A5018712181- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Periodontal Regeneration and Treatments
- Cellular Mechanics and Interactions
- Tendon Structure and Treatment
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Collagen: Extraction and Characterization
- Tissue Engineering and Regenerative Medicine
- Proteoglycans and glycosaminoglycans research
- Electrospun Nanofibers in Biomedical Applications
- Bone and Dental Protein Studies
- Reproductive System and Pregnancy
- Cancer, Hypoxia, and Metabolism
- Shoulder Injury and Treatment
- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- Nanofabrication and Lithography Techniques
- Bone Metabolism and Diseases
- Bone fractures and treatments
- Immune cells in cancer
- Facial Trauma and Fracture Management
- Angiogenesis and VEGF in Cancer
University of Illinois Urbana-Champaign
2016-2025
Center for Genomic Science
2014-2024
Urbana University
2014-2022
Creative Commons
2016-2022
University of California, Los Angeles
2018-2021
NOAA Chemical Sciences Laboratory
2020
UCLA Medical Center
2017
Boston Children's Hospital
2007-2012
Harvard University
2012
Massachusetts Institute of Technology
2004-2010
The permeability of scaffolds and other three-dimensional constructs used for tissue engineering applications is important as it controls the diffusion nutrients in waste out scaffold well influencing pressure fields within construct. objective this study was to characterize permeability/fluid mobility collagen-GAG a function pore size compressive strain using both experimental mathematical modeling techniques. Scaffolds containing four distinct mean sizes (151, 121, 110, 96 microns) were...
Adult mesenchymal stem cells have the proclivity to differentiate along multiple lineages giving rise new bone, cartilage, muscle, or fat. Collagen, a normal constituent of provides strength and structural stability is therefore potential candidate for use as substrate on which engineer bone cartilage from their respective mesenchymal-derived precursors. In this study, collagen- glycosaminoglycan scaffold was used provide suitable three-dimensional (3-D) environment culture adult rat induce...
Abstract There is a need to improve current treatments for articular cartilage injuries. This article the third in series describing design and development of an osteochondral scaffold based on collagen‐glycosaminoglycan calcium phosphate technologies regenerative repair defects. The previous articles this described methods producing porous, three‐dimensional mineralized collagen‐GAG (CGCaP) scaffolds whose composition can be reproducibly varied mimic subchondral bone, pore microstructure...
Abstract This paper is the second in a series of papers describing design and development an osteochondral scaffold using collagen–glycosaminoglycan calcium phosphate technologies engineered for regenerative repair articular cartilage defects. The previous described technology (concurrent mapping) systematic variation control chemical composition triple coprecipitated collagen, glycosaminoglycan, (CGCaP) nanocomposites without titrants. describes (1) fabricating porous, three‐dimensional...
Hematopoiesis is the physiological process where hematopoietic stem cells (HSCs) continuously generate body's complement of blood and immune within unique regions bone marrow termed niches. Although previous investigations have revealed gradients in cellular extracellular matrix (ECM) content across marrow, elasticity ligand type are believed to be strong regulators cell fate, impact biophysical signals on HSC response poorly understood. Using marrow-inspired ECM ligand-coated polyacrylamide...
Three‐dimensional tissue engineered constructs provide a platform to examine how the local extracellular matrix (ECM) contributes malignancy of cancers such as human glioblastoma multiforme. Improved resolution biophysical features impact glioma proliferation, genomic and signal transduction paths, well phenotypic markers would complement recent improvements in our understanding molecular mechanisms associated with enhanced malignancy. Here, we report use gelatin methacrylate (GelMA) create...
There is an acute need for biomaterial tools that recreate the heterogeneous brain-tumor microenvironment. A microfluidic mixing tool reported to encapsulate glioblastoma multiforme cells within miniaturized gelatin hydrogels containing overlapping patterns of tumor-inspired matrix signals. This approach permits in situ analysis glioma at molecular and genomic level as well potential clinical insight.
The bone marrow provides spatially and temporally variable signals that impact the behavior of hematopoietic stem cells (HSCs). While multiple biomolecular cell populations have been proposed as key regulators HSC fate, new tools are required to probe their importance mechanisms action. Here, a novel method based on microfluidic mixing platform create small volume, 3D hydrogel constructs containing overlapping patterns matrix constituents inspired by niche is described. This approach used...
Biomaterial approaches for engineering orthopedic interfaces such as the tendon‐bone junction (TBJ) are limited by a lack of understanding how insoluble (microstructure, composition) and soluble regulators stem cell fate work in concert to promote bioactivity differentiation. One strategy regenerating interface is design biomaterials containing spatially graded structural properties sufficient induce divergent mesenchymal (MSC) differentiation into multiple interface‐specific phenotypes....
Glioblastoma (GBM) is the most common and lethal form of brain cancer. Its high mortality associated with its aggressive invasion throughout brain. The heterogeneity stiffness hyaluronic acid (HA) content within makes it difficult to study in vivo. A dextran‐bead assay employed quantify GBM HA‐functionalized gelatin hydrogels. Using a library stiffness‐matched hydrogels variable levels matrix‐bound HA, reported that U251 enhanced softer but reduced presence HA. Inhibiting HA–CD44...
The extracellular matrix (ECM) is critical in tumor growth and invasive potential of cancer cells. In glioblastoma tumors, some components the native brain ECM such as hyaluronic acid (HA) have been suggested key regulators processes associated with poor patient outlook invasion therapeutic resistance. Given importance cell-mediated remodeling during invasion, it likely that molecular weight available HA polymer may strongly influence GBM progression. Biomaterial platforms therefore provide...