A5071828712- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Infectious Diseases and Mycology
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Essential Oils and Antimicrobial Activity
- Mycotoxins in Agriculture and Food
- Lichen and fungal ecology
- Phenothiazines and Benzothiazines Synthesis and Activities
- Mycorrhizal Fungi and Plant Interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Chemical synthesis and alkaloids
- Cancer therapeutics and mechanisms
- Synthesis and biological activity
- Actinomycetales infections and treatment
- Yeasts and Rust Fungi Studies
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Neutropenia and Cancer Infections
- Protist diversity and phylogeny
- Synthesis and Biological Evaluation
- Fisheries and Aquaculture Studies
- Enterobacteriaceae and Cronobacter Research
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2024
Hospital Ramos Mejía
2014-2024
Centro Científico Tecnológico - San Juan
2017-2022
Westerdijk Fungal Biodiversity Institute
2002-2017
Centro Científico Tecnológico - Santa Fe
2014
Centro Científico Tecnológico - Tucumán
2009
GGz centraal
2002-2007
Radboud University Nijmegen
2002-2007
Administración Nacional de Laboratorios e Institutos de Salud
2005-2007
Radboud University Medical Center
2002-2007
The in vitro susceptibilities of 66 molecularly identified strains the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on nuclear ribosomal large subunit reveal taxon-specific susceptibility profiles. impressive phylogenetic diversity reflected differing at family, genus, species levels. Amphotericin B most active drug, though somewhat...
The rapid pace of name changes medically important fungi is creating challenges for clinical laboratories and clinicians involved in patient care. We describe two sources change which have different drivers, at the species versus genus level. Some suggestions are made here to reduce number changes. urge taxonomists provide diagnostic markers taxonomic novelties. Given instability phylogenetic trees due variable taxon sampling, we advocate maintain genera largest possible size. Reporting...
Recent changes in the Fungal Code of Nomenclature and developments molecular phylogeny are about to lead dramatic naming medically important molds yeasts. In this article, we present a widely supported simple proposal prevent unnecessary nomenclatural instability.
ABSTRACT The present paper includes a meta-analysis of literature data on 318 species fungi belonging to 34 orders in their response 8 antifungal agents (amphotericin B, caspofungin, fluconazole, itraconazole, ketoconazole, posaconazole, terbinafine, and voriconazole). Main trends MIC results at the ordinal level were visualized. European Committee Antimicrobial Susceptibility Testing Clinical & Laboratory Standards Institute (CLSI) clinical breakpoints used as staff gauge evaluate...
Clinical echinocandin resistance among Candida glabrata strains is increasing, especially in the United States. Antifungal susceptibility testing considered mandatory to guide therapeutic decisions. However, these methodologies are not routinely performed hospital setting due their complexity and time needed obtain reliable results. Echinocandin failure C. linked exclusively Fks1p Fks2p amino acid substitutions, detection of such substitutions would serve as a surrogate marker identify...
Abstract Fungi in the order Chaetothyriales are renowned for their ability to cause human infections. Nevertheless, they not regarded as primary pathogens, but rather opportunists with a natural habitat environment. Extremotolerance is major trend order, quite different from black yeasts Capnodiales which focus on endurance, an important additional parameter advancing toxin management. In ancestral ecology of rock colonization, association metabolite-producing lichens significant....
ABSTRACT To develop new approaches for the treatment of invasive infections caused by Aspergillus fumigatus , in vitro interactions between itraconazole (ITZ) and seven different nonantimicrobial membrane-active compounds—amiodarone (AMD), amiloride, lidocaine, lansoprazole (LAN), nifedipine (NIF), verapamil, fluphenazine—against ITZ-susceptible ITZ-resistant (ITZ-R) strains were evaluated checkerboard microdilution method based on National Committee Clinical Laboratory Standards M-38A...
The increase in fungal infections and the change epidemiology is caused by extensive use of antifungal agents to treat that are being diagnosed severly immunocompromised hosts. In addition, opportunistic resistant drugs have become increasingly common, armamentarium for treatment remains limited. A possible approach overcoming these problems combine drugs, especially if mechanisms action different. vitro test first step evaluate combinations. this chapter, three most frequently used...
