A5087355711- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Pregnancy and Medication Impact
- Pregnancy and preeclampsia studies
- Systemic Sclerosis and Related Diseases
- Endometriosis Research and Treatment
- COVID-19 Impact on Reproduction
- Cytokine Signaling Pathways and Interactions
- Preterm Birth and Chorioamnionitis
- Immune Response and Inflammation
- melanin and skin pigmentation
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Reproductive Physiology in Livestock
- Cell Adhesion Molecules Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Diabetes and associated disorders
- Regulation of Appetite and Obesity
- Galectins and Cancer Biology
- Pancreatic function and diabetes
- Infant Nutrition and Health
- Biochemical Analysis and Sensing Techniques
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
Umeå University
2014-2024
University Hospital of Umeå
2007-2016
Extracellular vesicles (EVs) are membraneous released by a variety of cells into their microenvironment. Recent studies have elucidated the role EVs in intercellular communication, pathogenesis, drug, vaccine and gene-vector delivery, as possible reservoirs biomarkers. These findings generated immense interest, along with an exponential increase molecular data pertaining to EVs. Here, we describe Vesiclepedia, manually curated compendium (lipid, RNA, protein) identified different classes...
Herein, we describe a novel approach in the search for prostate cancer biomarkers, which relies on transcriptome within tumour exosomes. As proof-of-concept, show presence of two known PCA-3 and TMPRSS2:ERG exosomes isolated from urine patients, showing potential diagnosis monitoring patients status.
Immune evasion from NK surveillance related to inadequate NK-cell function has been suggested as an explanation of the high incidence relapse and fatal outcome many blood malignancies. In this report we have used Jurkat Raji cell lines a model for studies NKG2D receptor-ligand system in T-and B leukemia/lymphoma. Using real-time quantitative RT-PCR immunoflow cytometry show that cells constitutively express mRNA protein stress-inducible ligands MICA/B ULBP1 2, up-regulate expression...
During mammalian pregnancy maternal-fetal tolerance involves a number of immunosuppressive factors produced by placenta. Recently, placenta-derived exosomes have emerged as new immune regulators in the maternal tolerance. Exosomes are membrane nanovesicles with defined morphology, which secreted from endosomal multivesicular bodies (MVB) upon fusion plasma membrane. Previously, we reported that MHC class I chain-related (MIC) proteins A and B, human ligands activating NK cell receptor NKG2D,...
Adeno-associated virus (AAV) vectors have shown remarkable efficiency for gene delivery to cultured cells and in animal models of human disease. However, limitations AAV vectored transfer exist after intravenous transfer, including off-target (e.g., liver) low transduction target tissue. Here, we show that during production, a fraction are associated with microvesicles/exosomes, termed vexosomes (vector-exosomes). capsids the surface interior microvesicles were visualized using electron...
Tumor-derived exosomes, which are nanometer-sized extracellular vesicles of endosomal origin, have emerged as promoters tumor immune evasion but their role in prostate cancer (PC) progression is poorly understood. In this study, we investigated the ability tumor-derived exosomes to downregulate NKG2D expression on natural killer (NK) and CD8+ T cells. an activating cytotoxicity receptor whose aberrant loss plays important suppression. Using flow cytometry, found that produced by human PC...
Abstract Apoptosis is crucially important in mediating immune privilege of the fetus during pregnancy. We investigated expression and vitro apoptotic activity two physiologically relevant death messengers, TNF family members Fas ligand (FasL) TRAIL human early term placentas. Both molecules were intracellularly expressed, confined to late endosomal compartment syncytiotrophoblast, tightly associated generation secretion placental exosomes. Using immunoelectron microscopy, we show that FasL...
The syncytiotrophoblast (STB) of human placenta constitutively produces and secretes extracellular vesicles different size, morphology function that enter the maternal circulation, participate in maternal-fetal cross-talk during pregnancy. Syncytiotrophoblast-derived microvesicles/microparticles (STBM) are larger microvesicles (0.2-2 μm) shed by apical plasma membrane STB as a result cell activation turnover. Simultaneously with STBM shedding, exosomes--nanosized (30-100/150 nm)...
Abstract Mammalian pregnancy is an intriguing immunological phenomenon where the semiallogeneic fetus not rejected. Tolerance toward involves a number of mechanisms associated with modifications immune status mother. In this study, we strongly suggest novel mechanism for fetal evasion maternal attack, based on engagement and down-regulation activating NK cell receptor NKG2D PBMC by soluble MHC class I chain-related proteins A B (collectively termed MIC). similar escape pathway was previously...
The local immune privilege of the fetus is created by placenta. Fas ligand (FasL) expression in trophoblast has been implied as one mechanisms fetal tolerance. However, membranal FasL failed to explain this role FasL. Two objections can be raised: (1) there have contradictions considering which cells, syncytiotrophoblast (ST) or cytotrophoblast, express FasL; (2) vivo and vitro studies shown that form evokes inflammatory response thus may promote rejection. Using different assays...
Galectin-1 (gal-1) is expressed at the feto-maternal interface and plays a role in regulating maternal immune response against placental alloantigens, contributing to pregnancy maintenance. Both decidua placenta contribute gal-1 expression may be important for regulation. The of within considered relevant cell-adhesion invasion trophoblasts, but evasion machinery exhibited by trophoblast cells remains elucidated. In this study, we analyzed preimplantation human embryos first-trimester...
