A5101970800- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Heart Failure Treatment and Management
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Ion channel regulation and function
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Circadian rhythm and melatonin
- Neurotransmitter Receptor Influence on Behavior
- Apelin-related biomedical research
- Estrogen and related hormone effects
- Growth Hormone and Insulin-like Growth Factors
- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- X-ray Diffraction in Crystallography
- Neuroscience of respiration and sleep
- Crystallization and Solubility Studies
- Neurological Disorders and Treatments
- Mast cells and histamine
- Pituitary Gland Disorders and Treatments
- Chemical Synthesis and Analysis
University of Geneva
2019
Georgetown University
1983-2017
Georgetown University Medical Center
2014-2017
National Institute of Mental Health
2003-2013
National Institutes of Health
2003-2013
National Research and Development Institute on Occupational Safety
2012
Universidad Nacional de Córdoba
2008
Sociedad Española de Medicina Interna
2007
University of Bern
2004-2006
United States Department of Health and Human Services
2003-2006
Background and Purpose —Angiotensin II, through stimulation of AT 1 receptors, not only controls blood pressure but also modulates cerebrovascular flow. We sought to determine whether selective antagonists could be therapeutically advantageous in brain ischemia during chronic hypertension. Methods —We pretreated spontaneously hypertensive rats (SHR) normotensive Wistar-Kyoto with the antagonist candesartan (CV-11974), 0.5 mg/kg per day, for 3 14 days, via subcutaneously implanted osmotic...
Pretreatment with angiotensin II AT(1) receptor antagonists protects against cerebral ischemia. We studied whether modulation of blood flow (CBF) and morphometric changes in brain arteries participated this protective mechanism.We pretreated adult spontaneously hypertensive rats equally antihypertensive doses candesartan (0.1 or 0.3 mg/kg per day), nicardipine captopril (3.0 day) for 3 28 days via subcutaneous osmotic minipumps followed by permanent left middle artery (MCA) occlusion distal...
The spontaneously hypertensive rat (SHR) is vulnerable to brain ischemia and stress exhibits a chronically stimulated angiotensin II system, cerebrovascular hypertrophy, inflammation. Pretreatment with type 1 (AT1) receptor antagonists protects from prevents the development of stress-induced gastric ulcers in part by reducing inflammation mucosa. We studied whether AT1 could exert antiinflammatory effects vasculature as mechanism for their protective against ischemia.Ten-week-old SHR...
Angiotensin II, which stimulates AT(1) receptors, is a brain and peripheral stress hormone. We pretreated rats with the receptor antagonist candesartan for 13 d via sc-implanted osmotic minipumps, followed by 24-h isolation in individual metabolic cages. measured angiotensin II receptor-type binding mRNAs tyrosine hydroxylase mRNA quantitative autoradiography situ hybridization, catecholamines HPLC, hormones RIA. Isolation increased hypothalamic paraventricular nucleus as well anterior...
Quantitative autoradiography revealed large numbers of angiotensin-II (AT) receptors in the 18-day-old rat embryo. The selective AT-1 antagonist DuP 753 readily competed for AT liver, lung parenchyma, and choroid plexus, these are classified as receptors. AT-2 displacers CGP 42112 A and/or PD 123177 with high affinity bound to most located skeletal muscle, skin, diaphragm, bronchi, stomach, amount fetal tissue was more than 10-fold higher that In muscle presence 10(-7) M A, indicating small...
Angiotensin II is a vasoactive peptide and may act as growth factor in vascular smooth muscle cells. Experimental injury of the rat aorta causes rapid migration medial cells their proliferation resulting formation neointima. We have examined, using quantitative autoradiography, expression angiotensin receptor subtypes AT1 AT2, angiotensin-converting enzyme, neointima formed thoracic 15 d after balloon-catheter injury. In contrast to normal aortic wall, which contained both AT2 receptors (80%...
We have studied the properties of angiotensin II binding sites in paraventricular nucleus, subfornical organ and anterior pituitary lobe rats subjected to repeated immobilization stress. This treatment produced significant increase density these two nuclei without any alteration affinity. Repeated stress did not alter lobe. Our results suggest that brain may a role regulation response.
AT 2 receptors may act in opposition to and balance with 1 receptors, their stimulation having beneficial effects. We found renal receptor expression female mice higher than male mice. asked the question of whether such might be estrogen dependent. In male, female, ovariectomized, estrogen-treated ovariectomized mice, we studied by immunocytochemistry autoradiography, mRNA RT-PCR, cAMP, cGMP, PGE RIA. predominated. were present glomeruli, medullary rays, inner medulla, kidney capsule....
Stress reduces gastric blood flow and produces acute mucosal lesions. We studied the role of angiotensin II in ulceration during stress. Spontaneously hypertensive rats were pretreated for 14 days with AT1 receptor antagonist candesartan before cold-restraint receptors localized endothelium arteries mucosa all layers. blockade increased by 40-50%, prevented ulcer formation 70-80% after stress, reduced increase adrenomedullary epinephrine tyrosine hydroxylase mRNA without preventing...