A5008823637- melanin and skin pigmentation
- Skin Protection and Aging
- Dermatologic Treatments and Research
- Systemic Sclerosis and Related Diseases
- RNA regulation and disease
- Mast cells and histamine
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
- Advancements in Transdermal Drug Delivery
- Connective Tissue Growth Factor Research
- Herpesvirus Infections and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Porphyrin Metabolism and Disorders
- CRISPR and Genetic Engineering
- Biochemical Analysis and Sensing Techniques
- Hair Growth and Disorders
- Virus-based gene therapy research
- Dermatology and Skin Diseases
- Cutaneous Melanoma Detection and Management
- Olfactory and Sensory Function Studies
- Vascular Malformations and Hemangiomas
- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
Inserm
2016-2025
Bordeaux Population Health
2015-2025
Université de Bordeaux
2016-2025
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2011-2024
Hôpital Saint-André
2011-2023
Centre Hospitalier Universitaire de Bordeaux
2008-2022
Université de Strasbourg
2022
Skin Research Center
2019
Tel Aviv Sourasky Medical Center
2017
Hôpital Pellegrin
2002-2014
In vitiligo, melanocytes are gradually lost in depigmented macules of the skin. The disappearance has, however, not been clearly observed and consequently aetiology disease (autoimmune, neural, cytotoxic) is still elusive. starting point vitiligo frequently determined by local conditions such as wounds excoriations, but may also follow minor traumas pressure or repeated friction. This prominent feature often neglected.To clarify biological consequences friction on attachment survival non...
UV-induced apoptosis in keratinocytes is a highly complex process which various molecular pathways are involved. These include the extrinsic pathway via triggering of death receptors and intrinsic DNA damage reactive oxygen species (ROS) formation. In this study we investigated effect catalase CuZn-superoxide dismutase (SOD) overexpression on induced by UVB exposure at room temperature or 4 °C normal human keratinocytes. Irradiation low reduced 40% independently any change p53 with decrease...
In mouse and human skin, HIF-1α is constitutively expressed in the epidermis, mainly basal layer. has been shown to have crucial systemic functions: regulation of kidney erythropoietin production mice with constitutive epidermal deletion, hypervascularity following overexpression. However, its local role keratinocyte physiology not clearly defined. To address function we used model knockout targeted keratinocytes (K14-Cre/Hif1a(flox/flox)). These had a delayed skin phenotype characterized by...
Journal Article Inflammasome activation and vitiligo/nonsegmental vitiligo progression Get access J. Marie, Marie National Reference Centre for Rare Skin Diseases Hôpital St André CHU de Bordeaux France Search other works by this author on: Oxford Academic Google Scholar D. Kovacs, Kovacs IRCCS San Gallicano Dermatological Institute Rome Italy C. Pain, Pain FranceUniversity 146 rue Léo Saignat 33076 FranceINSERM U1035 Biothérapie des Maladies Génétiques et Cancers T. Jouary, Jouary Cota,...
Using chimeric human epidermal reconstructs, we previously demonstrated that pigmentation is dependent upon the phototype of melanocytes. We report here several lines experimental evidence for dermal modulation pigmentation. First, II-III reconstructs grafted on back immunotolerant Swiss nu/nu mice developed a patchy presence colonizing or mouse fibroblasts. Similarly, white Caucasoid split-thickness skin xenografted same strain became black within 3 months and histochemistry revealed VI...
Journal Article Pre‐ vs. post‐pubertal onset of vitiligo: multivariate analysis indicates atopic diathesis association in pre‐pubertal vitiligo Get access K. Ezzedine, Ezzedine Department Dermatology and Paediatric National Centre for Rare Skin Disorders, CHU St‐André, 1 rue Jean Burguet, Hôpital Pellegrin, 33075 Bordeaux, FranceUniversity Bordeaux Inserm U1035, University France Khaled E‐mail: khaled.ezzedine@chu‐bordeaux.fr Search other works by this author on: Oxford Academic Google...
