A5018748388- Fungal and yeast genetics research
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Mitochondrial Function and Pathology
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Advanced Proteomics Techniques and Applications
- Bacterial Genetics and Biotechnology
- Chronic Myeloid Leukemia Treatments
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- Trace Elements in Health
- Plant-Microbe Interactions and Immunity
- Prion Diseases and Protein Misfolding
- Antimicrobial Peptides and Activities
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- Polysaccharides and Plant Cell Walls
- Heavy metals in environment
- DNA Repair Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Bacterial biofilms and quorum sensing
Université de Bordeaux
2011-2021
Institut de Génomique Fonctionnelle
2011-2021
Centre National de la Recherche Scientifique
1990-2019
Inserm
2018
Institut Polytechnique de Bordeaux
2016-2018
Bordeaux Population Health
2018
Digital Proteomics (United States)
2016
Victor (Japan)
2005
Galen University
2005
Boiron (France)
2005
A large body of evidence from the past decade supports existence functional microdomains in membranes animal and yeast cells, which play important roles protein sorting, signal transduction, or infection by pathogens. They are based on dynamic clustering sphingolipids cholesterol ergosterol characterized their insolubility, at low temperature, nonionic detergents. Here we show that similar also exist plant plasma membrane isolated both tobacco leaves BY2 cells. Tobacco lipid rafts were found...
Poplar is the first forest tree genome to be decoded. As an initial step comprehensive analysis of poplar proteome, we described reference 2-D-maps for eight tissues/organs plant, and functional characterization some proteins. A total 398 proteins were excised from gels. About 91.2% identified by nanospray LC-MS/MS, based on comparison with 260 000 Populus sp. ESTs. In comparison, reliable PMFs obtained only 51% spots MALDI-TOF-MS, which 43% (83 spots) positively matched gene models...
The characterization of proteins isolated from skin tissue is apparently an essential parameter for understanding grape ripening as this contains the key compounds wine quality. It has been particularly difficult to extract skins analysis by two-dimensional electrophoresis gels and, therefore, a protocol purpose adapted. focus was on evolution proteome profile during maturation. Proteome maps obtained at three stages were compared assess extent which protein distribution differs in ripening....
Bacterial biofilms are predominant in natural ecosystems and constitute a public health threat because of their outstanding resistance to antibacterial treatments especially antibiotics. To date, several systems have been developed grow bacterial order study phenotypes the physiology sessile cells. Although relevant, such permit analysis various aspects biofilm state but often after hours growth.
Podocytes play a key role in diabetic nephropathy pathogenesis, but alteration of their metabolism remains unknown human kidney. By using conditionally differentiating podocyte cell line, we addressed the functional and molecular changes energetics during vitro development or under high glucose conditions. In 5 mM medium, observed stepwise activation oxidative differentiation that was characterized by peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α)-dependent stimulation...
Mitochondrial dysfunction in the spinal cord is a hallmark of amyotrophic lateral sclerosis (ALS), but neurometabolic alterations during early stages disease remain unknown. Here, we investigated bioenergetic and proteomic changes ALS mouse motor neurons patients' skin fibroblasts. We first observed that SODG93A mice presymptomatic display coupling efficiency oxidative phosphorylation, along with fragmentation mitochondrial network. The proteome also revealed peculiar metabolic signature...
Wood is one of our most important natural resources. Surprisingly, we know hardly anything about the details process wood formation. The aim this work was to describe main proteins expressed in forming tissue a conifer species (Pinus pinaster Ait.). Using high resolution 2-DE with linear pH gradient ranging from 4 7, total 1039 spots were detected. Out 240 analyzed by MS/MS, 67.9% identified, 16.7% presented no homology databases, and 15.4% corresponded protein mixtures. 57 MALDI-MS, only...
Study of the complexome - all protein complexes cell is essential for a better understanding and more global vision function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, cytosolic membrane Escherichia coli were separated. Then, different partners each complex identified by LC-MS/MS. In this report, 306 separated identified. Among these complexes, 50 heteromultimeric 256 homomultimeric found. 18 previously described validate technology. study, 109 new found,...
Abstract Selection of mutations, based on the suppression rvs161 Δ defects, was performed. Ten mutants were obtained, ranged amongst four complementation groups, named SUR1, SUR2, SUR3 and SUR4 . All sur mutations also suppress a mutation in another gene, RVS167 , indicating that all six genes are involved same biological pathway. The mutant cells have abnormal morphologies stationary phase, i.e. dumbbell‐like sur1, sur2 or sur3 strains multibudded sur4 strains. Several phenotypic...
