A5103175263- Marine and coastal ecosystems
- Toxic Organic Pollutants Impact
- Carcinogens and Genotoxicity Assessment
- Library Science and Administration
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- DNA Repair Mechanisms
- Berry genetics and cultivation research
- Plant tissue culture and regeneration
- Salmonella and Campylobacter epidemiology
- Analytical chemistry methods development
- Algal biology and biofuel production
- Horticultural and Viticultural Research
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Library Science and Information Literacy
- Microbial bioremediation and biosurfactants
- Geochemistry and Elemental Analysis
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
Donald Danforth Plant Science Center
2012-2020
Ionis Pharmaceuticals (United States)
2016
Oregon State University
2009-2011
McMaster University
1979-2000
York University
1978
Harvard University Press
1961
Alternative splicing can enhance transcriptome plasticity and proteome diversity. In plants, alternative be manifested at different developmental stages, is frequently associated with specific tissue types or environmental conditions such as abiotic stress. We mapped the Arabidopsis single-base resolution using Illumina platform for ultrahigh-throughput RNA sequencing (RNA-seq). Deep confirmed a majority of annotated introns identified thousands novel alternatively spliced mRNA isoforms. Our...
Phenotyping has become the rate-limiting step in using large-scale genomic data to understand and improve agricultural crops. Here, Bellwether Platform for controlled-environment plant growth automated multimodal phenotyping is described. The system capacity 1140 plants, which pass daily through stations record fluorescence, near-infrared, visible images. Plant Computer Vision (PlantCV) was developed as open-source, hardware platform-independent software quantitative image analysis. In a...
Abstract Teff ( Eragrostis tef ) is a cornerstone of food security in the Horn Africa, where it prized for stress resilience, grain nutrition, and market value. Here, we report chromosome-scale assembly allotetraploid teff (variety Dabbi) patterns subgenome dynamics. The genome contains two complete sets homoeologous chromosomes, with most genes maintaining as syntenic gene pairs. TE analysis allows us to estimate that polyploidy event occurred ~1.1 million years ago (mya) subgenomes...
Analysis of partially purified crude extract Escherichia coli K12 by chromatography and gel electrophoresis has resulted in the separation three distinct activities which catalyse reduction nitrofurazone (semicarbazone 5-nitro-2-furaldehyde) presence oxygen (type I nitroreductases). The major enzymatic activity IA), was dependent solely on NADPH as a cofactor, absent from nitrofurazone-resistant strains NFR 402 502, but present SIL 41, strain is only marginally resistant to nitrofuran....
Spirodela polyrhiza is a fast-growing aquatic monocot with highly reduced morphology, genome size and number of protein-coding genes. Considering these biological features its basal position in the lineage, understanding architecture could shed light on plant adaptation evolution. Like many draft genomes, however, 158-Mb sequence has not been resolved to chromosomes, important characteristics have defined. Here we deployed rapid genome-wide physical maps combined high-coverage short-read...
Rubus (Rosaceae) comprises more than 500 species with additional commercially cultivated raspberries and blackberries. The most recent (> 100 years old) global taxonomic treatment of the genus defined 12 subgenera; two subgenera were subsequently described some rearranged. Intra- interspecific ploidy levels hybridization make phylogenetic estimation challenging. Our objectives to estimate phylogeny 94 taxonomically geographically diverse three cultivars using chloroplast DNA sequences target...
Background Switchgrass (Panicum virgatum L.) is a C4 perennial grass and widely popular as an important bioenergy crop. To accelerate the pace of developing high yielding switchgrass cultivars adapted to diverse environmental niches, generation genomic resources for this plant necessary. The large genome size polyploid nature makes whole sequencing daunting task even with current technologies. Exploring transcriptional landscape using next technologies provides viable alternative in...
Abstract Motivation: High-throughput sequencing technologies have recently made deep interrogation of expressed transcript sequences practical, both economically and temporally. Identification intron/exon boundaries is an essential part genome annotation, yet remains a challenge. Here, we present supersplat, method for unbiased splice-junction discovery through empirical RNA-seq data. Results: Using genomic reference high-throughput datasets, supersplat empirically identifies potential...
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed locus for aphid resistance. Black (Rubus occidentalis L.) is high-value crop in the Pacific Northwest North America with an international marketplace. Few resources are readily available little improvement has been achieved through breeding efforts to address production challenges involved growing this crop. Contributing its lack low diversity elite cultivars untapped reservoir...
Abstract The extraction of polycyclic aromatic hydrocarbons (PAH) from sediments and an urban dust standard reference material (SRM 1649) was compared using two methods; ultrasonication Soxhlet extraction. Sample weights ranging 0.5 g to 5 were extracted ultrasonication. yield organic sediment samples with solvents 2.53 ± 0.10% while the method yielded 2.41 0.14% initial sample weight. Sequential ultrasonic much more rapid (45 minutes) than (two days) resulted in equal efficiency. levels PAH...
Centralization and Decentralization In Academic Libraries: A Symposium - Introduction; at Harvard; Cornell; Boston
We have determined the mutational specificity of 5-nitrofuran derivative furylfuramide (AF2) in lacI gene Escherichia coli. Treatment a ΔuvrB, pKM101 strain with 1 M AF2 yielded mutation frequency ˜300 times greater than that untreated controls. Of 165 AF2-induced mutants analysed by DNA sequencing, 145 were base substitution mutations, 11 frameshifts, and remainder small deletions, tandem substitutions complex mutations. Base occurred primarily (> 93%) at G:C pairs. The proportions various...
A collaborative study was conducted to compare a new enrichment procedure for the TECRA Salmonella Visual Immunoassay (TSVIA) with reference method given in U.S. Food and Drug Administration's Bacteriological Analytical Manual (7th Ed.). Three food types (milk powder, pepper, soy flour) were analyzed Australia 3 chocolate, dried egg, raw turkey) United States. Thirty-eight collaborators participated study. The evaluated using both Rappaport-Vassiliadis R10 (RV(R10)) tetrathionate (TT) broths...
Five foods types were analyzed by the Assurance EHEC (Escherichia coli O157:H7) enzyme immunoassay (EIA) and Bacteriological Analytical Manual (BAM) culture method. Each sample of each food type at inoculation level was simultaneously both methods. A total 21 laboratories representing state federal government agencies private industry in United States Canada participated. Samples inoculated with E. O157:H7, except for one lot poultry that naturally contaminated. 1304 samples controls...