A5038778306- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Biofuel production and bioconversion
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Enzyme Production and Characterization
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Plant Parasitism and Resistance
- Lysosomal Storage Disorders Research
- Polysaccharides Composition and Applications
- Sphingolipid Metabolism and Signaling
- Food composition and properties
- Galectins and Cancer Biology
- Microbial Metabolites in Food Biotechnology
- Lipid metabolism and biosynthesis
- Agronomic Practices and Intercropping Systems
- Potato Plant Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
Joint BioEnergy Institute
2010-2021
Lawrence Berkeley National Laboratory
2013-2021
University of California, Berkeley
2009-2018
University of Nebraska–Lincoln
2016-2018
Sandia National Laboratories California
2012
Agriaquaculture Nutritional Genomic Center
2009
Cornell University
2009
Mechanisms of phloem loading in the minor veins leaves are known for only a few species. We propose that there limited number strategies primary photoassimilates, sucrose and sugar alcohols. These can be predicted based on thermodynamic anatomical considerations identified by autoradiography following uptake (14)C-labeled compounds, analysis leaf solute composition concentrations, plasmodesmatal counting. Experiments 45 dicotyledonous species patterns. Over 50-fold differences concentrations...
Xylan is a major component of the plant cell wall and most abundant noncellulosic in secondary walls that constitute largest part biomass. Dicot glucuronoxylan consists linear backbone β(1,4)-linked xylose residues substituted with α(1,2)-linked glucuronic acid (GlcA). Although several genes have been implicated xylan synthesis through mutant analyses, biochemical mechanisms responsible for synthesizing are largely unknown. Here, we show evidence activity GUX1 (for GlcA substitution 1),...
Abstract β-1,4-Galactans are abundant polysaccharides in plant cell walls, which generally found as side chains of rhamnogalacturonan I. Rhamnogalacturonan I is a major component pectin with backbone alternating rhamnose and galacturonic acid residues that include α-1,5-arabinans, β-1,4-galactans, arabinogalactans. Many enzymes required to synthesize pectin, but few have been identified. Pectin most primary walls expanding cells, β-1,4-galactan relatively secondary especially tension wood...
Xylans constitute the major non-cellulosic component of plant biomass. Xylan biosynthesis is particularly pronounced in cells with secondary walls, implying that synthesis network consists a set highly expressed genes such cells. To improve understanding xylan biosynthesis, we performed comparative analysis co-expression networks between Arabidopsis and rice as reference species different wall types. Many co-expressed were represented by orthologs both species, which implies common...
Abstract Glycosyl inositol phosphorylceramide (GIPC) sphingolipids are a major class of lipids in fungi, protozoans, and plants. GIPCs abundant the plasma membrane plants, comprising around quarter total these membranes. Plant contain unique glycan decorations that include conserved glucuronic acid (GlcA) residue various additional sugars; however, no proteins responsible for glycosylating have been identified to date. Here, we show Arabidopsis thaliana protein INOSITOL PHOSPHORYLCERAMIDE...
Glycosylinositol phosphorylceramides (GIPCs) are a class of glycosylated sphingolipids found in plants, fungi, and protozoa. These lipids abundant the plant plasma membrane, forming ∼25% total membrane lipids. Little is known about function headgroup, but two recent studies have indicated that they play key role signaling defense. Here, we show member glycosyltransferase family 64, previously named ECTOPICALLY PARTING CELLS1, likely Golgi-localized GIPC-specific mannosyl-transferase, which...
Many plants translocate sugar alcohols in the phloem. However, mechanism(s) of alcohol loading minor veins leaves are debated. We characterized strategies two species that transport sorbitol (Plantago major and apple [Malus domestica]), one transports mannitol (Asarina scandens). Plasmodesmata abundant at all interfaces vein phloem apple, types A. scandens. Few plasmodesmata present P. major. Apple differs from other sucrose (Suc) much higher concentrations leaves. leaf tissue exposed to...
Summary Sphingolipids are a major component of plant plasma membranes and endomembranes, mediate diverse range biological processes. Study the highly glycosylated glycosyl inositol phosphorylceramide ( GIPC ) sphingolipids has been slow as result challenges associated with extractability s, their functions in remain poorly characterized. We recently discovered an Arabidopsis glucuronosyltransferase, INOSITOL PHOSPHORYLCERAMIDE GLUCURONOSYLTRANSFERASE 1 IPUT 1), which is first enzyme...
Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to glycan headgroup of varying structures, are the major sphingolipids in plant plasma membrane. Recently, we identified biosynthetic genes for GIPC glycosylation Arabidopsis (Arabidopsis thaliana) and demonstrated that is essential viability. However, function GIPCs significance their structural variation poorly understood. Here, characterized glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE...
Pectin is a major component of primary cell walls and performs plethora functions crucial for plant growth, development plant-defense responses. Despite the importance pectic polysaccharides their biosynthesis poorly understood. Several genes have been implicated in pectin by mutant analysis, but biochemical activity has shown very few. We used reverse genetics analysis to study members Glycosyltransferase Family 92 (GT92) Arabidopsis thaliana. Biochemical gave detailed insight into...
Plant endosymbiosis relies on the development of specialized membranes that encapsulate endosymbiont and facilitate nutrient exchange. However, identity function lipids within these membrane interfaces is largely unknown. Here, we identify GLUCOSAMINE INOSITOL PHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) as a sphingolipid glycosyltransferase highly expressed in Medicago truncatula root nodules roots colonized by arbuscular mycorrhizal (AM) fungi further demonstrate this enzyme functions synthesis...