindlin-1) is an epithelial-specific regulator of integrin functions and associated with Kindler syndrome, a genetic disorder characterized by skin blistering, atrophy, photosensitivity. However, the possible role kindlin-1 in cancer remains unknown.
Cell migration is a key biological process with role in both physiological and pathological conditions. Locomotion of cells during embryonic development essential for their correct positioning the organism; immune have to migrate circulate response injury. Failure or an inappropriate acquisition migratory capacities can result severe defects such as altered pigmentation, skull limb abnormalities development, defective wound repair, immunosuppression tumor dissemination. The ability...
The epithelial to mesenchymal transition (EMT) is an essential process during development and tumor progression. Here, we observed the accumulation of selective autophagy receptor signaling adaptor sequestosome-1 (SQSTM1/p62) growth factor-induced EMT in immortalized tumor-derived cell lines. Modulation p62 level regulated expression junctional proteins. This effect was dependent on ubiquitin-associated domain p62, which stabilized TGFβ/Smad co-activator Smad4 transcription factor Twist....
Abstract Loss of the tumour suppressor PTEN is frequent in human melanoma, results MAPK activation, suppresses senescence and mediates metastatic behaviour. How loss these effects unknown. Here we show that epithelial melanocytic cell lines induces nuclear localization transcriptional activation β-catenin independent PI3K–AKT–GSK3β axis. The absence leads to caveolin-1 (CAV1)-dependent modulation vitro , cooperates with NRAS Q61K initiate melanomagenesis vivo efficient metastasis formation...
Basal-like breast cancer is a heterogeneous disease characterized by the expression of basal cell markers, no estrogen or progesterone receptor and lack HER2 overexpression. Recent studies have linked activation Wnt/β-catenin pathway, its downstream target, Myc, to basal-like cancer. Transgenic mice K5ΔNβcat previously generated our team present constitutive signaling in myoepithelial layer, resulting focal mammary hyperplasias that progress invasive carcinomas. Mammary lesions developed...
The mammary epithelium comprises two major cell lineages: basal and luminal. Basal cells (BCs) isolated from the transplanted into mouse fat pad cleared endogenous regenerate gland, strongly suggesting that epithelial compartment harbors a long-lived population with multipotent stem potential. luminal layer is devoid of regenerative potential, but it contains clonogenic capacity, progenitors. Mammary BCs progenitors express high levels transcription factor Myc. Here, we show deletion Myc led...
Mammary epithelium comprises a layer of luminal cells and basal myoepithelial cell layer. Both mammary epithelial compartments, luminal, contain stem progenitor cells, but only are capable gland regeneration upon transplantation. Aberrant expansion stem/progenitor populations is considered to contribute breast tumorigenesis. Germline deletions p53 in humans mice confer predisposition tumors, frequency abnormally high the p53-deficient mice. However, it unknown whether amplification occurs...
Abstract RAS is frequently mutated in various tumors and known to be difficult target. NRAS Q61K/R are the second most frequent mutations found human skin melanoma after BRAF V600E . Aside from surgery, approaches, including targeted therapies, immunotherapies, combination used treat patients carrying mutations, but they inefficient. Here, we established mouse Q61K cell lines genetically derived isografts (GDIs) Tyr::NRAS that can vitro vivo an immune‐competent environment (C57BL/6) test...
Abstract Although numerous epigenetic aberrancies accumulate in melanoma, their contribution to initiation and progression remain unclear. The mark 5-hydroxymethylcytosine (5hmC), generated through TET-mediated DNA modification, is now referred as the sixth base of has recently been reported a potential biomarker for multiple types cancer. Loss 5hmC an hallmark but whether decrease 5hmc levels contributes directly pathogenesis or it merely results from disease progression–associated...
HGF/Met signaling has recently been associated with basal-type breast cancers, which are thought to originate from progenitor cells residing in the luminal compartment of mammary epithelium. We found that ICAM-1 efficiently marks progenitors comprising hormone receptor-positive and receptor-negative cells, presumably ductal alveolar progenitors. Both cell populations strongly express Met, while HGF is produced by stromal basal myoepithelial cells. show persistent treatment stimulates...
Fibroblast growth factor (FGF) receptor (FGFR) signaling controls the migration of glial, mesodermal, and tracheal cells in Drosophila melanogaster. Little is known about molecular events linking activation to cytoskeletal rearrangements during cell migration. We have performed a functional characterization Downstream-of-FGFR (Dof), putative adapter protein that acts specifically FGFR signal transduction Drosophila. By combining reverse genetic, culture, biochemical approaches, we...
This chapter contains section titled: Diffusion of Surface Antigens in Heterokaryons Changes Lipid Order Correlated with Antigen Rate Acknowledgements References Discussion