A5042665987- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- CRISPR and Genetic Engineering
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Invertebrate Immune Response Mechanisms
- Identification and Quantification in Food
- Immune Response and Inflammation
- Marine Invertebrate Physiology and Ecology
- Viral Infectious Diseases and Gene Expression in Insects
- Fish Ecology and Management Studies
- Social and Educational Sciences
- Aquaculture Nutrition and Growth
- MicroRNA in disease regulation
- Education, Healthcare and Sociology Research
- Environmental DNA in Biodiversity Studies
- Phytochemicals and Antioxidant Activities
- Hops Chemistry and Applications
- Antimicrobial Peptides and Activities
- Nutrition, Genetics, and Disease
- Planarian Biology and Electrostimulation
- Bacterial biofilms and quorum sensing
- Environmental Toxicology and Ecotoxicology
Nofima
2017-2024
Fafo Foundation
2017-2023
Norwegian University of Life Sciences
2014-2017
University of Oslo
2017
UiT The Arctic University of Norway
2006-2016
The Norwegian aquaculture of Atlantic salmon (Salmo salar L.) is hampered by ulcerative disorders associated with bacterial infections. Chronic ulceration may provide microenvironments that disturb the normal microbial biodiversity external surfaces. Studying composition communities in skin ulcers will enhance our understanding ulcer aetiology. To achieve this, we tested marine farmed and sampled base edge at end winter (April) summer (September), addition to mucus healthy individuals. In...
There is an increasing interest in the impact of feed on fish gut microbiome. Most studies are based sequencing bacterial housekeeping gene 16S rRNA from extracted total DNA, including resident and non-resident live bacteria as well dead bacteria. It has not been a common practice to include control, although it contains various nutritious ingredients that microorganisms can use before or after preparation. Thus, study designs using digesta proxy for intestinal microbiome raise concern...
Atlantic salmon farming operates with high production intensities where skin integrity is recognized as a central factor and indicator for animal health welfare. In the described trial, development its immune status in healthy reared two different systems, traditional open net-pen system semi-closed containment system, were investigated. Freshwater smolts compared to post-smolts after 1 4 months seawater. Growth performance, when adjusted temperature, was equal between systems. Skin...
Aliivibrio wodanis and Moritella viscosa have often been isolated concurrently from fish with winter-ulcer disease. Little is known about the interaction between two bacterial species how presence of one affects behaviour other. The impact on growth in co-culture was investigated vitro, A. has an inhibitorial effect M. viscosa. Further, we sequenced complete genomes these marine Gram-negative species, performed transcriptome analysis gene expression levels vivo samples. Using implants...
The aim of this study was to use a sensitive method screen and quantify 57 Vibrionaceae strains for the production acyl-homoserine lactones (AHLs) map resulting AHL profiles onto host phylogeny.We used high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) protocol measure AHLs in spent media after bacterial growth. First, presence/absence (qualitative analysis) measured choose internal standard subsequent quantitative measurements. We screened from three genera...
Abstract Understanding fish‐microbial relationships may be of great value for fish producers as growth, development and welfare are influenced by the microbial community associated with rearing systems surfaces. Accurate methods to generate analyze these communities would an important tool help improve understanding effects in industry. In this study, we performed taxonomic classification determination operational units on Atlantic salmon microbiota taking advantage full‐length 16S rRNA gene...
Abstract The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, exhibits high salt tolerance over wide range temperatures has extra-cellular hydrolytic activities several substrates, indicating it secretes may function salinity conditions. Genome sequencing identified the genes involved...
Transcriptomics provides valuable data for functional annotations of genes, the discovery biomarkers, and quantitative assessment responses to challenges. Meta-analysis Nofima’s Atlantic salmon microarray database was performed selection genes that have shown strong reproducible expression changes. Using from 127 experiments including 6440 microarrays, four transcription modules (TM) were identified with a total 902 annotated genes: 161 virus responsive – VRG (activated five viruses poly...
There is an increased interest in identifying beneficial compounds of plant origin that can be added to animal diets improve performance and have a health-promoting effect. In the present study, nine herb species Norwegian wild flora or which cultivated Norway were selected for phytogenic evaluation (hops, maral root, mint, oregano, purslane, rosemary, roseroot, sweet wormwood, yarrow). Dried herbs sequentially extracted with dichloromethane (DCM), ethanol (EtOH) finally water (H2O) by...
Skin is the first line of defence against waterborne pathogens, such as Moritella viscosa, which causative agent winter ulcer disease in Atlantic salmon (Salmo salar L.). The present study revealed that vaccination protects both M. viscosa-induced mortality and skin ulceration, but protection varied according to strain used vaccine formulation. Examination tissue 4 days post viscosa challenge indicated initiated infection by colonising scale surface. Sequencing transcripts variable region...
Genus Aliivibrio is known to harbor species exhibiting bioluminescence as well pathogenic behavior affecting the fish farming industry. Current phylogenetic understanding of has largely remained dormant after reclassification disentangled it from Vibrio genus in 2007. There growing evidence wider diversity, but until now lack genomes and selective use type strains have limited ability compare classify firmly. In this study, a total 143 bacterial strains, including 51 novel sequenced were...
Focal dark spots (DS) in farmed Atlantic salmon fillets contain a significant number of B cells as revealed by the high abundance immunoglobulin (Ig) transcripts transcriptome data. The immune response DS remains unknown while they represent major problem commercial aquaculture. Here, we characterized diversity and clonal composition DS. Sixteen gene markers antigen presentation were analyzed with RT-qPCR. All genes expression showed positive correlation area intensity. flatter DS, higher...
Moritella viscosa is the aetiological agent of winter-ulcer disease in farmed salmonids North Atlantic. Previously, two major (typical and variant) genetic clades have been demonstrated within this bacterial species, one which almost solely related to Atlantic salmon (Salmo salar). In present study infection trials that 'typical' M. isolated from Norwegian was highly virulent fish species but resulted lower levels mortality rainbow trout. 'Variant' trout modest both To investigate possible...
Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. comprises two main clusters that differ genetic variation and phenotypes including virulence. Horizontal gene transfer through acquisition loss of mobile elements (MGEs) is major driving force bacterial diversification. To gain insight into genomic traits could affect sublineage evolution within this bacterium we examined the genome sequences twelve strains. Matches...
The salmon louse (Lepeophtheirus salmonis) is a significant problem in Atlantic aquaculture. industry forced to use harsh antiparasitic treatments, including hydrogen peroxide (H2O2) baths, treat the fish. We have utilized vivo, semi and vitro methods study effects of exposures H2O2. Assessment external welfare indicators plasma levels cortisol, glucose, lactate, ions did not reveal systemic impacts. Transcriptome analyses showed acute changes skin, with transient activation several...
Recirculating aquaculture systems (RAS) are receiving greater attention because they have potential to reduce the use of water, enable better control environmental conditions and give biosecurity. However, little is known about effects in RAS on early development. Here we compare survival, development gene expression Atlantic salmon grown a flow through (FT) system from egg until before start feed (at around 875°d). The epidermis fish was significantly thinner contained larger numbers...