A5063992837- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Insect and Pesticide Research
- Venomous Animal Envenomation and Studies
- Pharmacological Receptor Mechanisms and Effects
- Neurobiology and Insect Physiology Research
- Computational Drug Discovery Methods
- EEG and Brain-Computer Interfaces
- Neuroendocrine regulation and behavior
- Synthesis and Biological Evaluation
- Retinal Development and Disorders
- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Advanced Sensor and Energy Harvesting Materials
- Cardiac electrophysiology and arrhythmias
- Neurotransmitter Receptor Influence on Behavior
- Attention Deficit Hyperactivity Disorder
- Advanced Memory and Neural Computing
- Chemical synthesis and alkaloids
- Neuropeptides and Animal Physiology
HiQScreen (Switzerland)
2015-2024
KU Leuven
2022
Genome Institute of Singapore
2017
AbbVie (United States)
2015
Georgia Regents Medical Center
2014
Augusta University
2014
Roche (Switzerland)
2014
Psychogenics (United States)
2014
University of Geneva
2002-2013
Hôpital Necker-Enfants Malades
2011
Intracellular current administration evokes rapid, graded, and bidirectional mechanical responses of isolated outer hair cells from the mammalian inner ear. The become shorter in response to depolarizing longer hyperpolarizing currents synaptic end cell. respond with either an increase or decrease length transcellular alternating stimulation. direction movement stimuli appears be frequency dependent. Iontophoretic application acetylcholine cell decreases its length. microarchitecture organ...
Several lines of evidence suggest a link between the alpha7 neuronal nicotinic acetylcholine receptor (nAChR) and brain disorders including schizophrenia, Alzheimer's disease, traumatic injury. The present work describes novel molecule, 1-(5-chloro-2,4-dimethoxy-phenyl)-3-(5-methyl-isoxazol-3-yl)-urea (PNU-120596), which acts as powerful positive allosteric modulator nAChR. Discovered in high-throughput screen, PNU-120596 increased agonist-evoked calcium flux mediated by an engineered...
The relative permeability for sodium, potassium, and calcium of chicken alpha 7 neuronal nicotinic receptor was investigated by mutagenesis the channel domain M2. Mutations in "intermediate ring" negatively charged residues, located at cytoplasmic end M2 (site 1), reduce without significantly modifying other functional properties (activation desensitization) receptor; a similar change ion selectivity is also noticed when mutations site 1 are done context mutant that conducts ions...
1. Solitary rod inner segments were obtained by enzymatic dissociation of the tiger salamander ( Ambystoma tigrinum ) retina. Their membrane currents studied with single‐pipette voltage‐clamp technique. Individual isolated aid pharmacological agents. 2. Extracellular caesium blocked a current activated hyperpolarization from ‐30 mV. Changing external sodium and potassium concentrations altered value reversal potential in manner consistent being carried equally both ions. 3....
Autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) is the first, and to date only, idiopathic for which a specific mutation has been found. A missense in critical M2 domain of a4 subunit neuronal nicotinic acetylcholine receptor (CHRNA4) recently identified one large Australian pedigree. Here we describe novel CHRNA4 gene Norwegian family. Three nucleotides (GCT) were inserted at nucleotide position 776 into coding region C-terminal end domain. Physiological investigations...
Widely expressed in the brain, alpha4beta2 nicotinic acetylcholine receptor (nAChR) is proposed to play a major role mechanisms that lead and maintain nicotine addiction. Using patch-clamp technique pharmacological protocols, we examined consequences of long-term exposure 0.1-10 micrometer K-177 cells expressing human brain receptor. The dose-response curves are biphasic revealed both high- low-affinity component with apparent EC(50) values 1.6 62 micrometer. Ratios receptors components 25...
We report that preapplication of ivermectin, in the micromolar range, strongly enhances subsequent acetylcholine-evoked current neuronal chick or human α7 nicotinic acetylcholine receptors reconstituted <i>Xenopus laevis</i> oocytes and K-28 cells. This potentiation does not result from nonspecific Cl<sup>−</sup>currents. The concomitant increase apparent affinity cooperativity dose-response curve suggest ivermectin acts as a positive allosteric effector. interpretation is supported by...
The major brain nicotinic acetylcholine receptor is assembled from two subunits termed alpha 4 and n 1. When expressed in Xenopus oocytes, these reconstitute a functional that inhibited by progesterone levels similar to those found serum. In this report, we show the steroid interacts with site located on extracellular part of protein, thus confirming inhibition not due nonspecific perturbation membrane bilayer or activation second messengers. Because does require presence agonist,...
The putative channel-forming MII domains of the nicotinic, gamma-aminobutyric acid type A, and glycine receptors contain a highly conserved leucine residue. Mutation this hydrophobic amino in neuronal nicotinic receptor alpha 7 (Leu-247), reconstituted Xenopus oocytes, modifies ionic response to acetylcholine alters desensitization. Furthermore, Leu----Thr (L247T) mutant has two conducting states (46 pS 80 pS), contrast with wild-type (WT) receptor, which only one (45 pS). We now show that...
To present a novel and minimally invasive approach to intraocular pressure (IOP) monitoring based on sensing contact lens.The key element of this measurement method is soft lens with an embedded microfabricated strain gauge allowing the changes in corneal curvature correlated variations IOP. A prototype was adapted tested enucleated porcine eyes. verify principle device, posterior chamber pig eyes cannulated connected syringe pump sensor for precise control The measurements were then...
1. The recently isolated compound methyllycaconitine (MLA) is a plant toxin which competitive inhibitor of nicotinic acetylcholine receptors (nAChRs). We found that homomeric alpha 7 display very high sensitivity to MLA with an IC50 in the picomolar range. 2. nature blockade was reinforced by observation this has no action on wild‐type serotoninergic (5‐HT3), whereas it powerful antagonist chimaeric 7‐5‐HT3. 3. time course inhibition (WT) follows monotonic exponential decay whose constant...
We present herein the cloning of human nicotinic acetylcholine receptor α9-ortholog and identification a new α-like subunit (α10) that shares 58% identity with α9. Whereas α10 fails to produce functional receptors alone, it promoted robust acetylcholine-evoked currents when coinjected The presence modifies physiological pharmacological properties α9 indicating two subunits coassemble in single receptor. Fusing N-terminal domain rest α10-cDNA yielded α9:α10-chimera displays binding ionic pore...