Hepatocellular carcinoma (HCC) is a major complication of chronic viral hepatitis C. Therapy for HCC still disappointing. It thus great importance to identify novel markers early detection the disease, and tumor-specific proteins as potential therapeutic targets. We have used proteomic approach new involved in development. Four cases developing from C were analyzed by two-dimensional electrophoresis (2-DE), results compared those paired adjacent non-tumorous liver tissues. For MS...
Highlights•Specific metabolic modifications precede UVB-induced primary tumor formation•DHODH fuels mitochondrial respiration to coordinate DNA repair and ATP synthesis•Respiration-linked nucleotide synthesis is necessary for skin formation•Impairment of ETC blocks neoplastic transformation keratinocytesSummaryAlthough growing evidence indicates that bioenergetic metabolism plays an important role in the progression tumorigenesis, little information available on contribution reprogramming...
Abstract Climate change scenarios predict an increase in mean air temperatures and the frequency, intensity, length of extreme temperature events many wine‐growing regions worldwide. Because elevated has detrimental effects on berry growth composition, it threatens economic environmental sustainability wine production. Using Cabernet Sauvignon fruit‐bearing cuttings, we investigated high (HT) grapevine berries through a label‐free shotgun proteomic analysis coupled to complementary...
Biofilms are present in all environments and often result negative effects due to properties of the biofilm lifestyle especially antibiotics resistance. associated with chronic infections. Controlling bacterial attachment, first step formation, is crucial for fighting against subsequently preventing persistence infection. Thus deciphering underlying molecular mechanisms involved attachment could allow discovering targets from it would be possible develop inhibitors colonization potentiate...
Using Botrytis cinerea we confirmed in the present work several previous studies showing that salicylic acid, a main plant hormone, inhibits fungal growth vitro. Such an inhibitory effect was also observed for two acid derivatives, methylsalicylic and acetylsalicylic acid. In marked contrast, 5-sulfosalicylic totally inactive. Comparative proteomics from treated vs. control mycelia showed both intracellular extracellular proteomes were affected presence of or These data suggest mechanisms...
Significance Although a role for β-adrenergic receptor (ADRB) in cancer progression was suggested long time ago, our serendipitous observation of the propranolol efficacy treating infantile hemangioma (IH) has driven attention exploring antitumor properties β-blockers. We identified Aquaporine-1 (AQP1) as player response and histological marker IH vascular structures including telocytes, which are dendritic cells that form distinctive peripheral layer IH. conclude sensitivity to may rely, at...
Abstract Like seed plants, liverworts synthesize and accumulate a myriad of isoprenoid compounds. Using antibodies raised against several biosynthetic enzymes, we investigated their intracellular compartmentation by in situ immunolocalization from Marchantia polymorpha. The enzymes examined were deoxy-xylulose phosphate synthase, geranyl diphosphate farnesyl geranylgeranyl monoterpene reductase, phytoene desaturase. Our results show that liverwort oil bodies, which are organelles bound...
Although essential in many cellular processes, metals become toxic when they are present excess and constitute a global environmental hazard. To overcome this stress, fungi have evolved several mechanisms at both intracellular extracellular levels. In particular, well known for their ability to secrete large panel of proteins. However, role the adaptation metal toxicity has not yet been investigated. address question, here, fungus Botrytis cinerea was challenged copper, zinc, nickel or...
ABSTRACT In this report, we have shown that the yeast amphiphysin-like protein Rvs167p was localized mainly in small cortical patches throughout cell unbudding cells. During budding, were polarized at bud emergence site. mating, concentrated tip of shmoo. colocalized with actin during vegetative growth and mating. Complete disruption cytoskeleton using Latrunculin-A did not affect localization cell. rvs167 mutant cells, are mislocalized rvs161 or abp1 is affected. These observations suggest...
Nickel and cobalt are obligate nutrients for the gammaproteobacteria but when present at high concentrations they display toxic effects. These two metals in environment, their origin being either from natural sources or industrial use. In this study, effect of inhibitory Ni Co was assessed on soil bacterium Pseudomonas putida KT2440 using a proteomic approach. The identification more than 400 spots resulted quantification 160 proteins that underwent significant variations cells exposed to...
Carbon storage is likely to enable adaptation of trypanosomes nutritional challenges or bottlenecks during their stage development and migration in the tsetse. Lipid droplets are candidates for this function. This report shows that feeding T. brucei with oleate results a 4–5 fold increase number lipid droplets, as quantified by confocal fluorescence microscopy flow cytometry BODIPY 493/503-stained cells. The triacylglycerol (TAG) content also increased fold, labeled incorporated into TAG....