A5019037976- Extracellular vesicles in disease
- Mosquito-borne diseases and control
- Complement system in diseases
- Malaria Research and Control
- interferon and immune responses
- Immune cells in cancer
- Parasites and Host Interactions
- Cytokine Signaling Pathways and Interactions
- SARS-CoV-2 and COVID-19 Research
- Parasite Biology and Host Interactions
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Venomous Animal Envenomation and Studies
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- Antibiotic Use and Resistance
- Nosocomial Infections in ICU
- Viral Infections and Vectors
- RNA Research and Splicing
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2022-2024
Barcelona Institute for Global Health
2020-2024
Universitat de Barcelona
2020-2024
Abstract Plasmodium vivax is the most widely distributed human malaria parasite. Previous studies have shown that circulating microparticles during P. acute attacks are indirectly associated with severity. Extracellular vesicles (EVs) therefore major components of plasma holding insights into pathological processes. Here, we demonstrate plasma-derived EVs from patients ( Pv EVs) preferentially uptaken by spleen fibroblasts h SFs) as compared to uptake healthy individuals. Moreover, this...
Abstract Parasites are responsible for the most neglected tropical diseases, affecting over a billion people worldwide (WHO, 2015) and accounting billions of cases year several millions deaths. Research on extracellular vesicles (EVs) has increased in recent years demonstrated that EVs shed by pathogenic parasites interact with host cells playing an important role parasite's survival, such as facilitation infection, immunomodulation, parasite adaptation to environment transfer drug...
Plasmodium vivax is the most widely distributed human malaria parasite with 7 million annual clinical cases and 2.5 billion people living under risk of infection. There an urgent need to discover new antigens for vaccination as only two vaccine candidates are currently in trials. Extracellular vesicles (EVs) small membrane-bound involved intercellular communication initially described reticulocytes, host cell P. , a selective disposal mechanism transferrin receptor (CD71) maturation...
Latent liver stages termed hypnozoites cause relapsing Plasmodium vivax malaria infection and represent a major obstacle in the goal of elimination. Hypnozoites are clinically undetectable, presently, there no biomarkers this persistent parasite reservoir human liver. Here, we have identified proteins associated with extracellular vesicles (EVs) secreted from vivo infections exclusively containing hypnozoites. We used P. vivax-infected liver-chimeric (huHEP) FRG KO mice treated...
The spleen is a secondary lymphoid organ with multiple functions including the removal of senescent red blood cells and coordination immune responses against blood-borne pathogens, such as malaria parasites. Despite major role spleen, study its function in humans limited by ethical implications to access human tissues. Here, we employed multiparameter flow cytometry combined cell purification techniques determine populations from transplantation donors. Spleen immuno-phenotyping showed that...
Human malaria caused by Plasmodium vivax infection (vivax malaria) is a major global health issue. It the most geographically widespread form of disease, accounting for 7 million annual clinical cases, majority cases in America and Asia an estimation over 2.5 billion people living under risk infection. The general perception towards has shifted recently, following series reports, from being viewed as benign to recognition its potential more severe manifestations including fatal cases....
Recent studies indicate that human spleen contains over 95% of the total parasite biomass during chronic asymptomatic infections caused by Plasmodium vivax . Previous have demonstrated extracellular vesicles (EVs) secreted from infected reticulocytes facilitate binding to fibroblasts (hSFs) and identified genes whose expression was dependent on an intact spleen. Here, we characterize P. spleen-dependent hypothetical gene (PVX_114580). Using CRISPR/Cas9, PVX_114580 integrated into falciparum...
Melanoma differentiation-associated protein 5 (MDA5) induces type I interferons (IFNs) after the recognition of viral RNA. In addition, gain-of-function mutations in interferon induced with helicase C domain 1 (IFIH1) gene, which encodes MDA5, lead to interferonopathies. Here, we show that Mda5 is highly expressed murine macrophages and regulated by pro-inflammatory stimuli such as cytokines IFN-α IFN-γ, TLR ligand LPS, a mimic dsRNA, poly(I:C). induction mediated through production reactive...
Extracellular vesicles (EVs) released by virus-infected cells have the potential to encapsulate viral peptides, a characteristic that could facilitate vaccine development. Furthermore, plasma-derived EVs may elucidate pathological changes occurring in distal tissues during infections. We hypothesized molecular characterization of isolated from COVID-19 patients would reveal peptides suitable for Blood samples were collected three cohorts: severe (G1), mild/asymptomatic cases (G2), and...