A5089581410- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Fluoride Effects and Removal
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- Environmental DNA in Biodiversity Studies
- Molecular Biology Techniques and Applications
- Plant nutrient uptake and metabolism
- Arsenic contamination and mitigation
- Plant responses to water stress
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Aquatic Ecosystems and Phytoplankton Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Microtubule and mitosis dynamics
- Horticultural and Viticultural Research
- Antioxidant Activity and Oxidative Stress
- Metalloenzymes and iron-sulfur proteins
- Bone and Dental Protein Studies
- Gene expression and cancer classification
- Folate and B Vitamins Research
- Silicon Effects in Agriculture
Yale University
2009-2024
Molecular Research Institute
2023
Peking University
2009
UConn Health
1989-1991
Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, tacking selected to an adhesive film with laser beam. Harvested provide DNA, RNA, and protein for profiling of genomic characteristics, gene expression, spectra cell types. We have optimized LCM variety plant tissues species, permitting harvesting paraffin that maintain histological detail. show RNA extracted LCM-harvested in amount...
C 4 and CAM photosynthesis have repeatedly evolved in plants over the past 30 million years. Because both repurpose same set of enzymes but differ their spatial temporal deployment, they long been considered as distinct incompatible adaptations. Portulaca contains multiple species that perform when droughted. Spatially explicit analyses gene expression reveal systems are completely integrated oleracea , with carbon fixation occurring cells CAM-generated metabolites likely incorporated...
The comparison of the cell-specific transcriptomes bundle sheath (BS) and mesophyll (M) cells from successive developmental stages maize (Zea mays) leaves reveals that number genes preferentially transcribed in one cell type or other varies considerably sink–source transition to mature photosynthetic stages. differentially expressed (DE) is maximal at a stage well before full maturity, including those encode key functions for C4 photosynthesis. dynamics BS/M differential expression can be...
After mating, hypotrichous ciliated protozoa transform a set of their micronuclear chromosomes into thousands short, linear DNA molecules that form the macronuclear genome. To examine genome organization in hypotrich Euplotes crassus, we have analyzed two cloned segments as well are derived from them. E. crassus was found to display number features characteristic other genomes, including (i) clustering and close spacing precursors molecules, (ii) frequent occurrence internal eliminated...
Abstract Fluoride is everywhere in the environment, yet it toxic to living things. How biological organisms detoxify fluoride has been unknown until recently. Fluoride-specific ion transporters both prokaryotes (Fluoride channel; Fluc) and fungi Exporter; FEX) efficiently export extracellular environment. FEX homologs have identified throughout plant kingdom. Understanding function of a multicellular organism will reveal valuable knowledge about reducing effects caused by fluoride. Here, we...
The fluoride export protein (FEX) in yeast and other fungi provides tolerance to (F-), an environmentally ubiquitous anion. FEX efficiently eliminates intracellular that otherwise would accumulate at toxic concentrations. homolog bacteria, Fluc, is a 'double-barreled' channel formed by dimerization of two identical or similar subunits. eukaryotes monomer resulting from covalent fusion the As result, both potential pores are created different parts same protein. Here we identify proteins...
A cytosolic NADP-malic enzyme (CYTME) has been described previously in several plants, all C3 species. CYTME is distinct from the chloroplastic (CHLME) that highly active C4 We show at least one CytMe gene present Flaveria spp., including C3, C4, and C3-C4 intermediate types. Based on expression patterns pringlei (C3) trinervia (C4), we suggest roles, supplying of NADPH for metabolism, supporting wound response or repair, balancing cellular pH illuminated leaves. These three roles are likely...
Journal Article Internal eliminated sequenc are removed prior to chromosome fragmentation during development in Euplotes crassus Get access S.Lorraine Tausta, Tausta Department of Biochemistry, University Connecticut Health CenterFarmington, CT 06032, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Lawrence A. Klobutcher Nucleic Acids Research, Volume 18, Issue 4, 25 February 1990, Pages 845–853, https://doi.org/10.1093/nar/18.4.845 Published: 1990 history...
The enzyme violaxanthin de-epoxidase (VDE) catalyzes the conversion of (V) to antheraxanthin (A) and zeaxanthin (Z). It has been purified 194-fold with a yield 1.4% from sonicate thylakoids Spinacea oleracea specific activity 19 μmol Z + A/min per mg protein. Purification steps included chromatography on DEAE-Sephadex, hydrophobic interaction Butyl Sepharose, isoelectric-focusing, gel filtration Sephadex G-100. A single peptide band 43.3 kDa was detected SDS-PAGE, apparent molecular mass...
Following the sexual phase of its life cycle, hypotrichous dilate Euplotes crassus transforms a copy chromosomal mlcronucleus into transcriptionally active macronucleus containing short, linear, gene-sized DNA molecules. Tens thousands breakage and joining, or splicing, events occur during macronuclear development. The removed by such includes transposon-like elements, referred to as Tec1 well segments unique sequence DNA, termed internal eliminated sequences (lESs). Both types elements are...
After mating, hypotrichous ciliated protozoa transform a set of their micronuclear chromosomes into thousands short, linear DNA molecules that form the macronuclear genome. To examine genome organization in hypotrich Euplotes crassus, we have analyzed two cloned segments as well are derived from them. E. crassus was found to display number features characteristic other genomes, including (i) clustering and close spacing precursors molecules, (ii) frequent occurrence internal eliminated...
Abstract Fluoride is an environmental toxin prevalent in water, soil, and air. A fluoride transporter called EXporter (FEX) has been discovered across all domains of life, including bacteria, single cell eukaryotes, plants, that required for tolerance. How FEX functions to protect multicellular plants unknown. In order distinguish between different models, the dynamic movement wildtype (WT) fex mutant was monitored using [ 18 F]fluoride with positron emission tomography. Significant...
ABSTRACT C 4 grasses often outperform 3 species under hot, arid conditions due to superior water and nitrogen use efficiencies lower rates of photorespiration. A method concentrating CO 2 around the site carbon fixation in bundle sheath (BS) is required realize these gains. In NADP-malic enzyme (NADP-ME)-type such as maize, suberin deposition BS cell wall hypothesized act a diffusion barrier escape O entry from surrounding mesophyll cells. Suberin heteropolyester comprised acyl-lipid-derived...
Summary C 4 and CAM photosynthesis have repeatedly evolved in plants over the past 30 million years. Because both repurpose same set of enzymes but differ their spatial temporal deployment, they long been considered as distinct incompatible adaptations. Remarkably, Portulaca contains multiple species that perform when droughted. Spatially explicit analyses gene expression reveal systems are completely integrated P. oleracea , with carbon fixation occurring cells CAM-generated metabolites...
Fluorine is the 13th-most abundant element on earth, found most often bound to other elements in its negatively charged form, fluoride. Fluoride compounds are used improve dental health, make steel, and useful materials like Teflon. also emitted into environment as a byproduct of both natural industrial processes. even contaminates fertilizer help plants grow. In high amounts, fluoride can be toxic. Single-celled organisms bacteria protect themselves by making transporter that specifically...