A5078534826- Prostate Cancer Treatment and Research
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Prostate Cancer Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Digestive system and related health
- Radiomics and Machine Learning in Medical Imaging
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Neuroendocrine Tumor Research Advances
- Phagocytosis and Immune Regulation
- Tissue Engineering and Regenerative Medicine
- CNS Lymphoma Diagnosis and Treatment
- Cardiac, Anesthesia and Surgical Outcomes
- Cancer, Hypoxia, and Metabolism
- Radiation Therapy and Dosimetry
- Gastrointestinal Tumor Research and Treatment
- Skin and Cellular Biology Research
- Leptospirosis research and findings
- Colorectal and Anal Carcinomas
- Anesthesia and Neurotoxicity Research
- Cutaneous lymphoproliferative disorders research
- Clinical Nutrition and Gastroenterology
Cedars-Sinai Medical Center
2019-2025
VA Greater Los Angeles Healthcare System
2016-2025
Los Angeles Medical Center
2023-2024
University of Kansas Medical Center
2024
Clark Atlanta University
2023
VA West Los Angeles Medical Center
2020-2023
Center for Cancer Research
2023
University of California, Los Angeles
2011-2022
West Los Angeles College
2013-2021
University of Vermont Medical Center
2013-2021
Interference with cerebral energy metabolism due to excess ammonia has been postulated as a cause of hepatic encephalopathy. Furthermore, consideration the neurologic basis such features encephalopathy asterixis, decerebrate rigidity, hyperpnea, and coma suggests malfunction structures in base brain their cortical connections. The three major sources intracerebral energy, adenosine triphosphate (ATP), phosphocreatine, glucose, well glycogen, were assayed cortex rats given ammonium acetate...
The intestinal crypt-niche interaction is thought to be essential the function, maintenance, and proliferation of progenitor stem cells found at bases crypts. These are constantly renewing epithelium by sending differentiated from base crypts Lieberkühn villus tips where they slough off into lumen. niche consists various cell types, extracellular matrix, growth factors surrounds cells. There have recently been advances in understanding interactions that regulate behavior there great interest...
Background We previously reported in vitro maintenance and proliferation of human small intestinal epithelium using Matrigel, a proprietary basement membrane product. There are concerns over the applicability Matrigel-based methods for future therapies. investigated type I collagen as an alternative culture epithelial cells. Methods Human intestine was procured from fresh surgical pathology specimens. Small crypts were isolated EDTA chelation. Intestinal subepithelial myofibroblasts...
The transcriptional co-activator Yes-associated protein 1 (YAP1), a key nuclear effector of the Hippo pathway, is potent oncogene, and yet, interaction between YAP1 androgen receptor (AR) remains unexplored. Here we identify as physiological binding partner positive regulator AR in prostate cancer. co-localize interact with each other predominantly within cell nuclei by an androgen-dependent mechanism hormone naive androgen-independent castration-resistant cancer cells. growth suppressor...
Intestinal epithelial stem cells (ISCs) are the focus of recent intense study. Current in vitro models rely on supplementation with Wnt agonist R-spondin1 to support robust growth, ISC self-renewal, and differentiation. subepithelial myofibroblasts (ISEMFs) important supportive within niche. We hypothesized that co-culture ISEMF enhances growth ISCs allows for their successful vivo implantation engraftment. ISC-containing small intestinal crypts, FACS-sorted single ISCs, ISEMFs were procured...
Background & Aims Intestinal microfold (M) cells are specialized epithelial that act as gatekeepers of luminal antigens in the intestinal tract. They play a critical role mucosal immune response through transport viruses, bacteria and other particles across epithelium to within Peyer's patch regions sites. Recent studies mice have demonstrated M generated from Lgr5+ stem (ISCs), infection with Salmonella enterica serovar Typhimurium increases cell formation. However, it is not known whether...
