A5081826398- HIV Research and Treatment
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Gene Regulatory Network Analysis
- Hematopoietic Stem Cell Transplantation
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- Regulation of Appetite and Obesity
- CRISPR and Genetic Engineering
- Herpesvirus Infections and Treatments
- Biochemical Analysis and Sensing Techniques
- HIV/AIDS drug development and treatment
- Vector-borne infectious diseases
- IL-33, ST2, and ILC Pathways
- Advanced Biosensing Techniques and Applications
- Acute Myeloid Leukemia Research
- Scientific Computing and Data Management
- Microfluidic and Bio-sensing Technologies
- HIV-related health complications and treatments
- Viral gastroenteritis research and epidemiology
University of Cambridge
2014-2024
Institute for Medical Research
2017
German Primate Center
2007-2014
Cancer Research UK
2014
Cancer Research UK Cambridge Center
2010
Heterogeneity within the self-renewal durability of adult hematopoietic stem cells (HSCs) challenges our understanding molecular framework underlying HSC function. Gene expression studies have been hampered by presence multiple subtypes and contaminating non-HSCs in bulk populations. To gain deeper insight into gene program murine HSCs, we combined single-cell functional assays with flow cytometric index sorting assays. Through bioinformatic integration these datasets, designed an unbiased...
Toll-like receptor (TLR) ligands are being considered as adjuvants for the induction of antigen-specific immune responses, in design vaccines. Polyriboinosinic-polyribocytoidylic acid (poly I:C), a synthetic double-stranded RNA (dsRNA), is recognized by TLR3 and other intracellular receptors. Poly ICLC poly I:C analogue, which has been stabilized against serum nucleases that present plasma primates. I:C12U, another less toxic but also stable vivo than I:C, essential its recognition. To study...
Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterized simply as direct targets for leptin, recent data suggest a more complex architecture.Using single cell RNA sequencing, we have generated an atlas gene expression murine POMC neurons.Of 163 neurons, 118 expressed high levels Pomc with little/no Agrp and were considered "canonical" (P+). The other 45/163 low (A+P+). Unbiased clustering analysis P+ revealed four different classes,...
Recent advances in the cellular and molecular biology of single stem cells have uncovered significant heterogeneity functional properties cell populations. This has prompted development approaches to study isolation, often performed using multiparameter flow cytometry. However, many populations are too rare test all possible surface marker combinations, virtually nothing is known about differences associated with varying intensities such markers. Here we describe use index sorting for...
ABSTRACT Induction of long-lasting immunity against viral respiratory tract infections remains an elusive goal. Using a nonhuman primate model human syncytial virus (hRSV) infection, we compared mucosal and systemic immune responses induced by different DNA delivery approaches to novel parenteral prime-tonsillar adenoviral vector booster immunization regimen. Intramuscular (i.m.) electroporation (EP) vaccine encoding the fusion protein hRSV stronger than intradermal EP, tattoo immunization,...
Summary All the pathology and symptoms associated with malaria are caused by growth of Plasmodium parasites inside human red blood cells. This process, which in case major pathogen falciparum takes place over a 48-hour period, involves multiple tightly regulated developmental transitions. Understanding P. genes that regulate these key processes could lead to identification targets for new drugs. However, while large-scale sequencing efforts have led good understanding genome how it evolves...
Since there is still no protective HIV vaccine available, better insights into immune mechanism of persons effectively controlling replication in the absence any therapy should contribute to improve further designs. However, little known about mucosal response this small unique group patients. Using SIV-macaque-model for AIDS, we had rare opportunity analyze 14 SIV-infected rhesus macaques durably viral (controllers). We investigated virological and immunological profile blood three...
Abstract Background Due to an ever increasing shortage of rhesus macaques Indian origin (InR) that have been generally used for preclinical AIDS vaccine trials in non‐human primates, demand is rising Chinese (ChR). However, the immunogenicity candidate has not compared parallel both macaque subspecies. Methods ChR and InR were immunized with SIV/HIV DNA adenovirus their immune responses SIV HIV evaluated. Results Gag‐ Env‐specific T‐cell SIV‐specific lymphoproliferative measured...
Abstract Background Normal human breast tissues are a heterogeneous mix of epithelial and stromal subtypes in different cell states. Delineating the spectrum cellular heterogeneity will provide new insights into normal properties within tissue that might become dysregulated initial stages cancer. Investigation surface marker expression provides valuable approach to resolve complex populations. However, majority maker primary cells have not been investigated. Methods To determine differences...
Efficacy assessment of AIDS vaccines relies both on preclinically challenging immunized monkeys with simian immunodeficiency virus (SIV) or monitoring infection rates in large human trials. Although conventional parameters vaccine-induced immune responses do not completely predict outcome, existing methods for testing cellular immunity are sophisticated and difficult to establish resource-limited settings.We have used replication kinetics (VVR) ConA-stimulated peripheral blood mononuclear...
Because of their high antigen specificity and metabolic stability, genetically engineered human monoclonal antibodies are on the way to becoming one most promising medical diagnostics therapeutics. In order establish an in vitro system capable producing such biosimilar antibodies, we used constant chain sequences design novel antibody expressing vector cassette pMAB-ABX. A bidirectional tetracycline (tet)-controllable promotor was for harmonized expression immunoglobulin type G (IgG) heavy...
Summary Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterised simply as direct targets for leptin, recent data suggest a more complex architecture. Using single cell RNA sequencing, we have generated an atlas gene expression murine POMC neurons. Of 163 neurons, 118 expressed high levels Pomc with little/no Agrp and were considered “canonical” (P + ). The other 45/163 low (A P Unbiased clustering analysis revealed four different...
Biomedical specialist core facilities are major part of institutional infrastructure in academia and play important role research by creating access to well established emerging technologies being a source valuable expertise. In each particular case, the extent, range content services they provide determined technology specialism, demands users institutional/overarching policies. In 2012, two flow cytometry embarked on new journey development their community Cambridge. Both started from same...
Life is spatially partitioned within lipid membranes to allow the isolated formation of distinct molecular states inside cells and organelles. Cell fusion merger two or more form a single cell. Here we provide protocol for cell different types. Fused hybrid are enriched by flow cytometry-based sorting, followed fluorescence microscopy structure function. Fluorescently tagged proteins generated genome editing imaged fused cells, allowing cellular structures be identified based on emission...
Life is spatially partitioned within lipid membranes to allow the isolated formation of distinct molecular states inside cells and organelles. Cell fusion merger two or more form a single cell. Here we provide protocol for cell different types. Fused hybrid are enriched by flow cytometry-based sorting, followed fluorescence microscopy structure function. Fluorescently tagged proteins generated genome editing imaged fused cells, allowing cellular structures be identified based on emission...