A5085866806- Lipid Membrane Structure and Behavior
- Surfactants and Colloidal Systems
- Proteins in Food Systems
- Polymer Surface Interaction Studies
- Electrostatics and Colloid Interactions
- Spectroscopy and Quantum Chemical Studies
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Food Chemistry and Fat Analysis
- Pickering emulsions and particle stabilization
- Dendrimers and Hyperbranched Polymers
- Force Microscopy Techniques and Applications
- Enzyme Catalysis and Immobilization
- DNA and Nucleic Acid Chemistry
- Advanced Polymer Synthesis and Characterization
- Liquid Crystal Research Advancements
- Lipid metabolism and biosynthesis
- Nanopore and Nanochannel Transport Studies
- Calcium Carbonate Crystallization and Inhibition
- Analytical Chemistry and Chromatography
- Protein purification and stability
- Bone Tissue Engineering Materials
- Protein Hydrolysis and Bioactive Peptides
- Microencapsulation and Drying Processes
- Infant Nutrition and Health
Lund University
2016-2025
Sungkyunkwan University
2023-2025
Lund Science (Sweden)
2014-2021
Leman University
2021
Ideon Science Park
1997-2019
Skåne University Hospital
2017
Getinge (Sweden)
2010-2017
Mid Sweden University
2017
Nanyang Technological University
2017
Uppsala University
2008-2010
We have measured the time-resolved adsorption kinetics of mussel adhesive protein (Mefp-1) on a nonpolar, methyl-terminated (thiolated) gold surface, using three independent techniques: quartz crystal microbalance with dissipation monitoring (QCM-D), surface plasmon resonance, and ellipsometry. The QCM-D ellipsometry data shows that, after to saturation Mefp-1, cross-linking layer NaIO4 transforms it from an extended (∼20 nm), water-rich, hydrogel-like state much thinner (∼5 compact, less...
The development of lipid nanoparticle (LNP) based therapeutics for delivery RNA has triggered the advance new strategies formulation, such as high throughput microfluidics precise mixing components into well-defined particles. In this study, we have characterised structure LNPs throughout formulation process using in situ small angle x-ray scattering microfluidic chip, then by sampling subsequent dialysis process. final was investigated with (SAXS) and neutron (SANS) scattering, dynamic...
This study reveals specific ion effects on particle formation during peristaltic pumping of a monoclonal Antibody (Antibody A). For this purpose, three anions in the direct Hofmeister series were selected, ranging from kosmotropic SO42- to more neutral Cl- and chaotropic SCN-. Protein is described primarily as surface-driven mechanism. Therefore, effect was hypothesised affect with smallest amount protein adsorbing least particles formed presence SCN-, followed by highest SO42-. The...
The phase behavior and microstructure of the two ternary systems monoolein (MO)−sodium oleate (NaO)−water (2H2O) MO−oleic acid (OA)−2H2O are studied by a combination optical microscopy, cryo-transmission electron small-angle X-ray diffraction, NMR methods. results demonstrate significant differences in between systems. isothermal diagram MO−NaO−2H2O system is dominated large lamellar liquid crystalline that shows an ideal swelling up to high water contents. Stable vesicles dominant...
An amyloid form of the protein α-synuclein is major component intraneuronal inclusions called Lewy bodies, which are neuropathological hallmark Parkinson's disease (PD). α-Synuclein known to associate with anionic lipid membranes, and interactions between aggregating cellular membranes thought be important for PD pathology. We have studied molecular determinants adsorption monomeric planar model composed zwitterionic phosphatidylcholine alone or in a mixture phosphatidylserine (relevant...
We have studied nucleation and crystal growth of calcium carbonate on hard surfaces, i.e. stainless steel silica, at different temperatures, in relation to the corresponding bulk processes, using scanning electron microscopy (SEM), X-ray diffraction (XRD), ellipsometry. In solution, a mixture all three crystalline polymorphs, calcite, aragonite, vaterite, as well amorphous particles was observed 25 °C, while 55 °C aragonite calcite crystals dominated. On surfaces only were whereas adsorbed...
Inhaled antibiotic treatment of cystic fibrosis-related bacterial biofilm infections is challenging because the pathological environment lungs. Here, we present an "environment-adaptive" nanoparticle composed a solid poly lactic-co-glycolic acid (PLGA) core and mucus-inert, enzymatically cleavable shell d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for site-specific delivery antibiotics to biofilms via aerosol administration. The hybrid nanoparticles with ultrasmall size were...
We discuss different nonequilibrium mechanisms by which bulk aggregates directly modify, and can even control, the interfacial structure morphology of an oppositely charged polyelectrolyte/surfactant (P/S) mixture. Samples are categorized at air/water interface with respect to dynamic changes in phase behavior, composition, sample history using complementary surface-sensitive techniques. First, we show that spontaneously interact adsorption layer retained it this process occurs most readily...
Lipid nanoparticles of reversed internal phase structures, such as cubic micellar (I2) structure show good drug loading ability peptides and proteins well some small molecules. Due to their controllable size inner morphology, are suitable for delivery using several different administration routes, including intravenous, intramuscular, subcutaneous injection. A very interesting system in this regard, is the two component soy phosphatidylcholine (SPC)/glycerol dioleate (GDO) system, which...
The lipid liquid crystalline sponge phase (L3) has the advantages that it is a nanoscopically bicontinuous bilayer network able to accommodate large amounts of water and easy manipulate due its fluidity. This paper reports on detailed characterization L3 phases with channels enough encapsulate bioactive macromolecules such as proteins. aqueous behavior novel mixture system, consisting diglycerol monooleate (DGMO), mono-, di- triglycerides (Capmul GMO-50) was studied. In addition, sponge-like...
The last couple of decades have seen an explosion novel colloidal drug delivery systems, which been demonstrated to increase efficacy, reduce side-effects, and provide various other advantages for both small-molecule biomacromolecular drugs. interactions systems with biomembranes are increasingly recognized play a key role efficient eradication pathogens cancer cells, as well intracellular protein nucleic acid In parallel, there has broadening methodologies investigating such systems. For...