A5041273657- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Cell Adhesion Molecules Research
- Fungal Plant Pathogen Control
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Bone and Dental Protein Studies
- Plant Disease Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant Pathogens and Fungal Diseases
- Mosquito-borne diseases and control
- Protease and Inhibitor Mechanisms
- Collagen: Extraction and Characterization
- Antimicrobial Peptides and Activities
- Oral and Maxillofacial Pathology
- Chromium effects and bioremediation
- Vector-borne infectious diseases
- Biochemical and Structural Characterization
- Connective Tissue Growth Factor Research
- Signaling Pathways in Disease
- Skin Protection and Aging
- Oral microbiology and periodontitis research
- Receptor Mechanisms and Signaling
- Photodynamic Therapy Research Studies
- Forest Ecology and Biodiversity Studies
Université Claude Bernard Lyon 1
2012-2025
Centre National de la Recherche Scientifique
2012-2025
Biologie Tissulaire et Ingénierie Thérapeutique
2020-2024
Institut National des Sciences Appliquées de Lyon
2023
Inserm
2020
École Normale Supérieure de Lyon
2020
Structure Fédérative de Recherche Biosciences
2020
Laboratoire de Microbiologie, Adaptation et Pathogénie
2019
Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères
2017-2019
Bayer (France)
2018
Summary Extracellular proteins play crucial roles in the interaction between mycorrhizal fungi and their environment. Computational prediction experimental detection allowed identification of 869 constituting exoproteome Hebeloma cylindrosporum . Small secreted ( SSP s) carbohydrate‐active enzymes CAZ ymes) were two major classes extracellular proteins. Twenty‐eight per cent s by free‐living mycelia five 10 most abundant s. By contrast, 63–75% involved nutrient acquisition secreted. A total...
Summary The phytopathogenic fungus Botrytis cinerea is able to infect a wide variety of plants and plant tissues with differing chemical compositions. During its interaction the host, this pathogen modulates ambient pH by secreting acids or ammonia. In work, we examined Pal/Pac pathway, fungal pH‐responsive signalling circuit, investigated role PacC transcription factor. Characterization BcpacC deletion mutant revealed an alteration both growth virulence depending on culture medium host...
Using Botrytis cinerea we confirmed in the present work several previous studies showing that salicylic acid, a main plant hormone, inhibits fungal growth vitro. Such an inhibitory effect was also observed for two acid derivatives, methylsalicylic and acetylsalicylic acid. In marked contrast, 5-sulfosalicylic totally inactive. Comparative proteomics from treated vs. control mycelia showed both intracellular extracellular proteomes were affected presence of or These data suggest mechanisms...
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Although essential in many cellular processes, metals become toxic when they are present excess and constitute a global environmental hazard. To overcome this stress, fungi have evolved several mechanisms at both intracellular extracellular levels. In particular, well known for their ability to secrete large panel of proteins. However, role the adaptation metal toxicity has not yet been investigated. address question, here, fungus Botrytis cinerea was challenged copper, zinc, nickel or...
Fungal secretomes are known to contain a multitude of components involved in nutrition, cell growth or biotic interactions. Recently, extra-cellular vesicles have been identified few fungal species. Here, we used multidisciplinary approach identify and characterize extracellular produced by the plant necrotroph Botrytis cinerea. Transmission electron microscopy infectious hyphae grown vitro revealed various sizes densities. Electron tomography showed co-existence ovoid tubular pointed their...
Abstract G‐Protein Coupled Receptor, Class C, Group 5, Member A (GPRC5A) has been extensively studied in lung and various epithelial cancers. Nevertheless, its role the skin remains to be elucidated. In this study, we sought investigate function of receptor biology. Our research demonstrated that expression responds mechanical substrate changes human primary keratinocytes. Furthermore, observed reinduction GPRC5A during wound healing at leading edges an ex vivo burn model, coinciding with...
The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order decipher its infectious strategy, library 2,144 mutants was generated by random insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation (ATMT). Twelve exhibiting total loss virulence toward different plants were chosen for detailed analyses. Their molecular characterization revealed...
Aedes albopictus is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The competence Ae. varies depending on the mosquito population involved virus transmitted. Wolbachia infection status believed to be among key elements determine viral transmission efficiency. Little known about cellular functions mobilized during co-infection by given arbovirus. To decipher this tripartite interaction at molecular level, we performed proteome...
Fungi are the most prevalent plant pathogens, causing annually important damages. To infect and colonize their hosts, they secrete effectors including hydrolytic enzymes able to kill macerate tissues. These secreted proteins transported from Endoplasmic Reticulum Golgi apparatus extracellular space through intracellular vesicles. In pathogenic fungi, vesicles were described but biogenesis role in virulence remain unclear. this study, we report essential of clathrin heavy chain (CHC)...
BMP-1/tolloid-like proteinases (BTPs) are major players in tissue morphogenesis, growth and repair. They act by promoting the deposition of structural extracellular matrix proteins controlling activity matricellular TGF-β superfamily factors. have also been implicated several pathological conditions such as fibrosis, cancer, metabolic disorders bone diseases. Despite this broad range pathophysiological functions, putative existence a specific endogenous inhibitor capable their activities...
Abstract The proteolytic conversion of soluble procollagens into mature collagen monomers is a critical step to decrease their solubility and trigger fibril formation. In the case collagens I, II III, this maturation process driven by several extracellular metalloproteinases such as BMP-1, tolloid-like proteinases, meprin α, β, ADAMTS-2 ADAMTS-14 but extensive characterization these events has been hampered lack recombinant procollagens. We previously reported production partial homotrimeric...
ABSTRACT G-Protein Coupled Receptor, Class C, Group 5, Member A (GPRC5A) is well-documented in lung and various epithelial cancers. However, its role the skin remains unexplored. In this study, we investigated function of receptor biology our research demonstrated that expression responds to mechanical substrate changes human primary keratinocytes. Furthermore, observed GPRC5A reinduction during wound healing at leading edges an ex vivo burn model, coinciding with translocation C-terminal...
ABSTRACT Extracellular matrix (ECM) remodeling by proteases results in the release of protein fragments that promote tumor progression and metastasis. The protease cathepsin D (cath-D), a marker poor prognosis triple-negative breast cancer (TNBC), is aberrantly secreted microenvironment. Using degradomic analyses TAILS, we discovered matricellular SPARC substrate extracellular cath-D. In vitro , cath-D induced limited proteolysis C-terminal Ca 2+ binding domain at acidic pH, leading to...