A5019783706- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Plant Virus Research Studies
- Biosensors and Analytical Detection
- Plant and Fungal Interactions Research
- Bacterial Identification and Susceptibility Testing
- Tuberculosis Research and Epidemiology
- Nail Diseases and Treatments
- Helminth infection and control
- SARS-CoV-2 detection and testing
- Mycorrhizal Fungi and Plant Interactions
- Lichen and fungal ecology
- Insect-Plant Interactions and Control
- Mycotoxins in Agriculture and Food
- HIV/AIDS drug development and treatment
- Parasite Biology and Host Interactions
- Antibiotic Use and Resistance
- Mycobacterium research and diagnosis
- Insect and Pesticide Research
- Yeasts and Rust Fungi Studies
- Pneumonia and Respiratory Infections
- COVID-19 diagnosis using AI
Institut National de Santé Publique du Québec
2015-2025
Clinical and Laboratory Standards Institute
2023-2024
Ste. Anne's Hospital
2015-2023
CS Diagnostics
2023
Université de Montréal
2017
University of Georgia
2017
Association des Médecins Vétérinaires du Québec
2017
McGill University
2004-2015
Institut National de la Recherche Scientifique
2005
Abstract Background The liver fluke Fasciola hepatica is a major pathogen of livestock worldwide, causing huge economic losses to agriculture, as well 2.4 million human infections annually. Results Here we provide draft genome for F. , which find be among the largest known genomes at 1.3 Gb. This size cannot explained by duplication or expansion single repeat element, and remains paradox given burden it may impose on egg production necessary transmit infection. Despite potential inbreeding...
Extracellular vesicles (EVs) released by parasites have important roles in establishing and maintaining infection. Analysis of the soluble vesicular secretions adult Fasciola hepatica has established a definitive characterization total secretome this zoonotic parasite. secretes at least two subpopulations EVs that differ according to size, cargo molecules site release from The larger are specialized cells line parasite gastrodermus contain zymogen 37 kDa cathepsin L peptidase performs...
Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid, highly accurate, and cost-effective method for routine identification wide range microorganisms. We carried out side by comparative evaluation the performance Bruker Biotyper versus VITEK MS large diverse collection Most difficult and/or unusual microorganisms, well commonly encountered microorganisms were selected, including Gram-positive negative bacteria, mycobacteria,...
The CLSI epidemiological cutoff values (ECVs) of antifungal agents are available for various Candida spp., Aspergillus and the Mucorales. However, those categorical endpoints have not been established Fusarium mostly due to difficulties associated with collecting sufficient MICs clinical isolates identified according currently recommended molecular DNA-PCR-based identification methodologies. MIC distributions were 53 dimerum species complex (SC), 10 F. fujikuroi, 82 proliferatum, 20...
Clinical breakpoints (CBPs) have not been established for the Mucorales and any antifungal agent. In lieu of CBPs, epidemiologic cutoff values (ECVs) are proposed amphotericin B, posaconazole, itraconazole four species. Wild-type (WT) MIC distributions (organisms in a species-drug combination with no detectable acquired resistance mechanisms) were defined available pooled CLSI MICs from 14 laboratories (Argentina, Australia, Canada, Europe, India, Mexico, United States) as follows: 10...
The rapid pace of name changes medically important fungi is creating challenges for clinical laboratories and clinicians involved in patient care. We describe two sources change which have different drivers, at the species versus genus level. Some suggestions are made here to reduce number changes. urge taxonomists provide diagnostic markers taxonomic novelties. Given instability phylogenetic trees due variable taxon sampling, we advocate maintain genera largest possible size. Reporting...
Candida auris is an emerging multidrug-resistant yeast that has been systematically incorrectly identified by phenotypic methods in clinical microbiology laboratories. The Vitek 2 automated identification system (bioMérieux) recently included C. its database (version 8.01). We evaluated the performance of YST ID card to identify and related species. A panel 44 isolates species (C. auris, n = 35; haemulonii, 5; duobushaemulonii, 4) were tested three different hospital-based Among 35 yielded...
Amphotericin B remains an important treatment for multidrug resistant Candida auris. Antifungal susceptibility testing of amphotericin in C. auris can vary depending on the methodology used. Here, we compared Etest method and Clinical Laboratory Standards Institute broth microdilution reference against 60 clinical isolates from four major clades. The minimum inhibitory concentrations differed significantly by (p-value, <0.0001) discrepancies were observed interpretation resistance...
