A5089656590- Surfactants and Colloidal Systems
- Spectroscopy and Quantum Chemical Studies
- Lipid Membrane Structure and Behavior
- Analytical Chemistry and Chromatography
- Molecular spectroscopy and chirality
- Enzyme Catalysis and Immobilization
- NMR spectroscopy and applications
- Mesoporous Materials and Catalysis
- Nanoparticle-Based Drug Delivery
- Electrochemical sensors and biosensors
- Liquid Crystal Research Advancements
- Electron Spin Resonance Studies
- Thermodynamic properties of mixtures
- Advanced NMR Techniques and Applications
- Chemical and Physical Properties in Aqueous Solutions
- Protein Interaction Studies and Fluorescence Analysis
- Advancements in Transdermal Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Drug Delivery Systems
- Material Dynamics and Properties
- Food Chemistry and Fat Analysis
- Electrostatics and Colloid Interactions
- Electrochemical Analysis and Applications
- Polymer Surface Interaction Studies
- Microbial Metabolic Engineering and Bioproduction
University of Cagliari
2013-2023
Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase
2004-2023
Istituto Nazionale di Fisica Nucleare, Sezione di Cagliari
2001-2017
International Centre for Genetic Engineering and Biotechnology
2010
Uppsala University
2008
Australian National University
1995-2008
University of Florence
1997-2008
University of Molise
1999-2006
Solvay (Italy)
2004
Physical Sciences (United States)
2004
The points of zero charge/potential proteins depend not only on pH but also how they are measured. They background salt solution type and concentration. protein isoelectric point (IEP) is determined by electrokinetical measurements, whereas the isoionic (IIP) potentiometric titrations. Here we use titration zeta potential (ζ) measurements at different NaCl concentrations to study systematically effect ionic strength IEP IIP bovine serum albumin (BSA) aqueous solutions. It found that high...
Protein solubility studies below the isoelectric point exhibit a direct Hofmeister series at high salt concentrations and an inverse low concentrations. The efficiencies of different anions measured by needed to effect precipitation fixed cations are usual (Cl(-) > NO(3)(-) Br(-) ClO(4)(-) I(-) SCN(-)). sequence is reversed This has been known for over century. Reversal not peculiar proteins. Its origin poses key test any theoretical model. Such specific ion effects in cloud points lysozyme...
In this work, monoolein-based cubosomes were doped with two fluorescent probes, namely, fluorescein and dansyl, properly modified a hydrocarbon chain to increase their encapsulation efficiency within the monoolein palisade. The same nanocarriers also loaded quercetin, hydrophobic molecule potential anticancer activity. Particularly, probe successfully exploited for single living cell imaging. physicochemical photophysical characterizations reported here, along well-known ability of in...
This work was devoted to the development of a new type lipid-based (cubosome) theranostic nanoparticle able simultaneously host camptothecin, potent anticancer drug, and squarain-based NIR-emitting fluorescent probe. Furthermore, confer targeting abilities on these nanoparticles, they were dispersed using mixtures Pluronic F108 folate-conjugated in appropriate ratios. The physicochemical characterization, performed via SAXS, DLS, cryo-TEM techniques, proved that aqueous dispersions such...
Biofuels could be defined as the fuels of future, although much work is still required before they will replace fossil fuels. In this review, lignocellulosic biomass based on straw and related crops wastes described concerning its lignin contents, structure, properties, an overview means current predictable delignification protocols presented. The discussion focused herbaceous monocot materials their available pretreatments (physical, chemical, enzymatic), with special emphasis fungal...
In this work a sample of SBA-15 mesoporous silica was synthesized and characterized by TEM, XRD, N2 adsorption. The had high value specific surface area (1007 m2 g-1) total pore volume (2.1 cm3 g-1). diameter 67 Å, so it large enough to accommodate protein molecules inside the channels. Immobilization physical adsorption commercial lipase preparation from Mucor javanicus performed at different pH values (pH 5−8). 6 gave highest loading hydrolytic activity corresponding biocatalyst. Chemical...
The effects of weak and strong electrolytes on the enzymatic activity Candida rugosa lipase are explored. Weak electrolytes, used as buffers, set pH, while regulate ionic strength. interplay between pH strength has been assumed to be determinant activity. In experiments that probe activities by varying these parameters, there little attention focused role specific electrolyte effects. Here we show both buffers choice background ion strongly affect lipase. here shown dramatic at high salt...
The effect of electrolytes on pH measurements via glass electrodes is explored with solutions buffered at 7 (phosphate and cacodylate). Salt buffer concentrations are varied. Direct reverse Hofmeister effects observed. phenomena significant for salt above 0.1 M below 20 mM. Changes in measured show up most strongly anions. They can be related to the usual physicochemical parameters (anion molar volumes, refractivity, surface tensions) that characteristic series. correlate anionic excess...
Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) lauroylcholine chloride (LCh), are investigated for structural features short-term in vitro cytotoxicity. Depending on the morphologies lipid NPs (cubosomes liposomes) obtained, as demonstrated cryo-TEM images. Although offer many advantages medical applications various chemicals used their preparation under...
Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic light scattering (ELS) are used to study specific-ion binding. The effect is appreciable at a physiological concentration of 0.1 M. We found that anions bind the protein surface an acidic pH, where carries positive charge (Z(p) > 0), according Hofmeister series (Cl(-) < Br(-) NO(3)(-) I(-) SCN(-)), as well isoionic point = 0). results obtained require critical interpretation. measurements...
Specific ion effects on the Brownian motion of BSA protein under physiological conditions give new useful insights into electrolyte–protein interactions and molecular mechanisms involved in Hofmeister effect.
Here, we describe a novel monoolein-based cubosome formulation engineered for possible theranostic applications in oncology. The Docetaxel-loaded nanoparticles were stabilized water by mixture of commercial Pluronic (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer) F108 (PF108) and rhodamine- folate-conjugated PF108 so that the possess targeting, therapeutic, imaging properties. Nanoparticles investigated DLS, cryo-TEM, SAXS to confirm their structural...
Hofmeister series reversal can occur with change in pH, or increase salt concentration. The phenomena are a challenge for any theory of ion specific effects. Recent theoretical work predicts how complex interplay between ionic sizes, hydration and dispersion forces explains reversal. Electrophoretic mobility measurements on lysozyme suspensions reported here consistent the theory.
The solubilization of vitamin K1 (VK1), a highly hydrophobic molecule, into 1-monoolein/water (MO/W) system, is investigated by NMR self-diffusion, small angle X-ray scattering (SAXS), optical microscopy, and electrochemical methods. various MO/W phases, namely L2, Lα, CG, CD, can accommodate different amounts VK1. In particular, the Lα cubic CG phases solubilize up to 8 5 wt % VK1, respectively, without modifing microstructure substantially. By contrast, CD phase only about 1 Larger...
Divalent metal–acetate solutions have been investigated by the x-ray scattering technique and 13C NMR spectroscopy. The aim of this work was to study complex formation between divalent metals a typical organic complexing ligand in concentrated aqueous solutions. correlation functions analysis structure agree with literature information that species present solution are Me(H2O)2+6 , Me(H2O)6−z(CH3COO)2+−zz Me(H2O)6−2z(CH3COO)2+−zz . Good agreement experimental data is achieved through model...