A5046010386- Dermatological and Skeletal Disorders
- Cerebrovascular and genetic disorders
- Parathyroid Disorders and Treatments
- Cell Adhesion Molecules Research
- Ultrasound and Hyperthermia Applications
- Heterotopic Ossification and Related Conditions
- Photoacoustic and Ultrasonic Imaging
- Venous Thromboembolism Diagnosis and Management
- Moyamoya disease diagnosis and treatment
- Fluid Dynamics and Turbulent Flows
- Cerebrovascular and Carotid Artery Diseases
- Immune Cell Function and Interaction
- Nanofluid Flow and Heat Transfer
- Metalloenzymes and iron-sulfur proteins
- Pluripotent Stem Cells Research
- Connective tissue disorders research
- Atherosclerosis and Cardiovascular Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Radiopharmaceutical Chemistry and Applications
- Neurological diseases and metabolism
- Heat and Mass Transfer in Porous Media
- Cardiac Fibrosis and Remodeling
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Alkaline Phosphatase Research Studies
- Congenital heart defects research
National Heart Lung and Blood Institute
2017-2024
National Institutes of Health
2017-2024
Center for Neuroscience and Regenerative Medicine
2023
University of Virginia
2003-2016
HemoSonics (United States)
2016
Ospedale Sacro Cuore Don Calabria
2015
Salford Royal NHS Foundation Trust
2015
Danderyds sjukhus
2015
Cliniques Universitaires Saint-Luc
2015
The George Institute for Global Health
2015
Vascular aging impacts multiple organ systems, including the brain, where it can lead to vascular dementia. However, a concrete understanding of how specifically affects brain vasculature, along with molecular read-outs, remain vastly incomplete. Here we demonstrate that is associated marked decline in Notch3 signaling both murine and human vessels. To clarify consequences loss used single-cell transcriptomics uncovered inactivation alters regulation calcium, contractile function, promotes...
Major surgical procedures often result in significant intra- and postoperative bleeding. The ability to identify the cause of bleeding has potential reduce transfusion blood products improve patient care. We present a novel device, Quantra Hemostasis Analyzer, which been designed for automated, rapid, near-patient monitoring hemostasis. is based on Sonic Estimation Elasticity via Resonance Sonorheometry, proprietary technology that uses ultrasound measure clot time stiffness from changes...
Background: Arterial calcification due to deficiency of CD73 (ACDC; OMIM 211800) is a rare genetic disease resulting in calcium deposits arteries and small joints causing claudication, resting pain, severe joint deformities. Currently, there are no standard treatments for ACDC. Our previous work identified etidronate as potential targeted ACDC treatment, using vitro vivo models with patient-derived cells. In this study, we test the safety effectiveness attenuating progression lower-extremity...
To evaluate targeting of a microbubble contrast agent to platelets under high shear flow using the natural selectin ligand sialyl Lewis.
Arterial calcification due to deficiency of CD73 (ACDC) is a hereditary autosomal recessive ectopic mineralization syndrome caused by loss-of-function mutations in the ecto-5'-nucleotidase gene. Periarticular has been reported but clinical characterization arthritis as well microstructure and chemical composition periarticular calcifications SF crystals not systematically investigated.Eight ACDC patients underwent extensive rheumatological radiological evaluation over period 11 years....
Chemokine receptor 5 (CCR5) is the primary coreceptor for HIV entry into macrophages. Individuals with a homozygous deletion of 32 bp in CCR5 gene (CCR5Δ32) are highly resistant to infection (Samson et al., 1996). Allogeneic stem cell transplantation from healthy donor CCR5Δ32 variant an positive individual has demonstrated efficient long-term control HIV. We identified three individuals this variant, and successfully generated induced pluripotent (iPSC) lines their dermal fibroblasts. The...
Platelets can be found on the surface of inflamed and ruptured atherosclerotic plaques. Thus, targeting activated platelets may allow for molecular imaging vulnerable lesions. We here investigated microbubbles (MB) functionalized with selectin ligand sialyl Lewisa individually (MBsLea) or dually sLea an antibody ligand-induced binding sites GPIIb/IIIa receptor (MBDual). Assessed by in vitro flow chamber, targeted MB exhibited increased adhesion to as compared MBControl. While MBsLea rolled...
Human induced pluripotent stem cell (iPSC) technology has opened exciting opportunities for stem-cell-based therapy. However, its wide adoption is precluded by several challenges including low reprogramming efficiency and potential malignant transformation. Better understanding of the molecular mechanisms changes that cells undergo during reprograming needed to improve iPSCs generation increase confidence their clinical use safety. Here, we find dominant negative mutations in STAT3 patients...
A 54-year old female patient with the genetic disease of arterial calcification due to deficiency CD73 was studied under Undiagnosed Disease Program National Institutes Health. She presented symptoms claudication in her 40s and later developed arthritic symptoms, ectopic left hand severe calcifications lower extremities. Since little known about composition CD73, we investigated their chemical identity microscopic morphology this imaging x-ray diffraction analysis. We found that,...
Autosomal dominant Hyper IgE syndrome (AD-HIES), a rare immune deficiency affecting fewer than one per million people, is caused by heterozygous deleterious mutations in STAT3. STAT3 signaling plays crucial roles basic cellular functions broad aspects of homeostasis. Accordingly, addition to immunological deficits, patients experience severe multisystem non-immunological features. Human induced pluripotent stem cells (hiPSC) are well established as vivo disease models for various human...
We have successfully generated induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells of five patients with Cerebral autosomal-dominant arteriopathy subcortical infarcts and leukoencephalopathy (CADASIL). These carry the genetic NOTCH3 mutation present in their parental cells. iPSC exhibited normal karyotype phenotype, which were sustained through propagation. Furthermore, these iPSCs displayed capacity differentiating toward three germ layers vitro.