A5053953521- Salivary Gland Disorders and Functions
- Hemoglobin structure and function
- Advanced Proteomics Techniques and Applications
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- S100 Proteins and Annexins
- Erythrocyte Function and Pathophysiology
- Cellular Mechanics and Interactions
- Microfluidic and Capillary Electrophoresis Applications
- Glycosylation and Glycoproteins Research
- Analytical Chemistry and Chromatography
- Neonatal Health and Biochemistry
- Hemoglobinopathies and Related Disorders
- Neonatal Respiratory Health Research
- Mass Spectrometry Techniques and Applications
- Protein purification and stability
- Protein Hydrolysis and Bioactive Peptides
- Cancer Cells and Metastasis
- Metabolism and Genetic Disorders
- Biochemical effects in animals
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Peptidase Inhibition and Analysis
- Protein Structure and Dynamics
Fondazione Santa Lucia
2019-2025
Istituti di Ricovero e Cura a Carattere Scientifico
1986-2024
European Brain Research Institute
2022-2023
Institute of Chemistry of Molecular Recognition
2013-2022
National Research Council
2000-2022
National Research Council
2021
Università Cattolica del Sacro Cuore
2010-2020
Agostino Gemelli University Polyclinic
1998-2018
University of Brescia
2018
Friedrich-Alexander-Universität Erlangen-Nürnberg
2018
To elucidate the localization of post-translational modifications different classes human salivary proteins and peptides (acidic basic proline-rich (PRPs), Histatins, Statherin, P-B peptide, "S type" Cystatins) a comparative reversed phase HPLC-ESI-MS analysis on intact enriched granule preparations from parotid submandibular glands as well parotid, submandibular/sublingual (Sm/Sl), whole saliva was performed. The main results this study indicate following. (i) Phosphorylation all peptides,...
Thiourea tasting can be predictive of individual differences in bitter taste responses, general food preferences and eating behavior, could correlated with saliva chemical composition. We investigated the possible relationship between PROP responsiveness salivary proteome subjects genotyped for TAS2R38 gustin gene polymorphisms. Taste perception intensity evoked by NaCl solutions was measured sixty-three volunteers (21 males, 42 females, age 25±3 y) to establish their taster status, 24...
Physiological variability of the naturally occurring, human salivary secretory peptidome was studied as a function age. The qualitative and quantitative changes occurring in secretion proteins/peptides specific to oral cavity (i.e., basic proline-rich proteins, acidic phosphoproteins, statherin, peptide P-B, cystatins, histatins) were investigated by high-performance liquid chromatography-electrospray ionization-mass spectrometry 67 subjects aged between 3 44 years. Subjects divided into...
Increasing evidences support the notion that impairment of intracellular degradative machinery is responsible for accumulation oxidized/misfolded proteins ultimately results in deposition protein aggregates. These events are key pathological aspects "protein misfolding diseases", including Alzheimer disease (AD). Interestingly, Down syndrome (DS) neuropathology shares many features with AD, such as both amyloid plaques and neurofibrillary tangles. Studies from our group others demonstrated,...
Cluster of differentiation 44 (CD44) is a non-kinase cell surface glycoprotein. It overexpressed in several types, including cancer stem cells (CSCs). Cells overexpressing CD44 exhibit CSC traits, such as self-renewal, epithelial-mesenchymal transition (EMT) capability, and resistance to chemo- radiotherapy. The role maintaining stemness the function tumor progression accomplished by binding its main ligand, hyaluronan (HA). HA-CD44 complex activates signaling pathways that lead...
The metabolic behaviour of human erythrocytes has been investigated with particular regard to the effect their oxygenation state. Experiments performed at high phosphate concentration (80 mM) within pH range 7.0-7.8 on (HOS) and low (LOS) oxygen saturation showed that any value: (1) glucose consumption was independent state; (2) pentose pathway (PPP) flux about 2 times higher in HOS than LOS At (1.0 PPP doubled as well erythrocytes, whereas decrease more marked Metabolism approached under...
