A5000847386- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Nanoparticle-Based Drug Delivery
- Spondyloarthritis Studies and Treatments
- CAR-T cell therapy research
- Agricultural economics and policies
- Hematopoietic Stem Cell Transplantation
- Bone and Joint Diseases
- Extracellular vesicles in disease
- Polish socio-economic development
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Neonatal Respiratory Health Research
- Radiation Therapy and Dosimetry
- Autoimmune and Inflammatory Disorders Research
- Neonatal and Maternal Infections
- Synthesis and Biological Evaluation
- Nanoparticles: synthesis and applications
- Preterm Birth and Chorioamnionitis
- Tissue Engineering and Regenerative Medicine
- Laser-Ablation Synthesis of Nanoparticles
- HIV Research and Treatment
- Click Chemistry and Applications
Jagiellonian University
2015-2024
Krakow Cardiovascular Research Institute
2020
Collegium Medicum in Bydgoszcz
2020
University of Michigan–Ann Arbor
2000
University of Wisconsin–Madison
2000
CA-170 is currently the only small-molecule modulator in clinical trials targeting PD-L1 and VISTA proteins – important negative checkpoint regulators of immune activation. The reported therapeutic results to some extent mimic those FDA-approved monoclonal antibodies overcoming limitations high production costs adverse effects latter. However, no conclusive biophysical evidence proving binding hPD-L1 has ever been presented. Using well-known vitro methods: NMR assay, HTRF cell-based...
We describe a new class of potent PD-L1/PD-1 inhibitors based on terphenyl scaffold that is derived from the rigidified biphenyl-inspired structure. Using in silico docking, we designed and then experimentally demonstrated effectiveness terphenyl-based scaffolds inhibiting PD-1/PD-L1 complex formation using various biophysical biochemical techniques. also present high-resolution structure PD-L1 with one our most to identify key PD-L1/inhibitor interactions at molecular level. In addition,...
Immune checkpoint blockade is one of the most promising strategies cancer immunotherapy. However, unlike classical targeted therapies, it currently solely based on expensive monoclonal antibodies, which often inflict immune-related adverse events. Herein, we propose a novel small-molecule inhibitor at clinically relevant immune checkpoint, PD-1/PD-L1. The compound capable disrupting PD-1/PD-L1 complex by antagonizing PD-L1 and, therefore, restores activation T cells similarly to while being...
Novel functionalized nanoparticles, with toxicity controlled by laser irradiation, are perspective agents for potential (MRI)-guided stimulated chemo-photothermal treatment of cancer.
Programmed death-1 (PD-1) receptor system represents a part of recently reported immunoregulatory pathway. PD-1 is an immune checkpoint molecule, which plays important role in downregulating the proinflammatory activity. Until recently, expression was not established on cells preterm infants. The study objectives were to confirm receptors monocytes isolated from very low birth weight newborns (VLBW), and analyze their during first week life late-onset sepsis. Peripheral blood mononuclear 76...
Abstract The novel iron-oxide nanoparticles (NPs) stabilized with triethanolammonium oleate were produced. specimens divided into two groups: the top NPs (extracted from supernatant) and bottom (nanoparticles sedimented at of flask during centrifugation), respectively. XRD Mössbauer studies revealed a presence Fe 3 O 4 phase in both types nanoparticles. Furthermore, formation maghemite layer on surface was observed. Average particle sizes determined TEM lower than superparamagnetic limit for...
Abstract To determine whether monocytes can be generated from CD34+ hematopoietic progenitors in large numbers, cord blood cells were first expanded for 3–10 days X-VIVO 10 medium supplemented with FCS, stem cell factor (SCF), thrombopoietin (TPO), and Flt-3 Ligand (Flt-3L), then differentiated IMDM SCF, Flt-3L, IL-3 M-CSF 7–14 days. These two step cultures resulted up to a 600-fold mean increase of total CD14+ cells. Using this approach, subpopulations obtained: CD14+CD16− CD14++CD16+...
Tumour cells release membrane micro(nano)fragments called tumour-derived microvesicles (TMV) that are believed to play an important role in cancer progression. TMV suppress/modify antitumour response of the host, but there is also some evidence for their direct interaction with cells. In patients present body fluid and tumour microenvironment. The study aimed at characterization whole types/subpopulations, not only exosomes, from newly established gastric cell line (called GC1415) define...
