A5078337349- Cerebrovascular and genetic disorders
- Cell Adhesion Molecules Research
- Metalloenzymes and iron-sulfur proteins
- Moyamoya disease diagnosis and treatment
- Developmental Biology and Gene Regulation
- Protease and Inhibitor Mechanisms
- Barrier Structure and Function Studies
- Neurobiology and Insect Physiology Research
- Acute Ischemic Stroke Management
- Estrogen and related hormone effects
- Circadian rhythm and melatonin
- Neuroinflammation and Neurodegeneration Mechanisms
- Functional Brain Connectivity Studies
- Heart Rate Variability and Autonomic Control
- Intracerebral and Subarachnoid Hemorrhage Research
- Neurological diseases and metabolism
- Angiogenesis and VEGF in Cancer
- EEG and Brain-Computer Interfaces
- Trace Elements in Health
- Paranormal Experiences and Beliefs
- Cardiac Arrest and Resuscitation
- RNA modifications and cancer
- Olfactory and Sensory Function Studies
- Cancer-related molecular mechanisms research
- Receptor Mechanisms and Signaling
University of Michigan
2016-2025
VA Ann Arbor Healthcare System
2015-2025
Southeast University
2023
Michael & Associates
2019-2023
Michigan United
2011-2020
Michigan Medicine
2013-2019
Neurology, Inc
1999-2015
Cardiovascular Research Center
2015
Nanjing Medical University
2013
Jiangsu Province Hospital
2013
The brain is assumed to be hypoactive during cardiac arrest. However, the neurophysiological state of immediately following arrest has not been systematically investigated. In this study, we performed continuous electroencephalography in rats undergoing experimental and analyzed changes power density, coherence, directed connectivity, cross-frequency coupling. We identified a transient surge synchronous gamma oscillations that occurred within first 30 s after preceded isoelectric...
N,N-dimethyltryptamine (DMT), a psychedelic compound identified endogenously in mammals, is biosynthesized by aromatic-L-amino acid decarboxylase (AADC) and indolethylamine-N-methyltransferase (INMT). Whether DMT the mammalian brain unknown. We investigated expression of INMT transcript rats humans, co-expression AADC mRNA rat periphery, concentrations rats. transcripts were cerebral cortex, pineal gland, choroid plexus both humans via situ hybridization. Notably, was colocalized with...
The brain is assumed to be hypoactive during cardiac arrest. However, animal models of and respiratory arrest demonstrate a surge gamma oscillations functional connectivity. To investigate whether these preclinical findings translate humans, we analyzed electroencephalogram electrocardiogram signals in four comatose dying patients before after the withdrawal ventilatory support. Two exhibited rapid marked power, cross-frequency coupling waves with slower oscillations, increased...
Vascular aging impacts multiple organ systems, including the brain, where it can lead to vascular dementia. However, a concrete understanding of how specifically affects brain vasculature, along with molecular read-outs, remain vastly incomplete. Here we demonstrate that is associated marked decline in Notch3 signaling both murine and human vessels. To clarify consequences loss used single-cell transcriptomics uncovered inactivation alters regulation calcium, contractile function, promotes...
Abstract Female patients experience substantial neuroprotection after experimental stroke compared with male patients, a finding attributed to the protective effects of gonadal hormones. This study examined response male‐ and female‐derived organotypic hippocampal slices oxidative excitotoxic injury. Both oxygen glucose deprivation N ‐methyl‐ D ‐aspartic acid exposure led neuronal death; however, cultures sustained less injury than male‐derived cultures. Cell death was ameliorated in...
mineralocorticoid receptor (MR) antagonists have protective effects in rodent models of ischemic stroke, but the cell type-specific actions these drugs are unknown. In present study, we examined contribution myeloid MR during focal cerebral ischemia using myeloid-specific knockout mice.myeloid-specific mice were subjected to transient (90 minutes) middle artery occlusion followed by 24 hours reperfusion (n=5 7 per group). Ischemic infarcts identified hematoxylin and eosin staining quantified...
