A5067019796- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Mitochondrial Function and Pathology
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Hepatitis C virus research
- Hepatitis B Virus Studies
- ATP Synthase and ATPases Research
- Enterobacteriaceae and Cronobacter Research
- Antibiotics Pharmacokinetics and Efficacy
- Retinal Development and Disorders
- Superconductivity in MgB2 and Alloys
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Nuclear Structure and Function
- Plant Molecular Biology Research
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Lipid metabolism and biosynthesis
- Escherichia coli research studies
- Plant biochemistry and biosynthesis
- Nitrogen and Sulfur Effects on Brassica
Centre National de la Recherche Scientifique
1989-2022
Université de Bordeaux
1998-2022
Institut Polytechnique de Bordeaux
2011-2020
Laboratoire de Microbiologie Fondamentale et Pathogénicité
2014-2017
Pontificia Universidad Católica de Chile
2010
Institut de Biochimie et Génétique Cellulaires
1989-2000
Laboratoire de Biogenèse Membranaire
1999
RNA editing of subunit 9 the wheat mitochondrial ATP synthase has been studied by cDNA and protein sequence analysis. Most clones sequenced (95%) showed that C-to-U transitions occurred at eight positions in coding region. Consequently, 5 amino acids were changed when compared with predicted from gene. Two edited codons gave no changes (silent editing). One generated a stop codon modifying arginine CGA to UGA. Thus, produced is 6 shorter than deduced genomic sequence. Minor forms partial or...
Most plant mitochondria messenger RNAs (mRNAs) undergo editing through C-to-U conversions located mainly in exon sequences. However, some RNA events are found non-coding regions at critical positions the predicted secondary and tertiary structures of introns, suggesting that could be important for splicing. Here, we studied relationships between splicing mRNA encoding ribosomal protein S10 (rps10), which has a group II intron five sites. Two them, C2 C3, to stabilize folded structure...
In this study, we characterized the molecular basis for binding of adenovirus (AdV) to cytoplasmic face nuclear pore complex (NPC), a key step during delivery viral genome into nucleus. We used RNA interference (RNAi) deplete cells either Nup214 or Nup358, two major Phe-Gly (FG) repeat nucleoporins localized on side NPC, and evaluated impact hexon AdV infection. The accumulation purified trimers partially disassembled at envelope (NE) was observed in digitonin-permeabilized absence cytosolic...
Abstract Like seed plants, liverworts synthesize and accumulate a myriad of isoprenoid compounds. Using antibodies raised against several biosynthetic enzymes, we investigated their intracellular compartmentation by in situ immunolocalization from Marchantia polymorpha. The enzymes examined were deoxy-xylulose phosphate synthase, geranyl diphosphate farnesyl geranylgeranyl monoterpene reductase, phytoene desaturase. Our results show that liverwort oil bodies, which are organelles bound...
A posttranscriptional modification (C-to-U) at specific positions of plant mitochondrial mRNA leads to changes in the amino acid sequence as well emergence novel initiation or termination sites. This phenomenon, named RNA editing, has been described for several genes from different sources. We have found recently that editing ATP synthase subunit 9 (atp9) involves eight including creation a new stop codon. In this article, we describe an vitro system devised follow wheat atp9 mRNA. Nonedited...
Multidrug-resistant strains belonging to the Enterobacter cloacae complex (ECC) group, and especially those clusters C-III, C-IV, C-VIII, have increasingly emerged as a leading cause of health care-associated infections, with colistin used one last lines treatment. However, colistin-resistant ECC emerged. The aim this study was prove that MgrB, negative regulator PhoP/PhoQ two-component regulatory system, is involved in resistance cluster formerly referred hormaechei subsp. steigerwaltii An...
Higher plant mitochondrial RNAs undergo predetermined modifications which involve differences of splicing and trimming the primary transcripts. These post‐transcriptional are specific C‐to‐U changes occurring mostly in coding regions mRNAs without changing reading frame. Editing can lead to formation or initiation stop codons. Plant proteins issued from edited more similar non‐plant homologous suggesting that this process is involved production functional polypeptides. RNA editing non‐coding...
Biopsies from patients show that hepadnaviral core proteins and capsids - collectively called are found in the nucleus cytoplasm of infected hepatocytes. In majority studies, cytoplasmic localization is related to low viraemia while nuclear associated with high viral loads. order better understand molecular interactions leading localization, we analysed transfected hepatoma cells using immune fluorescence microscopy. We observed expression protein absence other led capsids, context resulted...
Journal Article Nucleotide sequence of the F1-ATPase subunit α genes from two lines wheat Get access Dominique Bégu, Bégu Institut de Biochimie Cellulaire et Neurochimie du CNRS1 rue Camille Saint-Saëns 33077 Bordeaux Cedex, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Pierre-Vincent Graves, Graves Simon Litvak, Litvak Alejandro Araya Nucleic Acids Research, Volume 17, Issue 22, 25 November 1989, Page 9492, https://doi.org/10.1093/nar/17.22.9492...
During a two-month period (2017-2018), 336 urine samples positive for Escherichia coli were collected from Tunisian patients. Of the samples, 266 community patients and 70 hospital settings. In all, 15 ESBL producers identified (8 7, respectively) assigned to 13 pulsotypes, including four ESBL-producing E. (ESBL-E) with E1 E2 profiles (2 isolates each) The two strains as B2-ST131 subclade C2 E2, A-ST617. carrying both CTX-M-15 CTX-M-27, exhibited multireplicon IncFII/F1A/F1B same formula...
ABSTRACT CTX-M ESBLs have been increasingly reported from human enterobacteria isolates in Tunisia. NDM-1 carbapenemase was recently Tunisian Hospitals. During a 2-month period (December 2017 to January 2018), we collected 23 ESBL-producing Enterobacteriaceae (ESBL-E) urines of patients hospitalized three health care facilities and community, situated two distinct geographical regions. They were divided into 15 Escherichia coli eight Klebsiella pneumoniae . The aim this study characterize...
Mapacalcine is a small homodimeric protein of 19 kDa with 9 disulfide bridges extracted from the Cliona vastifica sponge (Red Sea). It selectively blocks calcium current insensitive to most blockers. Specific receptors for mapacalcine have been described in variety tissues such as brain, smooth muscle, liver, and kidney. Previous works achieved on hepatocytes nervous cells demonstrated that this influx triggered by an ischemia/reperfusion (I/R) shock efficiently protects death after I/R. The...