A5074386455- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Molecular Biology Techniques and Applications
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- RNA and protein synthesis mechanisms
- Plant Pathogenic Bacteria Studies
- Nuclear Receptors and Signaling
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Biofuel production and bioconversion
- Soybean genetics and cultivation
- Folate and B Vitamins Research
- Plant pathogens and resistance mechanisms
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Bioenergy crop production and management
- Neurological diseases and metabolism
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- GABA and Rice Research
Jianghan University
2013-2024
Chongqing University
2024
Fuling Center Hospital of Chongqing
2024
Gansu Academy of Agricultural Sciences
2023
Chifeng Municipal Hospital
2017-2022
China National Hybrid Rice R&D Central Hunan Hybrid Rice Reserch Center
2021
State Key Laboratory of Hybrid Rice
2021
Beijing Technology and Business University
2021
Ministry of Agriculture
2016
Sichuan Tourism University
2016
The plant genome can respond rapidly and dynamically to stress in a manner that overcomes the restrictions of highly stable DNA sequence. Abiotic stresses such as chilling, planting density, rubbing, cutting, successive rounds subculture generally decrease levels methylation. opposite effect is seen for salt stress, effects heavy-metal are species specific. Biotic pathogenic infection lead two contrasting on methylation plants: hypermethylation genome-wide level hypomethylation...
Spirodela polyrhiza is a fast-growing aquatic monocot with highly reduced morphology, genome size and number of protein-coding genes. Considering these biological features its basal position in the lineage, understanding architecture could shed light on plant adaptation evolution. Like many draft genomes, however, 158-Mb sequence has not been resolved to chromosomes, important characteristics have defined. Here we deployed rapid genome-wide physical maps combined high-coverage short-read...
Abstract APETALA2/ethylene-responsive element binding protein (AP2/EREBP) transcription factors constitute one of the largest and most conserved gene families in plant play essential roles growth, development stress response. Except a few members, AP2/EREBP family has not been characterized Brachypodium distachyon , model Poaceae. We performed genome-wide study this B. by phylogenetic analyses, transactivation assays transcript profiling. A total 149 genes were identified divided into four...
Salt stress responses of 23 asparagus bean cultivars were evaluated using 14-day-old seedlings after 15-day exposure to 75 mM NaCl in a hydroponics culture system. Salt-induced changes plant growth and morphology, photosynthetic capacity, cell membrane integrity, cellular protection enzyme systems as well other physiological biochemical traits investigated identify genotypic variability salt response. This study also analyzed heredity parameters correlation the response index (SRI)....
Accurate and efficient genotyping of simple sequence repeats (SSRs) constitutes the basis SSRs as an effective genetic marker with various applications. However, existing methods for SSR suffer from low sensitivity, accuracy, efficiency high cost. In order to fully exploit potential marker, we developed a novel method genotyping, named AmpSeq-SSR, which combines multiplexing polymerase chain reaction (PCR), targeted deep sequencing comprehensive analysis. AmpSeq-SSR is able genotype...
Histone acetylation/deacetylation is an important chromatin modification for epigenetic regulation of gene expression. Silent information regulation2 (Sir2)-related sirtuins are nicotinamide-adenine dinucleotide (NAD(+))-dependent histone deacetylases (HDAC). The mammalian sirtuin family comprises 7 members (SIRT1-7) that act in different cellular compartments to regulate metabolism and aging. rice genome contains only two Sir2-related genes: OsSRT1 (or SRT701) OsSRT2 (orSRT702). closely...
Abstract Rice bacterial blight (BB) is a devastating rice disease. The Xa21 gene confers broad and persistent resistance against BB. We introduced into Oryza sativa L ssp indica (rice 9311), through multi-generation backcrossing generated nearly isogenic, blight-resistant 9311/ rice. Using next-generation sequencing, we profiled the transcriptomes of both varieties before within four days after infection bacterium Xanthomonas oryzae pv. . identified differentially expressed (DE) genes...
Small noncoding RNA (sncRNA), including microRNAs (miRNAs) and endogenous small-interfering RNAs (endo-siRNAs) are key gene regulators in eukaryotes, playing critical roles plant development stress tolerance. Trans-acting siRNAs (ta-siRNAs), which secondary triggered by miRNAs, from natural antisense transcripts (nat-siRNAs) two well-studied classes of endo-siRNAs.In order to understand sncRNAs' chilling response acclimation, we performed a comprehensive study miRNAs endo-siRNAs Cassava...
