A5039249276- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Neuroendocrine regulation and behavior
- Parallel Computing and Optimization Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Nicotinic Acetylcholine Receptors Study
- Interconnection Networks and Systems
- Distributed and Parallel Computing Systems
- Nerve injury and regeneration
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- interferon and immune responses
- Ultrasound and Cavitation Phenomena
- Hepatitis C virus research
- Embedded Systems Design Techniques
- Sleep and Wakefulness Research
- Pain Management and Placebo Effect
- Anesthesia and Pain Management
- Distributed systems and fault tolerance
- Hepatitis B Virus Studies
- RNA regulation and disease
National University of Singapore
2015-2024
Fortis Hospital
2024
Imperial College Healthcare NHS Trust
2024
Cincinnati Children's Hospital Medical Center
2024
Fortis Healthcare
2024
Royal Brompton & Harefield NHS Foundation Trust
2018-2020
JSS Academy of Higher Education and Research
2020
Indian Spinal Injuries Centre
2020
Pain Clinic of India
2020
Ashwini Hospital
2020
N-methyl-D-aspartate (NMDA) receptors have been implicated in many neurological disorders. Although NMDA are best known for their high calcium permeability, the recently discovered NR3 subunits, NR3A and NR3B, shown to reduce permeability of receptor. Thus, subunits may be important players modulating synaptic plasticity neurons. NR3B expression rodent human brain has studied, little is about its distribution different cell types. Here we used immunolabeling with a specific antibody together...
Reactive astrogliosis is a critical process in neuropathological conditions and neurotrauma. Although it has been suggested that confers neuroprotective effects, the exact genomic mechanism not explored. The prevailing dogma of role inhibition axonal regeneration challenged by recent findings rodent model's spinal cord injury, demonstrating its neuroprotection properties. We examined whether their potentials can be identify human reactive astrocytes vitro. Here, was induced with IL1β. Within...
Limbic structures including the hippocampus are thought to be involved in pain though not much is known of their neuronal responses noxious stimuli. In this report we show that a prolonged and substantial depression dorsal hippocampal CA1 pyramidal cell population spike produced by brief but intense stimulus applied tail lightly anaesthetized rats. This temperature-dependent habituates subsequent stimuli more than 1 h later. Further, absent when heat presence theta rhythm.
Chronic lymphocytic leukemia (CLL), a malignant B-cell disorder, is characterized by heterogeneous clinical course. Two-dimensional nano liquid chromatography (2D-nano–LC) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) (LC–MALDI) was used to perform qualitative and quantitative analysis on cellular extracts from 12 primary CLL samples. We identified 728 proteins quantified 655 using isobaric tag-labeled extracts. Four...
Elevated iron deposition has been reported in Parkinson's disease (PD). However, the route of uptake leading to high substantia nigra is unresolved. Here, we show a mechanism enhanced Fe 2+ via S-nitrosylation divalent metal transporter 1 (DMT1). While DMT1 could be S-nitrosylated by exogenous nitric oxide donors, human PD brains, endogenously was detected postmortem nigra. Patch-clamp electrophysiological recordings and assays confirmed increased Mn or through DMT1. We identified two major...
c-fos expression mapping and electrophysiological recording experiments were done to clarify the role of A1 noradrenergic cell group in vasopressin response hypotensive hemorrhage. In pentobarbital-anesthetized rats, moderate severe hemorrhages simulated by brief occlusion inferior vena cava sufficient reduce mean arterial pressure approximately 50 or 30 mmHg, respectively. Both stimuli significantly increased number region catecholamine cells displaying Fos-like immunoreactivity, this...
Abstract The patterns of hippocampal neuronal loss and rewiring the dentate gyrus (DG) were studied in mouse model temporal lobe epilepsy at 2 months after pilocarpine‐induced status epilepticus (PISE). NeuN immunocytochemistry showed two damage, i.e., type 1 with partial pyramidal neurons CA3 area almost compete neurons. Anterograde tracing Phaseolus vulgaris leucoagglutinin (PHA‐L) demonstrated that, different rostrocaudal segments hippocampus, associational commissural connections DG...
Abstract The present study explored the functional details of influence medial septal region (MSDB) on spectrum nociceptive behaviours by manipulating intraseptal GABAergic mechanisms. Results showed that formalin-induced acute nociception was not affected microinjection bicuculline, a GABA A receptor antagonist, or selective lesion neurons. Indeed, responses were dissociated from regulation sensorimotor behaviour and generation theta-rhythm mechanisms in MSDB. attenuated unconditioned...
To ascertain: 1) the frequency of thrombocytopenia and heparin-induced thrombocytopenia; 2) positive predictive value Pretest Probability Score in identifying 3) clinical outcome adult patients receiving venovenous- or venoarterial-extracorporeal membrane oxygenation, compared with cardiopulmonary bypass.A single-center, retrospective, observational cohort study from January 2016 to April 2018.Tertiary referral center for cardiac respiratory failure.Patients who received extracorporeal...
Abstract The induction of hippocampal theta by reticular stimulation involves a relay to the hippocampus via posterior hypothalamic–supramammillary region and then medial septum. Interestingly, sensory‐ or behaviour‐induced is accompanied suppression field CA1 synaptic responses. In present study, performed on anaesthetized rats, we observed that also induced pyramidal cell population spike corresponding dendritic excitatory postsynaptic potential evoked CA3 stimulation. This was at...
Abstract Significant reduction in glutamate receptor 1 (GluR1)‐ and GluR2/3‐immunopositive neurons was demonstrated the hilus of dentate gyrus mice killed on days 1, 7 60 after pilocarpine‐induced status epilepticus (PISE). In addition, GluR1 GluR2/3 immunostaining strata oriens, radiatum lacunosum moleculare areas CA1–3 decreased drastically PISE. Neuronal loss observed above regions may account, at least part, for a decrease GluR immunoreactivity. By contrast, many GluR1‐immunopositive...
In the present study using extracellular electrophysiological recording techniques, we explored temporal characteristics of hippocampal theta activation in relation to formalin nociception. Results indicate that, compared hind paw injection saline, behaving rat evoked biphasic increase duration dorsal CA1 theta. Such an broadly paralleled animal behavioral activation, especially lick and moment-to-moment agitated behaviors. Correspondingly, theta-modulated cell firing was observed following...
The medial septum is anatomically and functionally linked to the hippocampus, a region implicated in nociception. However, role of nociception remains unclear. To investigate rats, muscimol, GABA agonist, or zolpidem, positive allosteric modulator GABA(A) receptors, was microinjected into attenuate activity neurons region. Electrophysiological studies anesthetized rats indicated that muscimol evoked stronger longer-lasting suppression septal-mediated activation hippocampal theta field than...
Neuropathic pain remains underrecognised and ineffectively treated in chronic sufferers. Consequently, their quality of life is considerably reduced, substantial healthcare costs are incurred. The anatomical location must be identified for definitive diagnosis, but current neuropsychological tools cannot do so. Matrix metalloproteinases (MMP) thought to maintain peripheral neuroinflammation, MMP-12 elevated particularly such pathological conditions. Magnetic resonance imaging (MRI) the...