A5042692218- Enzyme Structure and Function
- Trypanosoma species research and implications
- Genomics and Phylogenetic Studies
- Genetic and phenotypic traits in livestock
- Cancer Research and Treatments
- Research on Leishmaniasis Studies
- DNA and Nucleic Acid Chemistry
- Asymmetric Synthesis and Catalysis
- Immune Cell Function and Interaction
- Amino Acid Enzymes and Metabolism
- Enzyme Production and Characterization
- Advanced biosensing and bioanalysis techniques
- Fluorine in Organic Chemistry
- Microbial Metabolism and Applications
- Insect symbiosis and bacterial influences
- Synthetic Organic Chemistry Methods
- Genetic Mapping and Diversity in Plants and Animals
- RNA and protein synthesis mechanisms
- Vector-Borne Animal Diseases
- Chemical Synthesis and Reactions
- DNA Repair Mechanisms
- Biochemical and Molecular Research
- Oxidative Organic Chemistry Reactions
- Livestock Farming and Management
- Microbial Metabolic Engineering and Bioproduction
Roslin Institute
2015-2024
University of Edinburgh
2015-2024
University of Exeter
2015
Simon Fraser University
1995-1999
Purdue University West Lafayette
1992-1994
University of Madras
1979-1989
Voluntary Health Services Hospital
1979
Universität Greifswald
1973
Abstract Despite only 8% of cattle being found in Europe, European breeds dominate current genetic resources. This adversely impacts research other important global breeds, especially those from Africa for which genomic resources are particularly limited, despite their disproportionate importance to the continent’s economies. To mitigate this issue, we have generated assemblies African been integrated with data 294 diverse into a graph genome that incorporates diversity. We illustrate how...
Transcriptional reprogramming is integral to effective plant defense. Pathogen effectors act transcriptionally and posttranscriptionally suppress defense responses. A major challenge understanding disease responses discriminating between transcriptional associated with microbial-associated molecular pattern (MAMP)-triggered immunity (MTI) that orchestrated by effectors. high-resolution time course of genome-wide expression changes following Pseudomonas syringae pv tomato DC3000 the...
Cattle are an economically important domestic animal species. In vitro 2D cultures of intestinal epithelial cells or cell lines have been widely used to study function and host–pathogen interactions in the bovine intestine. However, these lack cellular diversity encountered epithelium, physiological relevance monocultures transformed is uncertain. Little also known factors that influence differentiation homeostasis few cell-specific markers can distinguish different lineages reported. Here...
Abstract More people globally depend on the water buffalo than any other domesticated species, and as most closely related species to cattle they can provide important insights into shared evolutionary basis of domestication. Here, we sequence genomes 79 across seven breeds compare patterns between breed selective sweeps with those seen for 294 representing 13 global breeds. The genomic regions under selection significantly overlap linked stature in human genetic studies, a disproportionate...
Summary Pathogens target phytohormone signalling pathways to promote disease. Plants deploy salicylic acid ( SA )‐mediated defences against biotrophs. antagonize immunity by activating jasmonate signalling, for example Pseudomonas syringae pv. tomato DC 3000 produces coronatine COR ), a jasmonic JA ) mimic. This study found unexpected dynamics between , and co‐operation JAZ repressor proteins during infection. We used systems‐based approach involving targeted hormone profiling,...
Antigenic variation is employed by many pathogens to evade the host immune response, and Trypanosoma brucei has evolved a complex system achieve this phenotype, involving sequential use of variant surface glycoprotein (VSG) genes encoded from large repertoire ~2,000 genes. T. express multiple, sometimes closely related, VSGs in population at any one time, ability resolve analyse diversity been limited. We applied long read sequencing (PacBio) VSG amplicons generated blood extracted batches...
Water buffalo is a cornerstone livestock species in many low- and middle-income countries, yet major gaps persist its genomic characterization, complicated by the divergent karyotypes of two sub-species (swamp river). Such complexity makes water particularly good candidate for use graph genomics, which can capture variation missed linear reference approaches. However, utility this approach to improve has been largely unexplored. We present comprehensive pangenome that integrates four newly...
Trypanosoma congolense is a principal agent causing livestock trypanosomiasis in Africa, costing developing economies billions of dollars and undermining food security. Only the diamidine diminazene phenanthridine isometamidium are regularly used, resistance widespread but poorly understood. We induced stable T. strain IL3000 vitro. There was no cross-resistance with drugs, melaminophenyl arsenicals, oxaborole trypanocides, or except close analogs DB829 DB75. Fluorescence microscopy showed...