Stress acclimation is an effective mechanism that plants acquired for adaption to dynamic environment. Even though generally considered be sensitive low temperature, Cassava, a major tropical crop, can tolerant much lower temperature after chilling acclimation. Improvement resistance could beneficial breeding. However, the underlying and effects of on tolerance remain largely unexplored.In order understand acclimation, we profiled analyzed transcriptome microRNAome using high-throughput deep...
Lead (Pb), a heavy metal, has become crucial pollutant in soil and water, causing not only permanent irreversible health problems, but also substantial reduction crop yields.In this study, we conducted proteome analysis of the roots non-hyperaccumulator inbred maize line 9782 at four developmental stages (0, 12, 24, 48 h) under Pb pollution using isobaric tags for relative absolute quantification technology.A total 252, 72 116 proteins were differentially expressed between M12 (after 12-h...
Formed by back splicing or fusion of linear RNAs, circular RNAs (circRNAs) constitute a new class non-coding eukaryotes. Recent studies reveal spliceosome-dependent biogenesis circRNAs where arise at the intron-exon junctions mRNAs. In this study, using novel de novo identification method, we show that can originate from interior regions exons, introns, and intergenic transcripts in human, mouse rice, which were referred to as (i-circRNAs). Many i-circRNAs have some remarkable...
Traditional Chinese medicine (TCM) has been practiced for thousands of years, although concerns about the efficacy, legality, and safety TCM continue to be raised. Chromatographic studies have detected presence heavy metals plant toxins within some preparations. However, chromatography is not able identify all compounds TCM, particularly those items that are clearly labeled on packaging. The present study aimed establish a supplemental method better assesses ingredient components...
Significance Transcriptional regulation of flagellar genes controls an initial step in assembly. In this study, we show that XAP5, a conserved protein unknown function, defines class transcription factor for transcriptional involved Phosphorylation X chromosome-associated 5 (XAP5) during regeneration tracks length. Remarkably, recruitment RNA polymerase II (Pol II) machinery activation depends on the activities XAP5. Our data demonstrate XAP5 functions as through Pol II. results enhance our...
Abstract Background Arundo donax L. has great potential as an energy crop due to its high biomass yield and broad adaptability, while lack of a reference genome is hindrance genetic improvement efforts for this species. Methods Genome assembly A. was conducted by utilizing PacBio SMRT sequencing high‐throughput chromosome conformation capture technology, with further analysis exploring the plant's ploidy, whole‐genome duplication event, evolutionary history through comparative genomics....
Chlorellacean members are common in aquatic or subaerial habitats, and many of them have significant economic value. Taxonomic reports organelle genome data for the
Genes with indispensable functions are identified as essential; however, the traditional gene-level studies of essentiality have several limitations. In this study, we characterized gene from a new perspective protein domains, independent structural or functional units polypeptide chain.To identify such essential developed an Expectation-Maximization (EM) algorithm-based Essential Domain Prediction (EDP) Model. With simulated datasets, model provided convergent results given different...
With 6 figures and 4 tables Abstract Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. ( Xoo ) is a major production constraint in commercial hybrid because most the parental lines used are susceptible to . In this study, cloned BB resistance gene Xa21 was transferred into ‘D62B’, maintainer line three‐line indica using double right‐border vector pDRBXa21 through an Agrobacterium tumefaciens‐ mediated system. Molecular analysis revealed that integrated genome transgenic...
Host genetic backgrounds affect gene functions. The of genetically engineered organisms must be identified to confirm their identity with those recipients. Marker-assisted backcrossing (MAB), transgenesis and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) editing are three commonly used engineering techniques. However, methods for background screening between corresponding recipients suffer from low efficiency, accuracy or high...
Increasing focus has come to the role of extracellular vesicles (EVs) in various cancers. Hence, we designed this study explore mechanism whereby microRNA-342-3p (miR-342-3p)-containing EVs derived from BMSCs might affect breast cancer. MCF-7 cancer cell line was co-incubated with isolated rat BMSCs, followed by alteration miR-342-3p and INHBA expression. Microarray-based analyses predicted a possible regulatory involving miR-342-3p, INHBA, IL13Rα2 cancer, which verified luciferase reporter,...
Popular knowledge of the population structure and genetic diversity a plant species is essential for designing improvement strategies. The genotyping-by-sequencing (GBS) approach has been used to simplify complex genomes become popular high-throughput molecular tool selecting breeding many crop plants, including those with large genomes. This study aimed construct core collection walnut (Juglans regia) germplasm using GBS approach. A panel 87 initial genotypes, 25 landraces, 12 cultivars, 50...