A5088903511- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant Physiology and Cultivation Studies
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Plant Virus Research Studies
- Nuts composition and effects
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Postharvest Quality and Shelf Life Management
- Plant nutrient uptake and metabolism
- Forest Insect Ecology and Management
- Plant responses to elevated CO2
- Plant Parasitism and Resistance
- Plant and Fungal Interactions Research
- Insect symbiosis and bacterial influences
- Polysaccharides and Plant Cell Walls
- Potato Plant Research
- Plant and animal studies
- Insect-Plant Interactions and Control
Centre National de la Recherche Scientifique
2009-2024
Unité de Recherche Œnologie
2017-2024
École Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine
2018-2024
Université de Bordeaux
2011-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2024
Ecophysiologie et Génomique Fonctionnelle de la Vigne
2013-2022
Institut National de la Recherche Agronomique du Niger
2012
Max Perutz Labs
2004-2011
University of Minho
2009
Université de Poitiers
2009
Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in berries response to high temperatures poorly understood. Unlike recent data that addressed effects on elevated applied at whole plant level, present work particularly focuses fruit responses triggered direct exposure heat treatment (HT). In context climate change, this focusing temperature effect microclimate level is particular interest as it...
In grape (Vitis vinifera), abscisic acid (ABA) accumulates during fruit ripening and is thought to play a pivotal role in this process, but the molecular basis of control poorly understood. This work characterizes ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 (VvABF2), basic leucine zipper transcription factor belonging phylogenetic subgroup previously shown be involved ABA abiotic stress signaling other plant species. VvABF2 transcripts mainly accumulated berry, from onset harvesting...
Among various environmental factors, temperature is a major regulator affecting plant growth, development and fruit composition. Grapevine the most cultivated throughout world, grapes are used for wine production human consumption. The molecular mechanisms involved in grapevine tolerance to high temperature, especially at level, poorly understood. To better characterize sensitivity of berries microenvironment, conditions were locally applied Vitis vinifera Cabernet Sauvignon clusters. Two...
Resveratrol (Res) is a stilbenoid, group of plant phenolic metabolites derived from stilbene that possess activities against pests, pathogens, and abiotic stresses. Only few species, including grapevine (Vitis), synthesize accumulate Res. Although synthases (STSs) have been isolated characterized in several the gene regulatory mechanisms underlying biosynthesis are still largely unknown. Here, we characterize WRKY transcription factor, VvWRKY8, regulates Res biosynthetic pathway. Transient...
Shoot branching is an important trait of plants that allows them to adapt environment changes. Strigolactones (SLs) are newly identified plant hormones inhibit shoot in plants. The SL biosynthesis genes CCD7 (carotenoid cleavage dioxygenase 7) and CCD8 have been found regulate several herbaceous by taking advantage their loss-of-function mutants. However, the role for control grapevine still unknown due lack corresponding mutants.Here we employed CRISPR/Cas9 system edit VvCCD7 VvCCD8 grape...
The development of fleshy fruits involves complex physiological and biochemical changes. After fertilization, fruit growth usually begins with cell division, continues both division expansion, allowing set to occur, ends expansion only. In spite the economical importance grapevine, molecular mechanisms controlling berry are not fully understood. present work identified characterized Vitis vinifera elongation bHLH protein (VvCEB1), a basic helix–loop–helix (bHLH) transcription factor in...
Abstract Climate change scenarios predict an increase in mean air temperatures and the frequency, intensity, length of extreme temperature events many wine‐growing regions worldwide. Because elevated has detrimental effects on berry growth composition, it threatens economic environmental sustainability wine production. Using Cabernet Sauvignon fruit‐bearing cuttings, we investigated high (HT) grapevine berries through a label‐free shotgun proteomic analysis coupled to complementary...
In grapevine (Vitis vinifera), as in many crops, soluble sugar content is a major component of yield and economical value. This paper identifies characterizes Glycogen Synthase Kinase3 protein kinase, cloned from cDNA library grape Cabernet Sauvignon berries harvested at the ripening stage. gene, called VvSK1, was mainly expressed flowers, berries, roots. it strongly postveݩraison, when accumulate glucose, fructose, abscisic acid. cell suspensions, VvSK1 transcript abundance increased by...
