A5100638355- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Cellular transport and secretion
- Environmental and Agricultural Sciences
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Calcium signaling and nucleotide metabolism
- Plant Stress Responses and Tolerance
- Forest, Soil, and Plant Ecology in China
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Insect Resistance and Genetics
- RNA regulation and disease
- Insect-Plant Interactions and Control
University of California, San Diego
2012-2021
Cornell University
2017-2018
Shanghai Public Health Clinical Center
2014
Institute of Genetics and Developmental Biology
2007-2013
State Key Laboratory of Plant Genomics
2007-2013
University of Chinese Academy of Sciences
2010-2013
Chinese Academy of Sciences
2007-2013
Auxin plays an essential role in root development. It has been a long-held dogma that auxin required for development is mainly transported from shoots into roots by polarly localized transporters. However, it known also synthesized roots. Here we demonstrate group of YUCCA (YUC) genes, which encode the rate-limiting enzymes biosynthesis, Arabidopsis Five YUC genes (YUC3, YUC5, YUC7, YUC8 and YUC9) display distinct expression patterns during Simultaneous inactivation five (yucQ mutants) leads...
Auxin regulates every aspect of plant growth and development. Previous genetic studies demonstrated that YUCCA (YUC) flavin-containing monooxygenases (FMOs) catalyze a rate-limiting step in auxin biosynthesis YUCs are essential for many developmental processes. We proposed convert indole-3-pyruvate (IPA) to indole-3-acetate (IAA). However, the exact biochemical mechanism has remained elusive. Here we present characterization recombinant Arabidopsis YUC6. Expressed purified from Escherichia...
The development and growth of higher plants is highly dependent on the conduction water minerals throughout plant by xylem vessels. In Arabidopsis roots organized as an axis cell files with two distinct fates: central metaxylem peripheral protoxylem. During vascular development, high low expression levels class III HD-ZIP transcription factors promote protoxylem identities, respectively. Protoxylem specification determined both mobile, ground tissue-emanating miRNA165/6 species, which...
In planta RNAi against essential insect genes offers a promising route to control crop pests, but is constrained for many groups, notably phloem sap-feeding hemipterans, by poor efficacy. This study conducted on the phloem-feeding whitefly Bemisia tabaci reared tomato plants investigated causes of low efficacy and routes ameliorate problem. Experiments using transgenic lines containing ds-GFP (green fluorescent protein) revealed that full-length dsRNA phloem-mobile, ingested insects,...
The eukaryotic translation initiation factor 5A (eIF-5A) is a highly conserved protein found in all organisms. Although originally identified as factor, recent studies mammalian and yeast (Saccharomyces cerevisiae) cells suggest that eIF-5A mainly involved RNA metabolism trafficking, thereby regulating cell proliferation, growth, programmed death. In higher plants, the physiological function of remains largely unknown. Here, we report identification characterization an Arabidopsis...
Flowering is triggered by the transmission of a mobile protein, FLOWERING LOCUS T (FT), from leaves to shoot apex. FT originates in phloem leaf veins. However, identity FT-synthesizing cells not known. As result, it has been possible determine whether complex regulatory networks that control synthesis involve intercellular communication, as case many aspects plant development. We demonstrate here Arabidopsis thaliana and orthologs Maryland Mammoth tobacco (Nicotiana tabacum) are produced two...
Abstract Auxin is a key regulator of plant growth and development. Local auxin biosynthesis intercellular transport generates regional gradients in the root that are instructive for processes such as specification developmental zones maintain tropic responses. Here we present toolbox to study auxin-mediated development features: (i) ability control synthesis with high spatio-temporal resolution (ii) single-cell nucleus tracking morphokinetic analysis infrastructure. Integration these two...
Abstract The phytohormone cytokinin regulates various aspects of plant growth and development, including root vascular development. In Arabidopsis thaliana, mutations in the signaling components cause misspecification protoxylem cell files. Auxin antagonizes cytokinin-regulated differentiation by inducing expression ARABIDOPSIS PHOSPHOTRANSFER PROTEIN6 (AHP6), a negative regulator signaling. However, molecular mechanism is not fully understood. Here, we show that mutation FUMONISIN...
Internalization and sorting of macromolecules are inherent properties all eukaryotic cells that achieved by vesicle trafficking. However, this process is relatively less understood in plants. An eight-subunit protein complex, BLOC-1, which involved endosomal transport from the endosomes to lysosomes, has been identified both human mice. In study, two homologous subunits BLOS1 (or AtGCN5L1) BLOS2, have characterized Arabidopsis. Both BLOS2 interacted with SNX1 on endosomes. Inducible RNAi...
To investigate the histopathological manifestations of two fatal cases H7N9 influenza A virus infection.Pulmonary and hepatic specimens from infection were examined using H&E staining. Additionally, in situ hybridisation was performed with probes (ViewRNA) targeting RNA IP-10, interleukin (IL)-6 mRNA. The distribution surfactant protein (SP-A), B (SP-B), CD3, CD4, CD8, CD68 C4d determined immunohistochemistry. Apart typical diffuse alveolar damage hyaline membrane observed severe infection,...
YUC flavin monooxygenases catalyze the rate-limiting step of auxin biosynthesis. Here we report vacuolar targeting and degradation GFP-YUC1. GFP-YUC1 fusion expressed in Arabidopsis protoplasts or transgenic plants was primarily localized vacuoles. Surprisingly, established that GFP-YUC1, a soluble protein, sorted to vacuoles through ESCRT pathway, which has long been recognized for sorting integral membrane proteins. We further show ubiquitinated this form targeted degradation, process also...