A5056086091- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Chromatography
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Mass Spectrometry Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Electrochemical Analysis and Applications
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Metabolism, Diabetes, and Cancer
- Electrochemical sensors and biosensors
- Electrowetting and Microfluidic Technologies
- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Diabetes and associated disorders
- Neuroscience and Neural Engineering
- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Advanced Chemical Sensor Technologies
- Ion channel regulation and function
University of Michigan
2016-2025
Michigan United
2009-2024
Clearfield (United States)
2024
Royal Society of Chemistry
2023
University of Notre Dame
1997-2023
Michigan State University
2023
The University of Texas at El Paso
2023
Stockholm University
1978-2023
Curtin University
2023
Rutherford Appleton Laboratory
2023
Secretion of catecholamines from single bovine chromaffin cells in culture was elicited by brief pressure ejections a micropipette containing nicotine, carbamoylcholine, or potassium ions mechanical stimulation. Release monitored electrochemically with carbon-fiber microelectrode placed adjacent to the cell. Cyclic voltammetry used identify secreted species, whereas constant potential amperometry for improved temporal resolution (millisecond range) catecholamine detection. During secretion,...
A novel Zn2+-selective visible wavelength fluoroionophore (FluoZin-3, 9) was synthesized. The chelating portion of the molecule resembles known EGTA-based Ca2+-selective fluoroionophores, except that one N-acetic acid moieties has been deleted in 9. FluoZin-3 is virtually non-fluorescent absence Zn2+, and exhibits a several hundred-fold fluorescence increase upon saturation with Zn2+(∼100 nM), Kd = 15 ± 2 nM. 1:1 binding stoichiometry 9:Zn2+ determined, complex pH-independent at pH > 6. used...
The role of the plasma lecithin :cholesterol acyltransferase reaction in esterification cholesterol human and baboon high density lipoproteins has been studied.Human was incubated vitro, initial rate lipoprotein fractions obtained by chromatography on hydroxylapatite determined.The greater fraction than low fraction.High from were filtered through columns Sephadex G 200, relative concentrations effluent key lipids involved ratio esterified to unesterified varied across peak either type...
In vivo neurochemical monitoring using microdialysis sampling is important in neuroscience because it allows correlation of neurotransmission with behavior, disease state, and drug concentrations the intact brain. A significant limitation current practice that different assays are utilized for measuring each class neurotransmitter. We present a high performance liquid chromatography (HPLC)–tandem mass spectrometry method utilizes benzoyl chloride determination most common low molecular...
Abstract The implementation of continuous flow technology is critical towards enhancing the application photochemical reactions for industrial process development. However, there are significant time and resource constraints associated with translating discovery scale vial-based batch to scale-up conditions. Herein we report development a droplet microfluidic platform, which enables high-throughput reaction in generate pharmaceutically relevant compound libraries. This platform allows...
This perspective outlines the major challenges and future prospects for field of droplet microfluidics high throughput screening applications.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and evaluation of packed capillary liquid chromatography columns with inner diameters from 20 to 50 micrometersRobert T. Kennedy James W. JorgensonCite this: Anal. Chem. 1989, 61, 10, 1128–1135Publication Date (Print):May 15, 1989Publication History Published online1 May 2002Published inissue 15 1989https://pubs.acs.org/doi/10.1021/ac00185a016https://doi.org/10.1021/ac00185a016research-articleACS PublicationsRequest reuse...
A DNA aptamer against IgE was labeled with fluorophore and used as a selective fluorescent tag for determining by capillary electrophoresis laser-induced fluorescence detection (CE-LIF). CE-LIF separations of samples containing fluorescently were complete in less than 60 s revealed two zones, one corresponding to free the other bound IgE. The peak decreased increased proportion amount sample so that could be detected linear dynamic range 105 limit 46 pM. assay highly unaffected presence IgG...
We have engineered aptamers that contain fluorescent reporters and signal the presence of cognate ligands in solution. Two different anti-adenosine "signaling aptamers", one made from RNA DNA, can selectively adenosine Increases fluorescence intensity reproducibly follow increases concentration, be used for quantitation. The facile methods we developed potentially generating a wide variety signaling use sensor arrays.
Obesity is characterized by hyperinsulinemia, hyperleptinemia, and an increase in islet volume. While the mechanisms that hasten onset of diabetes obese individuals are not known, it possible adipose-derived hormone leptin plays a role. In addition to its central actions, exerts biological effects acting peripheral tissues including endocrine pancreas. To explore impact disrupting signaling pancreas on β cell growth and/or function, we created pancreas-specific receptor (ObR) KOs using mice...
A microfluidic device has been developed for the determination of insulin secreted from islets Langerhans by a capillary electrophoresis competitive immunoassay. Online assays were performed electrophoretically sampling anti-insulin antibody (Ab), fluorescein isothiocyanate-labeled (FITC-insulin), and separate reservoirs allowing them to mix as they traveled through 4-cm reaction channel heated 38 °C. From channel, samples injected onto 1.5-cm-long where FITC-insulin FITC-insulin−Ab complex...
Functional insulin receptors are known to occur in pancreatic beta cells; however, except for a positive feedback on synthesis, their physiological effects unknown. Amperometric measurements at single, primary cells reveal that application of exogenous the presence or absence nonstimulatory concentrations glucose evokes exocytosis mediated by cell receptor. Insulin also elicits increases intracellular Ca2+ concentration but has minimal membrane potential. Conditions where receptor is blocked...
A simple, fast, and reproducible sample preparation procedure was developed for relative quantification of metabolites in adherent mammalian cells using the clonal β-cell line INS-1 as a model sample. The method by evaluating effect different procedures on high performance liquid chromatography- mass spectrometry 27 involved glycolysis tricarboxylic acid cycle directed basis well all detectable chromatographic features an undirected basis. We demonstrate that rapid water rinse step prior to...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRapid immunoassays using capillary electrophoresis with fluorescence detectionNicole M. Schultz and Robert T. KennedyCite this: Anal. Chem. 1993, 65, 21, 3161–3165Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/ac00069a035https://doi.org/10.1021/ac00069a035research-articleACS PublicationsRequest reuse permissionsArticle...
2-Bromohexadecanoic acid, or 2-bromopalmitate, was introduced nearly 50 years ago as a nonselective inhibitor of lipid metabolism. More recently, 2-bromopalmitate re-emerged general protein S-palmitoylation. Here, we investigate the cellular targets through synthesis and application click-enabled analogues. In cells, is converted to 2-bromopalmitoyl-CoA, although less efficiently than free palmitate. Once conjugated CoA, probe reactivity dramatically enhanced. Importantly, both...
Quantification of insulin release from pancreatic islets Langerhans is interest for diabetes research. Typical secretion experiments are performed using offline techniques that expensive, slow, have low-throughput, and require multiple islets. We developed a microfluidic device high-throughput, automated, online monitoring individual in parallel. This chip consists 15 channel networks each capable superfusing single islet mixing superfusate with fluorescein isothiocyanate-labeled...