A5056528869- Parasites and Host Interactions
- Asthma and respiratory diseases
- Pediatric health and respiratory diseases
- Immune cells in cancer
- Parasite Biology and Host Interactions
- Cancer Immunotherapy and Biomarkers
- Extracellular vesicles in disease
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phagocytosis and Immune Regulation
- Immune Response and Inflammation
- Vitamin D Research Studies
- RNA Interference and Gene Delivery
- Immune responses and vaccinations
- Neonatal Respiratory Health Research
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- Parasitic Infections and Diagnostics
- MXene and MAX Phase Materials
- Herbal Medicine Research Studies
- Parasitic infections in humans and animals
Cleveland Clinic
2023-2024
Aarhus University
2018-2023
Aarhus University Hospital
2018
Ferdowsi University of Mashhad
2015-2017
Myeloid-derived suppressor cells (MDSCs) impair antitumor immune responses. Identifying regulatory circuits during MDSC development may bring new opportunities for therapeutic interventions. We report that the V-domain of T cell activation (VISTA) functions as a key enabler differentiation. VISTA deficiency reduced STAT3 and STAT3-dependent production polyamines, which causally impaired mitochondrial respiration expansion. In both mixed bone marrow (BM) chimera mice myeloid-specific...
Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function physiology. Emerging evidence supports a role for helminth-derived EVs these processes highlights as an important participant cross-phylum communication. While mammalian EV field is guided by community-agreed framework...
Immune checkpoint blockade is a promising approach to activate antitumor immunity and improve the survival of patients with cancer. V-domain immunoglobulin suppressor T cell activation (VISTA) an immune target; however, downstream signaling mechanisms are elusive. Here, we identify leucine-rich repeats immunoglobulin-like domains 1 (LRIG1) as VISTA binding partner, which acts inhibitory receptor by engaging suppressing pathways. Mice cell-specific LRIG1 deletion developed superior responses...
There is a strong correlation between dysregulation of the gastrointestinal microbiota and development allergic diseases. The most prevalent therapies for relieving asthma symptoms are associated with serious side effects, therefore novel approaches needed. Our objective was to elucidate whether oral administration Lactobacillus rhamnosus GG (LGG) as probiotic or turmeric powder (TP) prebiotic both synbiotic mitigate inflammation including lung function, airway inflammatory cell...
Abstract Emerging evidence suggests that immune cells not only communicate with each other through cytokines, chemokines, and cell surface receptors, but also by releasing small membranous structures known as extracellular vesicles (EVs). EVs carry a variety of different molecules can be taken up recipient cells. Parasitic worms are well for their immunomodulatory properties, whether they affect responses altering EV‐driven communication between host remains unclear. Here we provide...
Enormous progress in the field of vaccine development has moved area from traditional vaccines using whole microorganisms to subunit containing only purified or modified antigenic proteins. The low immunogenic potentials compared live attenuated pathogens, have prompted research towards developing new adjuvants with ability mimic and enhance innate adaptive immune responses. Novel formulations based on discovery pattern recognition receptors (PRRs), main class immunity sensors recently...
Arginase1 (ARG1) is a metabolic enzyme that highly expressed in tumor-associated myeloid-derived suppressor cells (MDSCs) and causes the dysfunction of tumor-reactive T cells. Here, we present protocol for detecting ARG1 expression tumor MDSCs from murine model colon cancer using flow cytometry. We describe steps tissue processing, antibody staining, data acquisition. then detail procedures identifying MDSC subsets precise gating strategy. For complete details on use execution this protocol,...
Regulation of macrophage (Mɸ) function can maintain tissue homeostasis and control inflammation. Parasitic worms (helminths) are potent modulators host immune inflammatory responses. They have evolved various strategies to promote immunosuppression, including redirecting phagocytic cells toward a regulatory phenotype. Although soluble products from the whipworm Trichuris suis (TSPs) shown significant effects on Mɸ function, mechanisms underlying these modulatory still not well understood. In...
<h3>Background</h3> Mononuclear and polymorphonuclear myeloid-derived suppressor cells (M-MDSC PMN-MDSC) accumulate in many cancer types impair anti-tumor immune response reduce immunotherapy efficacy.<sup>1 2</sup> The role of checkpoint protein V-domain Suppressor T cell Activation (VISTA) the differentiation function MDSC remains incompletely understood.<sup>3–10</sup> <h3>Methods</h3> Flow analysis was applied for surface makers analysis. Gene expression determined by RT-qPCR....
Abstract Immune checkpoint receptors (ICRs) negatively regulate antitumor immune responses. While significant advances have been made in understanding the mechanisms of well-established ICRs such as PD-1 and CTLA-4, other proteins remain incompletely characterized may be targeted to synergistically boost immunity. One protein is V-domain immunoglobulin suppressor T-cell activation (VISTA), an ICR whose expression curtails function several lymphoid myeloid lineages, including tumor-reactive...
<h3>Background</h3> Immune checkpoint blockade represents a promising strategy to activate anti-tumor immunity and improve the survival of patients with cancer. Recent studies have established role 'stem-like' tumor-specific CD8<sup>+</sup> T cells that express TCF-1 respond ICI therapies by exiting quiescent states proliferative burst. On other hand, mechanisms maintain quiescence TCF-1<sup>+</sup> stem-like not been well understood may underlie clinical resistance current immunotherapies....