ABSTRACT Chromoblastomycosis, a fungal neglected tropical disease, is acquired through traumatic inoculation and clinically characterized by chronic granulomatous infection of the skin subcutaneous tissue. Fonsecaea pedrosoi most commonly reported etiologic agent globally. Itraconazole considered first-line therapy, but successful treatment with terbinafine, voriconazole, posaconazole has been reported. F. minimum inhibitory concentration (MIC) data are limited, epidemiological cutoffs...
The poly(A)-binding protein (PABP) is an important translation initiation factor that binds to the polyadenylated 3' end of mRNA. We have previously shown PABP2 interacts with RNA-dependent RNA polymerase (RdRp) and VPg-Pro turnip mosaic virus (TuMV) within virus-induced vesicles. At least eight PABP isoforms are produced in Arabidopsis thaliana, three which (PABP2, PABP4 PABP8) highly broadly expressed probably constitute bulk required for cellular functions. Upon TuMV infection, increase...
The recent emergence of the pathogenic fungus Cryptococcus gattii in Pacific Northwest (PNW) resulted numerous investigations into epidemiological and enzootic impacts, as well multiple genomic explorations three primary molecular subtypes that were discovered. These studies lead to general conclusion identified likely emerged out Brazil. Here, we conducted dating analyses determine ages various lineages seen PNW propose hypothetical causes for dispersal events. Bayesian evolutionary...
Abstract Antimicrobial resistance (AMR) is a global threat. To optimize the use of our antifungal arsenal, we need rapid detection and monitoring tools that rely on high-quality AMR mutation data. Here, performed thorough manual curation published mutations in fungal pathogens to produce FungAMR reference dataset. A total 462 papers were curated, leading 54,666 entries all classified with degree evidence supports their role resistance. covers 92 species, 202 genes 184 drugs. We combined...
Azole resistance among Aspergillus fumigatus isolates is a growing concern worldwide. Induction of mutations during azole therapy, environment-acquired caused by fungicides and intrinsic cryptic Fumigati species all contribute to the burden resistance. However, there lack data in Canada on this emerging threat.To gain insights into magnitude mechanisms resistance, 14 year collection section comprising 999 from 807 patients at Montreal hospital was screened for were investigated with combined...
To identify the prevalence of C. auris in Canadian patients who are potentially at risk for colonization, we screened 488 were either hospitalized abroad, had a carbapenemase-producing organism (CPO), or units with high antifungal use. Two colonized auris; both received healthcare India and CPO. Among 35 recently Indian subcontinent CPO infected, was 5.7%.
Candida auris is an emerging yeast that associated with antifungal resistance and healthcare-associated outbreaks. From 2012 to 2019, there were 24 known cases of C. colonization or infection in Canada. Isolates from axilla/groin (n = 6), ear 5), blood 4), toe 2), a variety other sites 7). Canadian isolates belonged the four main genomic clades: Clade I (formerly called South Asian clade, n 12), II (East Asian, 3), III (African, IV (South American, 5). within each clade clonal; however,...
Background Acute invasive fungal infections of the head and neck secondary to tyrosine kinase inhibitors are rare potentially life-threatening events. Case presentation We report a case mucormycosis thyroid gland in patient known for chronic lymphocytic leukemia receiving ibrutinib who presented with rapidly growing nodule dysphonia. An acute infection was identified on core needle biopsy; confirmed culture. The successfully treated surgical debridement long-term antifungal therapy....
Cryptococcus gattii infection in mammals and birds has been confined historically to tropical subtropical regions Australia, Southeast Asia, Africa, South America. Since the early 2000s, numerous reports describe emergence of C. on Pacific Coast North We report a an 8-year-old male citron-crested cockatoo (Cacatua sulphurea citrinocristata) hatched Canadian raised province Québec, Canada. The bird developed slow growing ulcerated, fleshy, crusty, hemorrhagic mass infiltrating left lower...
Bronchoalveolar lavage is usually employed for molecular diagnosis of
Invasive fungal infections by opportunistic yeasts have increased concomitantly with the growth of an immunocompromised patient population. Misidentification can lead to inappropriate antifungal treatment and complications. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy is a promising method for rapid accurate identification microorganisms. ATR-FTIR standalone, inexpensive, reagent-free technique that provides results within minutes after initial culture. In...