A5082035669- Transportation Planning and Optimization
- Traffic control and management
- Photosynthetic Processes and Mechanisms
- Traffic Prediction and Management Techniques
- Drug Solubulity and Delivery Systems
- Mitochondrial Function and Pathology
- Analytical Chemistry and Chromatography
- Multi-Criteria Decision Making
- Urban Transport and Accessibility
- Plant biochemistry and biosynthesis
- Photoreceptor and optogenetics research
- Advancements in Transdermal Drug Delivery
- Transportation and Mobility Innovations
- Spectroscopy and Chemometric Analyses
- Natural product bioactivities and synthesis
- ATP Synthase and ATPases Research
- Optimal Experimental Design Methods
- Polysaccharides Composition and Applications
- Metalloenzymes and iron-sulfur proteins
- Genomics and Phylogenetic Studies
- BIM and Construction Integration
- Plant-Microbe Interactions and Immunity
- Transportation Safety and Impact Analysis
- Lipid Membrane Structure and Behavior
- Underground infrastructure and sustainability
Norwich Research Park
2023-2024
John Innes Centre
2023-2024
Protein Research Foundation
2004-2018
Osaka University
1999-2018
ShinEtsu Chemical (Japan)
2016-2017
Iwate University
2016
Hoshi University
2009-2012
East Japan Railway (Japan)
2012
Ebara (Japan)
2010
University of Delaware
2002-2005
Chloroplast Translocon Revealed Protein translocation across biological membranes requires supramolecular complexes, called translocons. Chloroplasts require translocons in their double-envelope to import thousands of nucleus-encoded proteins synthesized the cytosol. However, identity translocon at inner envelope chloroplast (TIC) has long been a matter debate; two proteins, Tic20 and Tic110, have proposed be central protein membrane. Using transgenic Arabidopsis plants expressing tagged...
The Chilean soapbark tree ( Quillaja saponaria ) produces soap-like molecules called QS saponins that are important vaccine adjuvants. These highly valuable compounds sourced by extraction from the bark, and their biosynthetic pathway is unknown. Here, we sequenced Q. genome. Through genome mining combinatorial expression in tobacco, identified 16 enzymes together enable production of advanced intermediates represent a bridgehead for adjuvant bioengineering. We further needed to make QS-7,...
Abstract QS-21 is a potent vaccine adjuvant currently sourced by extraction from the Chilean soapbark tree. It key component of human vaccines for shingles, malaria, coronavirus disease 2019 and others under development. The structure consists glycosylated triterpene scaffold coupled to complex 18-carbon acyl chain that critical immunostimulant activity. We previously identified early pathway steps needed make glycoside scaffold; however, biosynthetic route chain, which stimulation T cell...
QS-21 is a potent vaccine adjuvant and remains the only saponin-based that has been clinically approved for use in humans
Chloroplasts import thousands of nucleus-encoded preproteins synthesized in the cytosol through TOC and TIC translocons on outer inner envelope membranes, respectively. Preprotein translocation across membrane requires ATP; however, motor has remained unclear. Here, we report that a 2-MD heteromeric AAA-ATPase complex associates with functions as motor, directly interacting various translocating preproteins. This consists protein encoded by previously enigmatic chloroplast gene ycf2 five...
Abstract The biosynthesis of iron-sulfur clusters is a highly regulated process involving several proteins. Among them, so-called scaffold proteins play pivotal roles in both the assembly and delivery clusters. Here, we report identification two chloroplast-localized NifU-like proteins, AtCnfU-V AtCnfU-IVb, from Arabidopsis (Arabidopsis thaliana) with high sequence similarity to cyanobacterial protein that was proposed serve as molecular scaffold. constitutively expressed tissues...
Abstract Chloroplast protein import is mediated by two hetero-oligomeric complexes, the Tic and Toc translocons, which are located in inner outer envelope membranes. At membrane, many components have been identified characterized, but it remains unclear how these proteins organized to form a protein-conducting channel or whether stable core complex that binds translocating preproteins exists. Here, we report identification of 1-megadalton (MD) translocation as an intermediate during across...
The preprotein translocon at the outer envelope membrane of chloroplasts (Toc) mediates recognition and import nuclear-encoded preproteins into chloroplasts. Two receptor components, Toc159 Toc34, channel Toc75 form Toc complex. In this study, we have analyzed molecular architecture organization complex by blue native PAGE (BN-PAGE), which is a high-resolution method for separating protein complexes under non-denaturing conditions. Pea isolated in presence protease inhibitor cocktail were...
Abstract Soapwort ( Saponaria officinalis ) is a flowering plant from the Caryophyllaceae family with long history of human use as traditional source soap. Its detergent properties are because production polar compounds (saponins), which oleanane-based triterpenoid saponins, saponariosides A and B, major components. saponins have anticancer also interest endosomal escape enhancers for targeted tumor therapies. Intriguingly, these share common structural features vaccine adjuvant QS-21 and,...