The antifungal activity of six drugs was evaluated against 41 clinical and environmental isolates Cladophialophora carrionii its sister species C. yegresii. Drugs tested, including their ranges, were: 16–0.016 µg/ml for amphotericin B (AMB), itraconazole (ITZ) voriconazole (VCZ), 8–0.008 terbinafine (TBF), 64–0.063 flucytosin (5-FC) fluconazole (FCZ). Strains were tested according to the CLSI guidelines (M38A). MIC Gmeans strains in were; 0.02 TBF ITZ, 0.07 VCZ, 0.49 5FC, 6.14 FCZ 9.42 AMB....
Black yeasts morphologically identified as Exophiala cf. spinifera were re-analyzed on the basis of morphology, nutritional physiology and ITS rDNA sequencing data. Ribosomal small subunit (SSU) DNA sequence data confirmed that strains belonged to a clade containing ex-type strain E. spinifera. Variation in Internal Transcribed Spacer (ITS) region was studied within this group. Five infraspecific groups delineated. Phylogenetic analysis SSU revealed several analyzed located at considerable...
ABSTRACT Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungi Cladophialophora bantiana , Rhinocladiella mackenziei and Exophiala dermatitidis. Despite combination of surgery with antifungal treatment, prognosis continues be poor, mortality rates ranging from 50 70%. Therefore, search for more-appropriate therapeutic approach urgently needed. Our in vitro studies showed that amphotericin B flucytosine against these...
The results of 5034 blood cultures, implementing a lysis-centrifugation method with saponin, are summarized in this paper. Three hundred and twenty-two samples (6.3%) obtained from pool human immunodeficiency virus (HIV)-positive patients yielded fungi. Cryptococcus neoformans was isolated 199 (3.95%), Histoplasma capsulatum 95 (1.89%). Candida parapsilosis 12 (0.23%), C. albicans 7 (0.13%), tropicalis 2, krusei 1, guillermondii Prototheca wickerhamii 4 (0.07%). Blood cultures were positive...
The phenothiazine compounds chlorpromazine and trifluopherazine are antipsychotic agents that exhibit antimicrobial activity against bacteria, some protozoa yeasts. Data of filamentous fungi lacking. in vitro postantifungal effect (PAFE) was determined Aspergillus species, zygomycetes Scedosporium species. In susceptibility testing performed with CLSI M38A the PAFE previously established methods. Both drugs inhibited growth all tested at concentrations 16 to 64 microg ml(-1). For species...
Cryptococcosis causes disseminated disease in AIDS patients. In contrast to what occurs laboratory conditions, a large capsule is produced by Cryptococcus neoformans vivo during infection. The aim of this study was compare the vitro activity different antifungal agents against 34 clinical isolates C. var. grubii without or with induction (CLSI, CLSI-C, respectively), following CLSI M27A3 document. Capsule obtained addition NaHCO(3) and incubation CO(2). geometric means MICs, µg ml(-1), for...
ABSTRACT An in vitro method for determination of postantifungal effect (PAFE) molds was developed by using three isolates each Aspergillus fumigatus , A. flavus terreus nidulans and ustus . MICs amphotericin B itraconazole were determined National Committee Clinical Laboratory Standards guidelines (M38-P). The inoculum prepared RPMI 1640 broth buffered with MOPS (morpholinepropanesulfonic acid) at pH 7.0, conidia exposed to concentrations 4, 1, 0.25 times the MIC, 2, 1 h 37°C. same procedure...
Antifungal susceptibility profiles were determined for 16 strains of the black yeast Exophiala spinifera applying different temperature regimens. Fluconazole was least effective in vitro. Lowest minimal inhibitory concentration (MIC) values found with itraconazole. The activities antifungal agents against environmental and clinical similar. Post-antifungal effect (PAFE) four drugs 11 strains. PAFE observed only amphotericin B, extended inhibition times seen at high drug concentrations.
The combined effects of antifungal and antibiotic drugs against Exophiala spinifera were evaluated in vitro by the chequer-board method, calculated as a fractional inhibitory concentration (FIC) index. Amphotericin B was with flucytosine ciprofloxacin, whereas itraconazole levofloxacin, lomefloxacin sulfadiazine. Synergic observed for combinations ciprofloxacin amphotericin flucytosine. No antagonism any combination tested.