The systematic search by tandem mass spectrometry of human saliva from four different subjects, 136 possible fragments originated histatin 3, allowed the detection 24 peptides. They include, with exception 4, all known 3 fragments, namely histatins 5–12 and peptides corresponding to 15–24, 26–32, 29–32 residues, 13 new 1–11, 1–12, 1–13, 5–13, 6–11, 6–13, 7–11, 7–12, 7–13, 14–24, 14–25, 15–25, 28–32 residues 3. On contrary, none 119 1, including 2, was detected. results suggest that genesis...
Thirteen samples of human normal whole saliva were analyzed by RP-HPLC−ESI−MS and MALDI-TOF−MS to investigate the basic proline-rich protein complex. Between known basic-PRPs P-B, P-C (or IB-8b), P-D IB-5), P-E IB-9), P-F IB-8c), P-H IB-4), IB-6, II-2, IB-1, IB-8a glucosylated identified, whereas II-1, IB-7, PA, D1-A peptides not detected. Some detected masses attributable putatively ascribed II-2 IB-1 nonphosphorylated, missing C-terminal arginine residue, 1-62 fragment named P-J peptide. A...
The acidic‐soluble protein content of human gingival crevicular fluid was analyzed by reverse‐phase high‐performance liquid chromatography (RP‐HPLC), and the eluent deriving from separation directly introduced into an ion‐trap mass spectrometer through electrospray ionization (ESI‐IT MS). By this technique molecular weight peptides/proteins determined with a precision ≈ 1/10,000 amu. On basis chromatographic behavior knowledge value, some peptides proteins soluble in acidic solution were...
Abstract Objective To investigate the effect of pilocarpine on salivary peptide and protein profile in patients with primary Sjögren's syndrome (SS) to study differences between SS, SS associated other rheumatic diseases, healthy control subjects. Methods Saliva specimens were obtained from 9 patients, secondary 10 controls. Samples analyzed for levels 62 different proteins using high‐performance liquid chromatography coupled mass spectrometry a spectrometer equipped an electrospray...
RP-HPLC-ESI-MS profile of naturally occurring salivary peptides subjects with autistic spectrum disorder [ASD; N = 27: 12 diagnosis autism, 1 Asperger, 14 pervasive developmental disorders not otherwise specified (PDD-NOS)] was compared to that age-matched controls the goal identifying differences could turn out become hallmarks at least a subgroup ASD individuals. Phosphorylation level four specific phospho-peptides, namely statherin, histatin (both, p < 0.0001) and acidic proline-rich...
The acidic soluble fraction of whole saliva type 1 diabetic children was analyzed by reversed phase (RP)(1)-HPLC-ESI-MS and compared with that sex- age-matched control subjects. Salivary proline-rich phosphoproteins (aPRP), histatins, α-defensins, salivary cystatins, statherin, peptide P-B (P-B), beta-thymosins, S100A8 S100A9*(S100A9* corresponds to S100A9 vairant lacking the first four amino acids), as well some naturally occurring peptides derived from phosphoproteins, peptide, were...
Human cervical mucus is a heterogeneous mixture of mucin glycoproteins whose relative concentration changes during the ovulatory phases, thereby producing different aggregation structures that can periodically permit transit spermatozoa for fertilization. In preovulatory phase, arranged in compact fiber-like where sperm hindered. Previously, through observations made fixed and dehydrated samples, permissive structure phase was attributed to larger diameters pores network. Instead, by means...
Saliva is a body fluid of unique composition devoted to protect the mouth cavity and digestive tract. Our high performance liquid chromatography (HPLC)-electrospray ionization-MS analysis acidic soluble fraction saliva from preterm human newborn surprisingly revealed more than 40 protein masses often undetected in adult saliva. We were able identify following proteins: stefin A B, S100A7 (two isoforms), S100A8, S100A9 (four S100A11, S100A12, small proline-rich 3 lysozyme C, thymosins β(4)...