Abstract Background New nanophotosensitizers for photothermal cancer therapy (PTT) are still sought. In this paper we propose fancy shaped, non agglomerated core/shell PtAu NRs nanoraspberries (PtAu NRs) as potential in PTT. Results Light microscopy images of two colon cell lines (SW480, SW620) showed, that the laser irradiation combined with caused visible changes morphology. Fourier Transform InfraRed (FTIR) and Raman spectroscopies showed chemical DNA, phospholipids, lipids protein...
<b>Aims:</b> To investigate the relationship between bone-derived osteocalcin (OC), osteoprotegerin (OPG), Receptor Activator of Nuclear Factor NF-ĸB ligand (RANKL), and fat tissue-derived leptin adiponectin with a clinical outcome type 1 diabetes mellitus (T1DM) in children adolescents. <b>Methods:</b> 78 patients (43 girls 35 boys), aged 11.5±4.3 years T1DM 11 age- BMI-matched controls were included into study. Patients divided 3 groups according to HbA1c level, I – below 7% [53 mmol/mol],...
We present new small-molecular probes targeting the human PD-L1 protein. The molecules were designed by incorporating a newly discovered N-methylmorpholine substituent into known biphenyl-based structure. Four prototype derivatives of 4-methyl-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-carbonitrile (STD4), comprising morpholine fused with biphenyl core at different orientations first verified for their potential binding to using molecular docking method. A more favorable 7-phenyl derivative STD4...
High enantioselectivity has been demonstrated in the protonation of N,N-diisopropyl amides (Table 1, entries 1−4, 7, and 10−13) derived from certain β,γ unsaturated acids. Depending on double bond geometry degree substitution at γ-carbon, γ-protonation can be a competing reaction case aliphatic substrates 12, 14b, 14d, 18. The evidence is most consistent with mechanism that involves proton transfer 1a to mixed aggregate consisting enolate 4a lithiated amide 5, but direct not ruled out.
Abstract An easy synthesis method of hollow, porous gold nanoparticles (AuHP NPs) with controlled diameter and pores sizes a wide range light absorbance (continuous between 500 900 nm) is presented together the explanation nanoparticle formation mechanism. The NPs were investigated using transmission electron microscopy (TEM) combined selected area diffraction patterns, X-ray ultraviolet–visible spectroscopy. TEM images showed that changing temperature allows to obtain AuHP from 35 ± 4 nm at...
Enhancing the effectiveness of colorectal cancer treatment is highly desirable. Radiation-based anticancer therapy—such as proton therapy (PT)—can be used to shrink tumors before subsequent surgical intervention; therefore, improving this crucial. The addition noble metal nanoparticles (NPs), acting radiosensitizers, increases PT therapeutic effect. Thus, in paper, effect novel, gold–platinum nanocauliflowers (AuPt NCs) on efficiency determined. For purpose, crystalline, 66-nm fancy shaped,...
One of the most common anticancer therapies is photothermal therapy (PTT). The effectiveness PTT depends on photosensitizer being a molecule which toxic for cancer cells after electromagnetic wave irradiation. Therefore, simulation was performed in this work two colon (SW480 and SW620) using platinum nanoparticles (Pt NPs). Interestingly, literature dependence between synthesis method properties Pt NPs not discussed. Consequently, paper, we evaluated synthesized by different methods: polyol...
Noble metal nanoparticles, such as gold (Au NPs), platinum (Pt or palladium (Pd due to their highly developed surface, stability, and radiosensitizing properties, can be applied support proton therapy (PT) of cancer. In this paper, we investigated the potential bimetallic, c.a. 30 nm PtAu PdAu nanocomplexes, synthesized by green chemistry method not used previously radiosensitizers, enhance effect colorectal cancer PT in vitro. The obtained nanomaterials were characterized scanning...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) selectively eliminates tumor cells. However, the short biological half-life of this molecule limits its potential use in clinic. Our aim was to construct a recombinant strain nonpathogenic Lactococcus lactis bacteria as vector for effective and prolonged human TRAIL production. Herein, we examined expression secretion conditions leading production biologically active protein vitro. The soluble TRAIL-cDNA (hsTRAIL-cDNA) with...
Nano-sized radiosensitizers can be used to increase the effectiveness of radiation-based anticancer therapies. In this study, bimetallic, ~30 nm palladium-platinum nanoparticles (PdPt NPs) with different nanostructures (random nano-alloy NPs and ordered core-shell were prepared. Scanning transmission electron microscopy (STEM), selected area diffraction (SAED), energy-dispersive X-ray spectroscopy (EDS), zeta potential measurements, nanoparticle tracking analysis (NTA) provide...