Pancreatic ductal adenocarcinoma (PDAC) has a five-year survival rate of less than 10% due to its late diagnosis, rapid metastasis, and chemotherapeutic resistance. For small proportion (10-20%) early-stage patients however, surgical resection the pancreatic tumor offers best chance for but effect surgery on disease dissemination is unknown. The primary objective this study was characterize cellular acellular blood-based analytes in portal peripheral blood before manipulation, during...
<h3>Background</h3> A defining characteristic of the human intestinal epithelium is that it most rapidly renewing tissue in body. However, processes underlying renewal and mechanisms govern their coordination have proved difficult to study gut. <h3>Objective</h3> To investigate regulation stem cell-driven by canonical Wnt TGFβ/bone morphogenetic protein (BMP) pathways native colonic epithelium. <h3>Design</h3> Intact crypts were isolated from mucosal samples placed into 3D culture conditions...
The leptospiral LigA protein consists of 13 bacterial immunoglobulin-like (Big) domains and is the only purified recombinant subunit vaccine that has been demonstrated to protect against lethal challenge by a clinical isolate Leptospira interrogans in hamster model leptospirosis. We determined minimum number location required for immunoprotection. Immunization with 11 12 was found be but insufficient protection. Inclusion third domain, either 10 or 13, 100% survival after intraperitoneal...
Methods for the in vitro culture of primary small intestinal epithelium have improved greatly recent years. A critical barrier translation this methodology to patient's bedside is ability grow stem cells using a well-defined extracellular matrix. Current methods rely on use Matrigel(™), proprietary basement membrane-enriched matrix gel produced mice that not approved clinical use. We demonstrate first time capacity support long-term growth murine monoculture, type I collagen. further...
The Seraph100 Microbind Affinity Blood Filter (Seraph 100) (ExThera Medical, Martinez, CA) is an extracorporeal therapy that can remove pathogens from blood, including severe acute respiratory syndrome coronavirus 2. aim of this study was to evaluate safety and efficacy Seraph 100 treatment for COVID-19.Retrospective cohort study.Nine participating ICUs.COVID-19 patients treated with (n = 53) control matched by site 53).Treatment 100.At baseline, there were no differences between the groups...
Abstract Background Keratins (KRTs) are intermediate filament proteins that interact with multiple regulatory to initiate signaling cascades. Keratin 13 (KRT13) plays an important role in breast cancer progression and metastasis. The objective of this study is elucidate the mechanism by which KRT13 promotes growth Methods function mechanisms metastasis were assessed overexpression knockdown followed examination altered behaviors cells xenograft tumor formation mouse mammary fat pad. Human...
The capacity of the intestine to secrete fluid is dependent on basolateral Na + –K –2Cl − co‐transporter (NKCC1). Given that cAMP and Ca 2 signals promote sustained transient episodes secretion, respectively, this study investigated differential regulation functional NKCC1 membrane expression in native human colonic epithelium. Tissue sections crypts were obtained from sigmoid rectal biopsy tissue samples. Cellular location NKCC1, ‐ATPase, M3 muscarinic acetylcholine receptor (M 3 AChR)...
Engineering the osteochondral junction requires fabrication of a microenvironment that supports both osteogenesis and chondrogenesis. Multiphasic scaffold strategies utilizing combination soluble factors extracellular matrix components are ideally suited for such applications. In this work, contribution an osteogenic nanoparticulate mineralized glycosaminoglycan (MC‐GAG) dually chondrogenic growth factor, BMP‐9, in differentiation primary human mesenchymal stem cells (hMSCs) is evaluated....
Background Leptospirosis is a zoonosis caused by highly motile, helically shaped bacteria that penetrate the skin and mucous membranes through lesions or abrasions, rapidly disseminate throughout body. Although intraperitoneal route of infection widely used to experimentally inoculate hamsters, this challenge does not represent natural infection. Methodology/Principal Findings Here we describe kinetics disease in hamster model leptospirosis after subcutaneous intradermal inoculation...
Cancer becomes lethal as it spreads from the primary site to rest of body. Circulating tumor cells (CTCs) are biomarkers disease progression and have been associated with decreased overall survival. Blood filtration is a novel concept for removing CTCs circulation improve patient prognosis.