A5007296483- Salivary Gland Disorders and Functions
- Nanoparticle-Based Drug Delivery
- Mesoporous Materials and Catalysis
- Glycosylation and Glycoproteins Research
- Renal and related cancers
- Polymer Surface Interaction Studies
- Cellular Mechanics and Interactions
- Salivary Gland Tumors Diagnosis and Treatment
- Pediatric Urology and Nephrology Studies
- Proteoglycans and glycosaminoglycans research
- Heat shock proteins research
- Urological Disorders and Treatments
- Caveolin-1 and cellular processes
- Antimicrobial Peptides and Activities
- Biochemical effects in animals
- S100 Proteins and Annexins
- Advanced Drug Delivery Systems
- Gold and Silver Nanoparticles Synthesis and Applications
- Nuclear Structure and Function
- Surfactants and Colloidal Systems
- RNA Research and Splicing
- Lipid metabolism and biosynthesis
- Advanced Electron Microscopy Techniques and Applications
- Trace Elements in Health
- Analytical chemistry methods development
University of Cagliari
2014-2024
Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase
2019-2022
Istituto Nazionale di Fisica Nucleare, Sezione di Cagliari
2010-2021
Ordered mesoporous materials (OMMs) are interesting matrixes for nanomedicine applications such as innovative drug delivery systems. Here, we compare the behavior of widely studied SBA-15 silica with that less investigated MSE (a periodic organosilica whose silicon atoms alternatively connected by means −Si−O−Si− and −Si−CH2−CH2−Si− groups) toward adsorption (pH 7.0 9.6) in vitro release 7.4; T = 37 °C) an antimicrobial protein (hen egg white lysozyme). Both OMMs have a hexagonal ordered...
Mesoporous silica nanoparticles (MSNs), based on the MCM-41 matrix, were functionalized with amino groups, and then hyaluronic acid (HA) or chitosan (CHIT) to fabricate bioactive conjugates. The role of functional groups toward cytotoxicity cellular uptake was investigated using 3T3 mouse fibroblast cells. A very high biocompatibility MSN-NH2, MSN-HA MSN-CHIT matrices assessed through MTS biological assay Coulter counter evaluation. No significant differences in data arise from presence...
Thymosin β4 (Tβ4) was extracted forty years agofrom calf thymus. Since then, it has been identified as a G-actin binding protein involved in blood clotting, tissue regeneration, angiogenesis, and anti-inflammatory processes. Tβ4 also implicated tumor metastasis neurodegeneration. However, the precise roles mechanism(s) of action these processes remain largely unknown, with being insufficient to explain multi-actions. Here we identify for first time important role mechanism ferroptosis, an...
TEM images of human lysozyme loaded on SBA-15 mesoporous silica were obtained through the immunogold staining (IGS) method. IGS is based specific interaction between proteins and colloidal gold-conjugated antibodies. Clear evidence that protein molecules are adsorbed both external inner pore surface presented.
Compartmentalized systems produced via the layer-by-layer (LbL) self-assembly method have been by alternatively depositing alginate and chitosan layers onto cores of liposomes. The combination dynamic light scattering (DLS), ζ potential, transmission electron microscopy (TEM) techniques provides detailed information on stability, dimensions, charge, wall thickness these polyelectrolyte globules. TEM microphotographs demonstrate presence nanocapsules with an average diameter below 300 nm a...
Aurivillius oxides ferroelectric layered materials are formed by bismuth oxide and pseu-do-perovskite layers. They have a good ionic conductivity, which is beneficial for various photo-catalyzed reactions. Here, we synthesized ultra-thin nanosheets of two different oxides, Bi
Histatins are a family of salivary proteins with bactericidal and fungicidal activities that contribute to the innate defense oral cavity. present in serous granules parotid submandibular glands. The important role histatins saliva, limited information on their cellular subcellular distribution, prompted us further define localization major Immunogold-silver staining 1- micro m sections plastic-embedded tissue anti-histatin antibody revealed histatin immunoreactivity acinar cells glands,...
J Oral Pathol Med (2010) 39: 715–721 Background: Salivary dysfunction and oral disorders have been described in both type 1 2 diabetes mellitus. However, the cellular molecular consequences of on tissues remain to be ascertained. The purpose this investigation was study, by means electron microscopy, morphologic changes that occur salivary glands during diabetes. Methods: Biopsy samples parotid were excised from non-diabetic diabetic (type 2) consenting patients processed standard methods...
The effect of buffer species on biomolecules and biomolecule-nanoparticle interactions is a phenomenon that has been either neglected, or not understood. Here, we study the formation BSA protein corona (PC) around amino-functionalized mesoporous silica nanoparticles (MSN-NH
The human salivary mucins MG1 and MG2 are well characterized biochemically functionally. However, there is disagreement regarding their cellular glandular sources. aim of this study was to define the localization distribution these two in glands using a postembedding immunogold labeling method. Normal obtained at surgery were fixed 3% paraformaldehyde-0.1% glutaraldehyde embedded Lowicryl K4M or LR Gold resin. Thin sections labeled with rabbit antibodies an N-terminal synthetic peptide MG2,...
Due to its actin-sequestering properties, thymosin beta-4 (Tβ4) is considered play a significant role in the cellular metabolism. Several physiological properties of Tβ4 have been reported;, however, many questions concerning function remain be ascertained. To better understand this small peptide we analyzed by means transmission immunoelectron microscopy techniques ultrastructural localization HepG2 cells. Samples cells were fixed mixture 3% formaldehyde and 0.1% glutaraldehyde 0.1 M...
Mesoporous silica nanoparticles (MSN) were synthesised and functionalised with triethylenetetramine (MSN-TETA). The samples fully characterised (transmission electron microscopy, small angle X-ray scattering, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential nitrogen adsorption/desorption isotherms) used as carriers for the adsorption of antimicrobial drug sulphamethizole (SMZ). SMZ loading, quantified by UV–Vis was higher on MSN-TETA (345.8 mg g−1) compared...
Nephrogenesis is mainly characterized by the interaction of two distinct renal constituents, ureteric bud and metanephric mesenchyme. In this paper we describe means light electron microscopic techniques morphological events that take place during early stages cap mesenchymal formation. Samples normal tissue were excised from newborn NOD mice processed standard microscopy techniques. all samples examined detected presence several aggregates in different differentiation. They varied small...