Abstract Exosomes are specialized, nanometer‐sized extracellular vesicles of endosomal origin actively secreted into the space by a variety cells under normal and pathological conditions. have recently emerged as important intercellular communicators modulators diverse mechanisms cellular responses. Characterization their composition function will open possibilities for new diagnostic methods promising therapeutic approaches based on nanobiology. This unit provides standard isolation...
PROBLEM: To examine the cytokine gene expression in γδT‐cells from human early pregnancy decidua. METHOD OF STUDY: The messenger RNA (mRNA) isolated decidual T‐cell receptor (TCR)γδ + /CD56 and TCRγδ single positive cells was investigated with a panel of primers probes selected to distinguish between T helper (Th)1, Th2, Th3 regulatory T‐cells (Tr1) type immune response using real‐time quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR). RESULTS: express almost exclusively...
The mononuclear lymphoid cell population in human pregnant uterus mucosa, decidua, from early normal pregnancies was studied phenotypically and functionally. phenotype determined situ by immunohistochemistry, isolated decidual preparations immunofluorescence flow cytometry. A mild isolation procedure of gentle mechanical disruption followed density gradient centrifugation used. Leukocytes comprised a large part the tissue. They were present aggregates mainly situated adjacent to glandular...
Cancers constitutively produce and secrete into the blood other biofluids 30–150 nm-sized endosomal vehicles called exosomes. Cancer-derived exosomes exhibit powerful influence on a variety of biological mechanisms to benefit tumors that them. We studied immunosuppressive ability epithelial ovarian cancer (EOC) two cytotoxic pathways importance for anticancer immunity—the NKG2D receptor-ligand pathway DNAM-1-PVR/nectin-2 pathway. Using exosomes, isolated from EOC tumor explant cell-line...
Citation Dimova T, Nagaeva O, Stenqvist A‐Christin, Hedlund M, Kjellberg L, Strand Dehlin E, Mincheva‐Nilsson L. Maternal Foxp3 Expressing CD4 + CD25 and − Regulatory T‐Cell Populations are Enriched in Human Early Normal Pregnancy Decidua: A Phenotypic Study of Paired Decidual Peripheral Blood Samples. Am J Reprod Immunol 2011; 66 (Suppl. 1): 44–56 Problem T cells (Treg cells), a small subset maintaining tolerance by immunosuppression, proposed contributors to the survival fetal...
Abstract Human decidual lymphocytes from early, normal pregnancy were characterized in situ with respect to ultrastructure and distribution of subsets. The isolated gamma delta T cells was also studied. CD45+ comprised 11 +/- 2% all cells. majority localized large lymphoid cell clusters (LCC), near endometrial glands, or as intraepithelial (IEL) glandular epithelium. major populations LCC CD56+TCR-gamma delta+ cells, CD56+ TCR-alpha beta+CD4+ beta+CD8+ All expressed activation markers...
Exosomes are nanosized membrane-bound vesicles that released by various cell types and capable of carrying proteins, lipids RNAs which can be delivered to recipient cells. play a role in intercellular communication have been described mediate immunologic information. In this article we report the first isolation characterization exosomes from human thymic tissue. Using electron microscopy, particle size determination, density gradient measurement, flow cytometry, proteomic analysis microRNA...
Endometriosis, affecting 10% of women, is defined as implantation, survival, and growth endometrium-like/endometriotic tissue outside the uterine cavity, causing inflammation, infertility, pain, susceptibility to ovarian cancer. Despite extensive studies, its etiology pathogenesis are poorly understood largely unknown. The prevailing view that immune system endometriosis patients fails clear ectopically disseminated endometrium from retrograde menstruation. Exosomes small extracellular...
Objective To determine the relationship between vascular function and inflammatory response in systemic sclerosis (SSc), to investigate whether production of endothelial-derived nitric oxide (NO) is disturbed this disease. Methods We measured plasma nitrate, urinary excretion both nitrate cGMP, soluble adhesion molecules endothelial origin patients with SSc age- sex-matched controls compared these levels groups. Additionally, we performed correlation analysis how variables were related one...
Abstract The uterine mucosa in pregnancy, the decidua, allows placenta formation and survival of fetus despite fact that it is semiallogeneic. Decidua contains large numbers lymphocytes, which CD56+ cells dominate, followed by T expressing either alpha beta or gamma delta TCR. We have investigated developmental relationship between CD56- TCR delta-expressing early pregnancy decidua using dual labeling immunoelectron microscopy, immunoflow cytometry, cell fractionation. Lymphocyte...
The functional properties, regarding parasite growth inhibition in vitro , the cytotoxic potential and cytokine profiles of human γδ + αβ T cells, T‐cell lines clones stimulated with Plasmodium falciparum‐ antigen‐or mitogen were investigated. Using reverse transcriptase‐polymerase chain reaction (RT‐PCR) specific primers, mRNA for cytolytic molecules perforin, granzyme A B, Fas ligand (FasL) detected both γδ‐ αβT cells. Despite this fact, only γδT cells inhibited, Vδ1 Vδ2 asexual blood...