Cellular processes are intrinsically complex and dynamic, in which a myriad of cellular components including nucleic acids, proteins, membranes, organelles involved undergo spatiotemporal changes. Label-free Raman imaging has proven powerful for studying such dynamic behaviors vivo at the molecular level. To construct images, univariate data analysis been commonly employed, but it cannot be free from uncertainties due to severely overlapped spectral information. Here, we demonstrate...

Abstract Pink biofilms are multispecies microbial communities that commonly found in moist household environments. The development of this pink stain is problematic from an aesthetic point view, but more importantly, it raises hygienic concerns because they may serve as a potential reservoir opportunistic pathogens. Although there have been several studies using molecular analysis and confocal laser scanning microscopy, little known about the spatial distributions constituent microorganisms...

ABSTRACT Bacterial discrimination using single‐cell Raman spectroscopy and machine/deep learning techniques has been widely explored for promising applications in medical, environmental, food sciences. To construct a machine‐learning model that can achieve highly accurate robust of bacteria real‐world samples, data consisting spectra bacterial cells acquired under various physiological conditions are essential. Despite much effort to study the effects growth phase on discrimination, it is...

In vivo time-lapse Raman imaging reveals highly dynamic and concerted changes in concentration distribution of phospholipids proteins during after cell division a single living Schizosaccharomyces pombecell.

Abstract In vivo , molecular-level investigation of cytokinesis, the climax cell cycle, not only deepens our understanding how life continues, but it will also open up new possibilities diagnosis/prognosis cancer cells. Although fluorescence-based methods have been widely employed to address this challenge, they require a fluorophore be designed for specific known biomolecule and introduced into cell. Here, we present label-free spectral imaging approach based on multivariate curve...

Understanding cellular metabolism is a major challenge in current systems biology and has triggered extensive metabolomics research, which most cases involves destructive analysis. However, the information obtainable only nondestructive manner will be required for accurately mapping global structure of organism's metabolic network at given instant. Here we report that pathways can explored vivo by mixed stable isotope-labeled Raman microspectroscopy conjunction with multivariate curve...

Anti-Stokes Raman scattering is used to monitor vibrational energy redistribution in the ambient temperature liquids nitromethane (NM-h3) and perdeuterated (NM-d3) after ultrafast IR excitation of either symmetric or asymmetric CH- CD-stretch transitions. The instantaneous populations most fifteen NM vibrations are determined with good accuracy, a global fitting procedure master equation fit all data. pump pulses excite not only CD-stretches but also certain combinations bending nitro...

Aggregation of bacterial populations substantially influences their characteristic properties and functions compared with the planktonic counterpart. It is also involved in initial stages biofilm development. Many studies have revealed important roles aggregation microbial production biodegradation. Nevertheless, mechanistic understanding vivo at molecular level far from complete. Here, we present a noninvasive, label-free Raman microspectroscopic approach to investigate development...

Abstract In structured communities of bacteria known as biofilms, a variety biomolecules have been shown to play unique role signals and/or regulators in biofilm formation. Here, we report that high levels the amino acid leucine (leucine pool) were detected, for first time, within microcolonies 30‐h‐old Escherichia coli by Raman imaging. Localization revealed multifrequency images indicates accumulation during early stage E. formation, which may resulted from physiological...

Solar cells based on organometal-halide perovskites such as CH3NH3PbI3 have emerged a promising next-generation photovoltaic system, but the underlying photophysics and photochemistry remain to be established because of limited availability methods implement simultaneous direct measurement various charge carriers ions that play crucial role in operating device. We used nanosecond time-resolved infrared (IR) spectroscopy investigate, with high molecular specificity, distinct transient species...

Transient absorption spectra of 9,9′-bianthryl (BA) in heptane, acetonitrile, and 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (bmimTf2N) are observed with a nanosecond time-resolved near-IR spectrometer for the wavenumber range 4500−10 500 cm−1 (2200−950 nm). In nonpolar heptane solution, broad band is at 6700 (1500 nm), addition to strong locally excited (LE) state centered 9800 (1020 The assigned partial charge transfer (PCT) band. decay time constants PCT LE both (13 ±...

Hyphae of filamentous fungi consist compartments that are distinct both spatially and functionally, thereby forming a unique multicellular system. Much work has been done mainly using fluorescence imaging to reveal what biomolecules present in those different hyphal sections physiological roles they play. Nevertheless, holistic understanding functions including the polarized growth hyphae is still lacking because difficulty simultaneous acquisition spatial chemical information on various...

The effects of interionic hydrogen bonding and π-π stacking interactions on the physical properties a new series picrate anion based ionic liquids (ILs) have been investigated experimentally theoretically. existence aromatic (C2-HO) aliphatic (C7-HO-N22 C6-HO-N20) in these ILs has observed using various spectroscopic techniques. C-HO are found to crucial role binding imidazolium cation together. However, between two successive layers play decisive tight packing leading differences...

Extremely low-frequency (LF) Raman spectroscopy that allows for fast access to lattice vibrations with little hindrance of immense Rayleigh scattering was used track in situ thermally induced polymorphic transformation crystalline 1,1′-binaphthyl (BN), a fundamental structural unit specific chiral ligands. BN occurs two conformational polymorphs, cisoid and transoid forms, the former being racemate latter conglomerate crystals. Observed LF spectra (from 200 cm–1 down ∼5 cm–1, equivalent 0.15...

Filamentous fungi grow exclusively at their tips, where many growth-related fungal processes, such as enzyme secretion and invasion into host cells, take place. Hyphal tips are also a site of active metabolism. Understanding metabolic dynamics within the tip region is therefore important for biotechnology medicine well microbiology ecology. However, methods that can track with sufficient spatial resolution in nondestructive manner highly limited. Here we present time-lapse Raman imaging...

Ultrafast infrared-Raman spectroscopy is used to study vibrational energy dynamics of three molecules in aqueous solution (D2O) that serve as models for the building blocks peptides. These are glycine-d3 zwitterion (GLY), N-methylacetamide-d (NMA), and benzoate anion (BZ). GLY simplest amino acid, NMA a model compound with peptide bond, BZ aromatic side chains. An ultrashort IR pulse pumps parent CH-stretch on each solute. Anti-Stokes Raman monitors flow through solutes' strongly...

Water in nanoconfinement shows distinct properties that are markedly different from those of bulk water. These unique stem not only the water-water interaction but also interactions between water and surrounding confining environment. Here we used a combined approach vibrational spectroscopies (Raman, FTIR, IR electroabsorption) multivariate curve resolution technique to study heterogeneous environment within prototype pillared layer-type metal-organic frameworks (MOFs), CPL-1...

Combination bands that involve CH- or OH-stretch vibrations appear in the near-infrared (NIR) region (4000–10 000 cm−1). Because they arise from anharmonic coupling between component fundamentals, detailed analysis of frequency and intensity NIR combination allows one to elucidate mechanisms behind vibrational condensed phase terms mechanical electrical anharmonicities. Nevertheless, little has been studied, particular experimentally, on origin band intensity. Here, we show electroabsorption...

Abstract Back electron transfer (BET) is one of the important processes that govern decay generated ion pairs in intermolecular photoinduced reactions. Unfortunately, a detailed mechanism BET reactions remains largely unknown spite their importance for development molecular photovoltaic structures. Here, we examine reaction pyrene (Py) and 1,4‐dicyanobenzene (DCB) acetonitrile (ACN) by using time‐resolved near‐ mid‐IR spectroscopy. The Py dimer radical cation (Py 2 .+ ) DCB anion (DCB .−...