In Arabidopsis thaliana, the serine/threonine protein kinase oxidative signal-inducible 1 (OXI1), mediates stress signalling. Its activity is required for full activation of mitogen-activated kinases (MAPKs), MPK3 and MPK6, in response to stress. addition, Pto-interacting 1-2 (PTI1-2) has been positioned downstream from OXI1, but whether PTI1-2 signals through MAPK cascades unclear. Using a yeast two-hybrid screen we show that OXI1 also interacts with PTI1-4. PTI1-4 are stress-responsive...
Aim: In the long term, genetic improvement is one of major strategies to support sustainable wine production in a changing climate. Over past 5 years, we have developed an interdisciplinary research program that aimed to: i) characterize impact temperature increase sensed by entire plant or individual bunches on development and functioning plant, ii) identify physiological molecular mechanisms regulating response vegetative reproductive heat stress iii) develop tools map quantitative trait...
Cold tolerance is regulated by a variety of transcription factors (TFs) and their target genes. Except for the well-characterized C-repeat binding (CBFs)-dependent transcriptional cascade, mechanisms cold mediated other regulatory networks are still largely unknown. Here, we used assay transposase-accessible chromatin with sequencing (ATAC-seq) RNA-seq to identify responsive TFs in Vitis amurensis, grape species high hardiness. Nine TFs, including CBF4, RAV1 ERF104, were identified after...
Abstract Background For ten years, CRISPR/cas9 system has become a very useful tool for obtaining site-specific mutations on targeted genes in many plant organisms. This technology opens up wide range of possibilities improved breeding the future. In plants, CRISPR/Cas9 is mostly used through stable transformation with constructs that allow expression Cas9 gene and sgRNA. Numerous studies have shown mutation efficiency can vary greatly between different species due to factors such as...
We previously reported that the signal transduction of cryptogein, an elicitor defense reactions in Nicotiana tabacum cells, involves upstream protein phosphorylation. In present study, induction these early physiological events was further investigated with inhibitors phosphatase (PP), okadaïc acid, and calyculin A. Calyculin A mimicked effects inducing influx calcium, extracellular alkalinization, production active oxygen species (AOS), suggesting during cryptogein balance between specific...
Context of the review: The manipulation genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies. In last 15 years, considerable knowledge has accumulated about genome as well mechanisms involved in interaction vine with environment, pests diseases. Despite difficulties associated mapping this species (allele diversity, chimerism, long generation intervals...), several major important vegetative reproductive...
As the climate changes, genetic adaptation of crops to abiotic stresses is an increasingly important issue, especially for a perennial high economic value such as grapevine. Given numerous environments where this plant can be found, and huge intra- interspecific diversity, we assume that grapevine genomes contain many alleles could exploited ensure sustainability viticulture. The challenge identify these understand how they used manipulate phenotypes. diversity constraints (thermal stress,...
Abstract Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola , but associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses grapevine leaves from resistant genotype V. davidii “LiuBa-8” (LB) and susceptible vinifera “Pinot Noir” (PN) 12 h after inoculation with . By employing iTRAQ technique, a total 444 349 differentially expressed proteins (DEPs) were identified in LB PN,...
During grape berry ripening, the vacuoles accumulate water, sugars and secondary metabolites, causing great impact in plant productivity wine quality. However, molecular basis of these compartmentation processes is still poorly understood. As many species, major bottleneck to study aspects grapevine obtain highly purified with a good yield. The present paper describes an isolation method protoplasts intact from cells their functional characterization by transport cytometric assays....
Through its role in the regulation of gene expression, DNA methylation can participate control specialized metabolite production. We have investigated link between and anthocyanin accumulation grapevine using hypomethylating drug, zebularine Gamay Teinturier cell suspensions. In this model, increased light, induced production dark. To unravel underlying mechanisms, transcriptome, metabolic content, were analyzed. The up-regulation stress-related genes, as well a decrease viability, revealed...
Abstract CRISPR-based genome editing systems have been successfully and effectively used in many organisms. However, only a few studies reported the comparison between CRISPR/Cas9 CRISPR/Cpf1 whole-genome applications. Although web-based toolkits are available, there is still shortage of comprehensive, user-friendly, plant-specific CRISPR databases desktop software. In this study, we identified analyzed similarities differences by considering abundance proto-spacer adjacent motif (PAM)...