A5023569800- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Glycosylation and Glycoproteins Research
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Pharmacological Effects of Natural Compounds
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- Carbohydrate Chemistry and Synthesis
QB3
2024
University of California, Berkeley
2024
Joint BioEnergy Institute
2023-2024
Northwestern University
2017-2020
QS-21 is a potent vaccine adjuvant and remains the only saponin-based that has been clinically approved for use in humans
Glycosylation of biomolecules can greatly alter their physicochemical properties, cellular recognition, subcellular localization, and immunogenicity. reactions rely on the stepwise addition sugars using nucleotide diphosphate (NDP)-sugars. Making these substrates readily available will accelerate characterization new glycosylation reactions, elucidation underlying regulation mechanisms, production glycosylated molecules. In this work, we engineered Saccharomyces cerevisiae to heterologously...
Methyl jasmonate (MeJA) is a known elicitor of plant specialized metabolism, including triterpenoid saponins. Saponaria vaccaria an annual herb used in traditional Chinese medicine, containing large quantities oleanane-type saponins with anticancer properties and structural similarities to the vaccine adjuvant QS-21. Leveraging MeJA-elicited saponin biosynthesis, we identify multiple enzymes catalyzing oxidation glycosylation triterpenoids S. vaccaria. This exploration aided by Pacbio...
Glycosylation is a ubiquitous modification present across all of biology, affecting many things such as physicochemical properties, cellular recognition, subcellular localization, and immunogenicity. Nucleotide sugars are important precursors needed to study glycosylation produce glycosylated products. Saccharomyces cerevisiae potentially powerful platform for producing biomolecules, but it lacks nucleotide sugar diversity. metabolism complex, understanding how engineer will be necessary...
Abstract Microbial cell factories offer an attractive approach for production of biobased products. Unfortunately, designing, building, and optimizing biosynthetic pathways remains a complex challenge, especially industrially-relevant, non-model organisms. To address this we describe platform in vitro P rototyping R apid O ptimization B iosynthetic E nzymes (iPROBE). In iPROBE, lysates are enriched with enzymes by cell-free protein synthesis then metabolic assembled mix-and-match fashion to...