A5006898852- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Metabolism, Diabetes, and Cancer
- Mass Spectrometry Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Lanthanide and Transition Metal Complexes
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Protein purification and stability
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Computational Drug Discovery Methods
University of Graz
2022-2024
Universität Innsbruck
2020-2023
ING Direct
2023
The relative orientation of the two variable domains, VH and VL , influences shape antigen binding site, that is, paratope, is essential to understand specificity. ABangle characterizes -VL by using five angles a distance compares it other known structures. Molecular dynamics simulations antibody domains (Fvs) reveal fluctuations in domain orientations. observed between these are confirmed NMR experiments on single-chain fragment (scFv) complex with IL-1β an antigen-binding (Fab)....
The analysis of gangliosides is extremely challenging, given their structural complexity, lack reference standards, databases, and software solutions. Here, we introduce a fast 6 min high field asymmetric ion mobility spectrometry (FAIMS) shotgun lipidomics workflow, along with dedicated solution for ganglioside detection. By ramping FAIMS compensation voltages, ideal ranges different classes were obtained. revealed both class- charge-state separation behavior based on the glycan headgroup...
Gangliosides are an indispensable glycolipid class concentrated on cell surfaces with a critical role in stem differentiation. Nonetheless, owing to the lack of suitable methods for scalable analysis covering full scope ganglioside molecular diversity, their mechanistic properties signaling and differentiation remain undiscovered large extent. This work introduces sensitive comprehensive assay based liquid chromatography, high-resolution mass spectrometry, multistage fragmentation....
The analysis of the glycosphingolipid subclass gangliosides is extremely challenging, given their structural complexity, lack reference standards, databases, and software solutions. Here, we introduce a fast 6 min High Field Asymmetric Ion Mobility Spectrometry (FAIMS) shotgun-based lipidomics workflow for improved ganglioside detection. By ramping compensation voltages, ideal ranges different classes were obtained. FAIMS revealed both class- charge-state separation behavior based on glycan...
Abstract In the last years, antibodies have emerged as a promising new class of therapeutics, due to their combination high specificity with long serum half‐life and low risk side‐effects. Diabodies are popular novel antibody format, consisting two F v domains connected short linkers. Like IgG antibodies, they simultaneously bind target proteins. However, offer altered properties, given smaller size higher rigidity. this study, we conducted the—to our knowledge—first molecular dynamics (MD)...
Abstract β‐glucosidases play a pivotal role in second‐generation biofuel (2G‐biofuel) production. For this application, thermostable enzymes are essential due to the denaturing conditions on bioreactors. Random amino acid substitutions have originated new β‐glucosidases, but without clear understanding of their molecular mechanisms. Here, we probe by different dynamics simulation approaches with distinct force fields and submitting results various computational analyses, bases...
Abstract Gangliosides are an indispensable glycolipid class concentrated on cell surfaces with a critical role in stem differentiation. Nonetheless, owing to the lack of suitable methods for scalable analysis covering full scope ganglioside molecular diversity, their mechanistic properties signaling and differentiation remain undiscovered large extent. This work introduces sensitive comprehensive assay based liquid chromatography, high-resolution mass spectrometry, multistage fragmentation....
Pinpointing double bond (C=C) positions in native lipid extracts is beyond the capabilities of standard mass spectrometry-based approaches. This article highlights a novel untargeted workflow supported by open-source software MS-RIDD, that allows for semi-automated annotation C=C locations with high confidence.