Abstract Melasma is a common acquired hyperpigmentary disorder occurring primarily in photo‐exposed areas and mainly affecting women of childbearing age. To decipher the role sex hormones melasma, this viewpoint reviews effects on cutaneous cells cultured monolayers, coculture, 3D models explants presence or absence UV . The data show that steroid hormones, especially oestrogen, can modulate vitro pigmentation by stimulating melanocytes keratinocyte pro‐pigmentary factors, but not via...
Systemic Sclerosis (SSc) is a complex auto-immune connective tissue disease combining inflammatory, vasculopathic and fibrotic manifestations. Skin effectively recapitulates the main pathogenic processes therefore good organ to decipher pathophysiology, which remains unclear. However, culturing primary skin cells SSc can be major issue due small sample size combined fibrosis. Here, we present protocol allowing isolate culture four types of cells: dermal (microvascular endothelial - HDMECs...
Common generalized vitiligo is an acquired depigmenting disorder characterized by a chronic and progressive loss of melanocytes from the epidermis hair follicles. We previously proposed new theory that involves detachment transepidermal caused autoimmune, neural impaired redox mechanisms associated with mechanical trauma. In this study, we reconstructed on dead de-epidermized dermis normal and/or non-segmental non-lesional (NSV) cells tested catecholamines or sera hydrogen peroxide. Under...
Although mixed forms have been described recently, segmental (SV) and nonsegmental vitiligo (NSV) are considered as clinically distinct. However, limited epidemiological data available to help distinguish associated factors, recent genome-wide association studies restricted NSV. The higher prevalence of SV in children is helpful when comparing the two major presentations disease.To compare factors with NSV, especially for markers autoimmunity or autoinflammation.We conducted a single-centre...
Background Until now, segmental vitiligo has been considered as a stable entity and mixed vitiligo, the association of nonsegmental reported rarely. Objectives The aim this study was to search for factors associated with generalization in patients vitiligo. Patients methods This prospective observational conducted clinic Department Dermatology Bordeaux, France. Vitiligo European Task Force questionnaire completed each patient attending confirmed diagnosis after exclusion other forms (focal,...
We have hypothesised that melanocytes disappear in vitiligo because they are weakly attached to the epidermal basal membrane (melanocytorrhagy). In epidermis, attachment of collagen IV is mediated through DDR1, which under control CCN3. DDR1 genetic variants been associated with patients different ethnic origin. vitro studies shown inhibition CCN3 induces detachment melanocytes. studied parallel expression and lesional perilesional skin impact silencing normal human on their behaviour...
Significance Although a role for β-adrenergic receptor (ADRB) in cancer progression was suggested long time ago, our serendipitous observation of the propranolol efficacy treating infantile hemangioma (IH) has driven attention exploring antitumor properties β-blockers. We identified Aquaporine-1 (AQP1) as player response and histological marker IH vascular structures including telocytes, which are dendritic cells that form distinctive peripheral layer IH. conclude sensitivity to may rely, at...
Background: Senile lentigo (SL) is a common component of photoaged skin. It characterized by hyperpigmented macules which affect chronically irradiated skin mostly after 50 years age. This study was undertaken to assess the basic morphology SL on dorsum hands. Methods: A systematic comparison between lesional vs. perilesional using immunohistochemistry and electron microscopy done detect precursor lesions determine whether melanocytes or keratinocytes were first affected in evolution...
The combination of skin ablation and 5-Fluorouracil (5-FU) ointment was previously tried in the treatment vitiligo, good results were specifically reported glabrous without follicular melanocyte reservoirs. Methods. This study carried out on skins seven guinea pigs: three treated with mechanical dermabrasion plus topical 5-FU an achromic area contiguous to a pigmented area; two by only similar alone. Clinical, histological, ultrastructural studies performed over months. Results. In pigs...