Chloroplasts change their position to adapt cellular activities fluctuating environmental light conditions. Phototropins (phot1 and phot2 in Arabidopsis) are plant-specific blue photoreceptors that perceive changes intensity direction, mediate actin-based chloroplast photorelocation movements. Both phot1 regulate the accumulation response, while is mostly responsible for regulation of avoidance response. Although it has been widely accepted distinct intracellular localizations phototropins...
Menthosomes, novel deformable carriers for the enhancement of transdermal delivery are introduced in this study. Meloxicam (MX)-loaded menthosomes were formulated, and their physicochemical characteristics skin permeability evaluated. A two-factor spherical second-order composite experimental design was used to prepare formulation menthosomes. Ten formulations composed a phospholipid as lipid bilayer carrier, cholesterol (Chol) stabilizer cetylpyridinium chloride (CPC) L-menthol penetration...
Chloroplasts are responsible for biosynthesis of salicylic acid (SA) an important signal molecule in plant immunity. EDS5 is a homolog the MATE (multidrug and toxic compound extrusion) family transporters, essential SA biosynthesis. It has been speculated that would be involved export from chloroplasts. However, subcellular localization remains largely uncharacterized. We demonstrate here specifically localized to chloroplast envelope membrane Arabidopsis. In addition, we found...
We isolated an Arabidopsis thaliana cDNA whose translated product shows sequence similarity to the FtsY, a bacterial homologue of SRP receptor protein. The FtsY contains typical chloroplast transit peptide. in vitro‐synthesized 37 kDa was imported into chloroplasts, and processed 32 polypeptide bound peripherally on outer surface thylakoids. Antibodies raised against also reacted with thylakoid‐bound antibodies inhibited cpSRP‐dependent insertion light‐harvesting chlorophyll / b ‐binding...
Abstract A chloroplast signal recognition particle (SRP) that is related to the SRP involved in secretion bacteria and eukaryotic cells used for insertion of light-harvesting chlorophyll proteins (LHCPs) into thylakoid membranes. conserved component mechanism a membrane-bound receptor, denoted FtsY bacteria. Plant genomes encode homologs are targeted (cpFtsY). To investigate vivo roles cpFtsY, we characterized maize cpFtsY mutants having Mu transposon corresponding gene (chloroplast...
Fuzzy set theory is applied to solve the problem of traffic assignment between two alternative routes on a highway network. The driver's perceived travel time each route treated as fuzzy number, and his choice based an approximate reasoning model. model consists rules which indicate degree preference for given routes. Applying driver then aggregating individual preferences, network loading algorithm assigns route.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML"...
The insertion of light-harvesting chlorophyll proteins (LHCPs) into the thylakoid membrane chloroplast is cpSRP-dependent, and requires stromal components cpSRP54 cpSRP43, membrane-bound SRP receptor cpFtsY integral protein Alb3. Previous studies demonstrated that Arabidopsis mutant lacking both cpSRP43 had pale yellow leaves, but was viable, whereas mutants Alb3 exhibit an albino phenotype more severe seedling lethality. We previously showed a maize yellow-green lethal. To compare in vivo...
Protein translocation across the inner envelope of plastids is mediated by TIC (translocon at membrane chloroplasts) protein machinery. Tic20 has been shown to function as a central component The Arabidopsis genome encodes four homologous proteins, AtTic20-I, AtTic20-II, AtTIC20-IV and AtTic20-V, among which only AtTic20-I extensively characterized demonstrated be essential for import into chloroplasts. more closely related AtTic20-IV than AtTic20-II or whereas AtTic20-V show higher...
ABSTRACT The aims of this study were to assess the processing window hypromellose acetate succinate (HPMCAS) as a polymeric carrier for hot‐melt extrusion, and investigate effect screw configuration parameters on physicochemical properties HPMCAS extrudates. processability was investigated using Brabender® mixer Pharma 11 Twin Screw Extruder. extrudates characterized with regard glass transition temperature, true density, yellowness index, crystallinity, morphology, free succinic acid/acetic...
The furan ring is a defining feature of limonoids, class highly rearranged and bioactive plant tetranortriterpenoids. We recently reported an apparent complete biosynthetic pathway to these important natural furanoids. Herein, we disclose the subsequent discovery yield-boosting "missing link" carboxylesterase that selectively deprotects late-stage intermediate, so triggering more efficient biosynthesis. This has allowed, for first time, isolation structural elucidation unknown intermediates,...
Railway transportation is one of the most eco-friendly way. The amount energy consumption, however, for railway not so small that it important to make effort enhance eco-friendliness continuously realize low carbon society in future. In this paper, tested and calculated impact high efficiency power supply saving technologies system traction station, office etc. are compared. As an advanced technology, only storage devices regenerative utilization but also smart grid technology mentioned....