A5004601748- Asthma and respiratory diseases
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Allergic Rhinitis and Sensitization
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Immune cells in cancer
- Organ Transplantation Techniques and Outcomes
- Eosinophilic Esophagitis
- Cardiac Ischemia and Reperfusion
- Congenital Anomalies and Fetal Surgery
- Cardiovascular Function and Risk Factors
- Cardiac Valve Diseases and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Electrostatic Discharge in Electronics
- High Altitude and Hypoxia
- Abdominal vascular conditions and treatments
- Cholesterol and Lipid Metabolism
- Invertebrate Immune Response Mechanisms
- Pluripotent Stem Cells Research
- Mast cells and histamine
- Respiratory viral infections research
- Immunotherapy and Immune Responses
National Institutes of Health
2010-2024
National Heart Lung and Blood Institute
2010-2024
Institute of Laboratory Animal Science
2010-2012
Rationale: Distinct sets of corticosteroid-unresponsive genes modulate disease severity in asthma.Objectives: To identify that provide new insights into pathogenesis and asthma therapeutics.Methods: Experimental murine was induced by nasal administration house dust mite for 5 days per week. Dexamethasone apolipoprotein E (apo E) mimetic peptides were administered via osmotic minipumps.Measurements Main Results: Genome-wide expression profiling the lung transcriptome a mite–induced model...
Abstract New treatment approaches are needed for patients with asthma. Apolipoprotein A-I (apoA-I), the major structural protein of high-density lipoproteins, mediates reverse cholesterol transport and has atheroprotective anti-inflammatory effects. In this study, we hypothesized that an apoA-I mimetic peptide might be effective at inhibiting asthmatic airway inflammation. A 5A peptide, which is a synthetic, bihelical mimetic, was administered to wild-type A/J mice via osmotic mini-pump...
Section:ChooseTop of pageAbstract <<Marterials and MethodsResultsDiscussionReferencesCITING ARTICLES
DNA-dependent protein kinase (DNA-PK) mediates double-stranded DNA break repair, V(D)J recombination and immunoglobulin class switch recombination, as well innate immune pro-inflammatory responses. However, there is limited information regarding the role of DNA-PK in adaptive immunity mediated by dendritic cells (DCs), which are primary antigen-presenting allergic asthma. Here we show that house dust mite induces phosphorylation, a marker activation, DCs via generation intracellular reactive...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
The mammalian target of rapamycin (mTOR) modulates immune responses and cellular proliferation. objective this study was to assess whether inhibition mTOR with modifies disease severity in two experimental murine models house dust mite (HDM)-induced asthma. In an induction model, administered BALB/c mice coincident nasal HDM challenges for 3 weeks. a treatment were performed 6 weeks during 4 through 6. the significantly attenuated airway inflammation, hyperreactivity (AHR) goblet cell...
Peptidoglycan recognition protein (Pglyrp) 1 is a pattern-recognition that mediates antibacterial host defense. Because we had previously shown Pglyrp1 expression increased in the lungs of house dust mite (HDM)-challenged mice, hypothesized it might modulate pathogenesis asthma. Wild-type and Pglyrp1(-/-) mice on BALB/c background received intranasal HDM or saline, 5 days/week for 3 weeks. HDM-challenged showed decreases bronchoalveolar lavage fluid eosinophils lymphocytes, serum IgE, mucous...
Apolipoprotein E (apoE) is an endogenous negative regulator of airway hyperreactivity (AHR) and mucous cell metaplasia in experimental models house dust mite (HDM)-induced disease. The gene encoding human apoE polymorphic, with three common alleles (ε2, ε3, ε4) reflecting single amino acid substitutions at acids 112 158. objective this study was to assess whether the modify responses repeated nasal HDM challenges. Mice expressing ε2 (huApoE2), ε3 (huApoE3), or ε4 (huApoE4) received...
The very low density lipoprotein receptor (VLDLR) is a member of the low-density family that binds multiple ligands and plays key role in brain development. Although VLDLR mediates pleiotropic biological processes, only limited amount information available regarding its adaptive immunity. In this study, we identify an important for attenuating house dust mite (HDM)-induced airway inflammation experimental murine asthma. We show HDM-challenged Vldlr(-/-) mice have augmented eosinophilic...
Cardiovascular disease remains the number one cause of mortality in United States. There are numerous approaches to treating these diseases, but regardless approach, an vivo model is needed test each treatment. The pig most used large animal models for cardiovascular disease. Its heart very similar anatomy and function that a human. ameroid placement technique creates ischemic area heart, which has many useful applications studying myocardial infarction. This been surgical research,...
Cardiovascular disease remains the number one cause of mortality in United States. There are numerous approaches to treating these diseases, but regardless approach, an vivo model is needed test each treatment. The pig most used large animal models for cardiovascular disease. Its heart very similar anatomy and function that a human. ameroid placement technique creates ischemic area heart, which has many useful applications studying myocardial infarction. This been surgical research,...
Prolyl hydroxylation is a post-translational modification that regulates protein stability, turnover, and activity. The proteins catalyze prolyl belong to the 2-oxoglutarate- iron-dependent oxygenase family of proteins. domain-containing 1 (Ogfod1), which hydroxylates proline in ribosomal s23 newly described member this family. aims study were investigate roles for Ogfod1 heart, heart's response stress.We isolated hearts from wild-type (WT) knockout (KO) mice performed quantitative...
Abstract Neutrophils play important roles in inflammatory airway diseases. In this study, we assessed whether apolipoprotein A-I modifies neutrophil heterogeneity as part of the mechanism by which it attenuates acute inflammation. Neutrophilic inflammation was induced daily intranasal administration LPS plus house dust mite (LPS+HDM) to Apoa1−/− and Apoa1+/+ mice for 3 d. Single-cell RNA sequencing performed on cells recovered from bronchoalveolar lavage fluid day 4. Unsupervised profiling...
Intestinal ischemia-reperfusion injury (IRI) is associated with a myriad of conditions in both veterinary and human medicine. IRI conditions, such as gastric dilatation volvulus (GDV), mesenteric torsion, colic, are observed animals dogs horses. An initial interruption blood flow causes tissues to become ischemic. Although necessary salvage viable tissue, subsequent reperfusion can induce further injury. The main mechanism responsible for free radical formation upon reintroduction oxygen...
Patients with ADP-ribose-acceptor hydrolase 3 ( ARH3 ) deficiency exhibit stress-induced childhood-onset neurodegeneration ataxia and seizures (CONDSIAS). degrades protein-linked poly(ADP- ribose) (PAR) synthesized by poly(ADP-ribose)polymerase (PARP)-1 during oxidative stress, leading to cleavage of the ADP-ribose linked protein. leads excess accumulation PAR, resulting in PAR-dependent cell death or parthanatos. Approximately one-third patients homozygous mutant die from cardiac arrest,...
Intestinal ischemia-reperfusion injury (IRI) is associated with a myriad of conditions in both veterinary and human medicine. IRI conditions, such as gastric dilatation volvulus (GDV), mesenteric torsion, colic, are observed animals dogs horses. An initial interruption blood flow causes tissues to become ischemic. Although necessary salvage viable tissue, subsequent reperfusion can induce further injury. The main mechanism responsible for free radical formation upon reintroduction oxygen...