Ovarian serous carcinoma is a highly aggressive neoplastic disease in women.Our previous studies have demonstrated Notch3 gene amplification and upregulation many ovarian carcinomas Notch pathway activity contributed to drug resistance.Among different ligands, Jagged1 most dominant cancer, correlated with expression level tissues.In this study, we found that depended on activation.Knockdown of either or RBPjk, Notchinteracting transcription factor critical signaling, suppressed cancer...
Significance How does the heart of a healthy individual cease to function within just few minutes in absence oxygen? We addressed this issue by simultaneously examining and brain animal models during asphyxiation found that asphyxia markedly stimulates neurophysiological neurochemical activities brain. Furthermore, previously unidentified corticocardiac coupling showed increased intensity as deteriorated. Blocking efferent input survival time both The results show targeting brain’s outflow...
Lacunar infarcts and vascular dementia are important phenotypic characteristics of cerebral autosomal dominant arteriopathy with subcortical leukoencephalopathy, the most common inherited small vessel disease. Individuals disease show variability in nature onset symptoms rates progression, which only partially explained by differences pathogenic mutations NOTCH3 gene. Recognizing early its course securing a molecular diagnosis clinical goals, despite lack proven disease-modifying treatments....
We have identified a pineal night-specific ATPase (PINA), novel splice variant of the ATP7B gene disrupted in Wilson disease (WD). PINA expression exhibits dramatic diurnal rhythm both gland and retina with 100-fold greater at night than day. is expressed pinealocytes subset photoreceptors adult rats transiently retinal pigment epithelium ciliary body during development. Nocturnal under control suprachiasmatic nucleus clock mediated by superior cervical ganglion innervation pineal. In vitro...
Background and Purpose— Female, compared with male, animals are protected from cerebral ischemic injury. Physiological concentrations of 17β-estradiol (E2) reduce damage in experimental stroke. E2 augments angiogenesis reproductive organs noncerebral vascular beds. We hypothesized that protects brain stroke through modulation angiogenesis. quantified molecular markers capillary density before after unilateral middle artery occlusion (MCAO). Methods— Female were ovariectomized, treated 25 μg...
Extracellular thrombospondins (TSP or THBS) and the Notch family of transmembrane receptors share a role in multiple, overlapping cellular functions participate developmental signaling pathological reactions to tissue injury. We demonstrate that TSP2, but not TSP1, enhances potency Notch3 signal transduction. In addition, TSP2 reduces cancer cell proliferation Notch-ligand dependent fashion. The loss knock-out mice target gene expression. binds directly Jagged1. TSP1 also Jagged1; however,...
Abstract Indolethylamine N -methyltransferase (INMT) is a transmethylation enzyme that utilizes the methyl donor S -adenosyl-L-methionine to transfer groups amino of small molecule acceptor compounds. INMT best known for its role in biosynthesis N,N -Dimethyltryptamine (DMT), psychedelic compound found mammalian brain and other tissues. In mammals, DMT thought occur via double methylation tryptamine, where first catalyzes -methyltryptamine (NMT) then DMT. However, it unknown whether...
Abstract: Serotonin (5‐hydroxytryptamine, 5‐HT), a precursor for melatonin production, is produced abundantly in the pineal gland of all vertebrate animals. The synthesis 5‐HT rate limited by tryptophan hydroxylase 1 (TPH1) whose activity displays twofold increase at night. Earlier studies from our laboratory demonstrate that secretion exhibits dynamic circadian rhythms with elevated levels during early night, and controlled adrenergic signaling In this study, we report (a) total output TPH1...
Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is the best understood cause of dominantly inherited stroke results from NOTCH3 mutations that lead to protein accumulation selective arterial smooth muscle degeneration. Previous studies show forms multimers. Here, we investigate interactions between other vascular Notch isoforms characterize effects elevated on gene regulation. We demonstrate heterodimers NOTCH1, NOTCH3, NOTCH4. R90C C49Y...