For the first time we have defined naïve, central memory, effector memory and differentiated porcine CD8 T cells analyzed their distribution in lymphoid respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three nucleoprotein (NP) epitopes. The hierarchy response to epitopes changes during different tissues. Most NP-specific broncho-alveolar lavage (BAL) lung are tissue resident (TRM) that express CD69 downregulate CD45RA CCR7. isolated from BAL genes...
Human and animal African trypanosomiasis (HAT & AAT, respectively) remain a significant health economic issue across much of sub-Saharan Africa. Effective control AAT potential eradication HAT requires affordable, sensitive specific diagnostic tests that can be used in the field. Small RNAs blood or serum are attractive disease biomarkers due to their stability, accessibility available technologies for detection. Using RNAseq, we have identified trypanosome small RNA present at high levels...
Mucosal-associated invariant T (MAIT) cells are a population of innate-like that utilize semi-invariant cell receptor (TCR) α chain and restricted by the highly conserved antigen presenting molecule MR1. MR1 presents microbial riboflavin biosynthesis derived metabolites produced bacteria fungi. Consistent with their ability to sense ligands from bacterial sources, MAIT have been associated immune response variety infections, such as Mycobacterium spp., Salmonella spp. Escherichia coli. To...
The African buffalo (Syncerus caffer) is a wild bovid with historical distribution across much of sub-Saharan Africa. Genomic analysis can provide insights into the evolutionary history species, and key selective pressures shaping populations, including assessment population level differentiation, fragmentation, genetic structure. In this study we generated highest quality de novo genome assembly (2.65 Gb, scaffold N50 69.17 Mb) to date, sequenced further 195 genomes from species...
Animal African Trypanosomiasis (AAT) is a debilitating livestock disease prevalent across sub-Saharan Africa, main cause of which the protozoan parasite Trypanosoma congolense . In comparison to well-studied T brucei , there major paucity knowledge regarding biology Here, we use combination omics technologies and novel genetic tools characterise core metabolism in mammalian-infective bloodstream-form parasites, test whether metabolic differences compared impact upon sensitivity inhibition....
Abstract Es wurden 164 Humanmilchproben von 96 Müttern auf Rückstände an chlorierten Kohlenwasserstoffen dünnschichtchromatographisch untersucht. Die Nachweisgrenze betrug 0,005 ppm o, p′‐DDT, 0,01 p, p′‐DDT bzw. p′‐DDD, 0,01–0,05 p′‐DDE, γ‐HCH β‐HCH sowie 0,1 für PCB. Bei allen wurde bei 95 65 PCB, 64 β‐HCH, 12 spurenweise p′‐DDD und 3 nachgewiesen. Durchschnittlich enthielt die Humanmilch 0,09 0,21 p′‐DDE (0,32 Gesamt‐DDT), 0,07 Der Gesamt‐DDT‐Gehalt in erhöhte sich mit zunehmendem Alter...
Abstract The African buffalo ( Syncerus caffer ) is a wild bovid with historical distribution across much of sub-Saharan Africa. Genomic analysis can provide insights into the evolutionary history species, and key selective pressures shaping populations, including assessment population level differentiation, fragmentation, genetic structure. In this study we generated highest quality de novo genome assembly (2.65 Gb, scaffold N50 69.17 Mb) to date, sequenced further 195 genomes from species...
East Coast fever (ECF) in cattle is caused by the Apicomplexan protozoan parasite Theileria parva, transmitted three-host tick Rhipicephalus appendiculatus. The African buffalo (Syncerus caffer) natural host for T. parva but does not suffer disease, whereas ECF often fatal cattle. genetic relationship between populations circulating and poorly understood, has been studied sympatric This study aimed to determine diversity of buffalo, an area where livestock co-exist with adjacent Serengeti...
En traitant la cetone de Wieland Miescher par le dimethyl-2,2 propanediol on obtient un monoacetal que l'on reduit Li/NH 3
Abstract Introducing the concept of “dinucleotide” as helical repeat, theoretical attempts have been made to determine possible single and double stranded structures by using parameter calculations model building investigations. By virtue its flexible framework, dinucleotide repeat offers a much greater scope finding new secondary structural forms for nucleic acids. Considering only those conformations which show tendency at least partial base overlap does it has predict poly(dinucleotide)...