A5078611949- Platelet Disorders and Treatments
- Extracellular vesicles in disease
- Venous Thromboembolism Diagnosis and Management
- Hemophilia Treatment and Research
- Blood groups and transfusion
- Heparin-Induced Thrombocytopenia and Thrombosis
- Blood transfusion and management
- Blood Coagulation and Thrombosis Mechanisms
- Liver Disease Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Liver Disease and Transplantation
- Inflammatory Biomarkers in Disease Prognosis
- Blood properties and coagulation
- Blood donation and transfusion practices
- Intramuscular injections and effects
- Sepsis Diagnosis and Treatment
- Nanopore and Nanochannel Transport Studies
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Cerebral Venous Sinus Thrombosis
- Erythrocyte Function and Pathophysiology
- Characterization and Applications of Magnetic Nanoparticles
- Solar-Powered Water Purification Methods
- Flow Measurement and Analysis
- Proteoglycans and glycosaminoglycans research
Centre Hospitalier Universitaire de Besançon
2018-2024
Université de franche-comté
2013-2024
Établissement Français du Sang
2013-2022
Inserm
2012-2021
Université Bourgogne Franche-Comté
2015-2021
Coronavirus disease 2019 (COVID-19) may predispose to venous thromboembolism. We determined factors independently associated with computed tomography pulmonary angiography (CTPA)-confirmed embolism (PE) in hospitalised severe COVID-19 patients.Among all (n=349) patients for a university hospital French region high rate of COVID-19, we analysed who underwent CTPA clinical signs (oxygen saturation measured by pulse oximetry ≤93% or breathing ≥30 breaths·min-1) rapid worsening. Multivariable...
Abstract Extracellular vesicles (EVs) are active components of red blood cell (RBC) concentrates and may be associated with beneficial adverse effects transfusion. Elucidating controllable factors EV release in RBC products is thus important to better manage the quality properties units. Erythrocyte-derived EVs (EEVs) platelet-derived (PEVs) were counted 1226 units (administered 280 patients) using a standardized cytometry-based method. size CD47 annexin V expression also measured. The donor...
Summary The ristocetin cofactor activity assay (VWF:RCo) is the reference method for assessing von Willebrand factor (VWF) but remains difficult to perform, and coefficient of variation high (about 20–30%). This study evaluated compared performance measuring VWF two newly commercialised assays [VWF:Ac Innovance (VWF:Ac) VWF:RCo Acustar (VWF:RCo Acu)] with aggregation in 123 pathological plasma samples. correlation concordance between both new tests (VWF:RCo-Acu VWF:Ac) were good. results...
Introduction: Cerebral venous thrombosis (CVT) is a rare disease with highly variable clinical presentation and outcome. Etiological assessment may be negative. The radiological evolution can variable. mechanisms involved in this variability remain unknown. Objective: aim of multicenter French study registered on ClinicalTrials.gov (NCT02013635) was therefore to prospectively recruit cohort patients cerebral (FPCCVT) order thrombin generation clot degradation, evaluate their influence...
BACKGROUND The procoagulant and proinflammatory microparticles (MPs) released during storage of packed red blood cells (pRBCs) can potentially modify transfusion benefits. A robust method to quantify MPs in pRBCs is needed evaluate their impact clinical trials. STUDY DESIGN AND METHODS objective was validate the preanalytic conditions required prepare pRBC supernatant as well a MP variations over 42 days storage.A flow cytometry with size‐calibrated beads developed fully validated....
Immune thrombocytopenia (ITP) is a rare autoimmune disease due to autoantibodies targeting platelet glycoproteins (GP). The mechanism of destruction could differ depending on the specificity antiplatelet antibodies: anti-GPIIb/IIIa antibodies lead phagocytosis by splenic macrophages, in Fcγ receptor (FcγR)-dependent manner while anti-GPIb/IX induce desialylation leading their hepatocytes after binding Ashwell-Morell receptor, FcγR-independent manner. Considering FcγR-dependent action...
The quantification of lipopolysaccharide (LPS) in biological fluids is challenging. We aimed to measure plasma LPS concentration using a new method direct 3-hydroxymyristate (3-HM), lipid component LPS, and evaluate correlations between 3-HM markers liver function, endothelial activation, portal hypertension enterocyte damage.Plasma from 90 noninfected cirrhotic patients (30 Child-Pugh [CP]-A, 30 CP-B, CP-C) was prospectively collected. determined by high-performance liquid chromatography...
Microvesicles (MVs) with procoagulant properties may favor liver parenchymal extinction, then cirrhosis-related complications and mortality. In a longitudinal cohort of cirrhotic patients, we measured plasma levels platelet-derived MVs (PMVs), endothelial-derived MVs, red blood cell-derived expressing phosphatidylserine (annexin V-positive [AV+]) or not, evaluated their impact on Model for End-Stage Liver Disease (MELD) score transplant-free survival.MVs were quantified using flow cytometry...
Introduction Severe haemophilia is a rare disease characterised by spontaneous bleeding from early childhood, which may lead to various complications, especially in joints. It nowadays possible avoid these complications thanks substitutive therapies for the issue of adherence major. The transition adolescence adulthood young people with severe critical period as it associated high risk lack healthcare, might have serious consequences on daily activities and quality life. Methods analysis We...
Turbidity analysis is widely used as a quantitative technique in hereditary dysfibrinogenemia. We aimed to compare several coagulation triggers dysfibrinogenemia and control plasmas. included 20 patients with dysfibrinogenemia, 19 hotspot mutations Aα Arg35His ( n = 9), Arg35Cys 2), γ Arg301His 6), Arg301Cys one Phe27Tyr, commercial pooled normal plasma. Fibrin polymerization was activated by bovine or human thrombin tissue factor (TF), the presence absence of type plasminogen activator. The...
Small batch-pooled (mini-pool) whole blood (WB)-derived plasma could be an alternative cost-effective source of therapeutic (TP), but carries increased risk transfusion-transmitted infection due to exposure the recipient several donors. This can mitigated by inactivation pathogens susceptible amotosalen-UVA (AUVA)-treatment. We evaluated conservation coagulation factors in AUVA-plasma prepared from WB stored overnight under routine operating conditions, determine its efficacy. Thrombin...