A5072956402- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Clostridium difficile and Clostridium perfringens research
- Pneumocystis jirovecii pneumonia detection and treatment
- Animal Disease Management and Epidemiology
- HIV/AIDS oral health manifestations
- Viral-associated cancers and disorders
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Antifungal resistance and susceptibility
- HIV/AIDS Research and Interventions
- Viral gastroenteritis research and epidemiology
- Inflammatory Bowel Disease
- RNA and protein synthesis mechanisms
- Fungal Infections and Studies
Shanghai Public Health Clinical Center
2014-2021
Fudan University
2014-2020
Shanghai Medical College of Fudan University
2020
Huashan Hospital
2014
Abstract Metagenomic next-generation sequencing (mNGS) holds promise as a diagnostic tool for unbiased pathogen identification and precision medicine. However, its medical utility depends largely on assay simplicity reproducibility. In the current study, we aimed to develop streamlined Illumina Oxford Nanopore-based DNA/RNA library preparation protocol rapid data analysis pipeline. The sequencing-based mNGS method was first developed evaluated using set of samples with known aetiology. Its...
Candida albicans, as an opportunistic pathogen, can cause superficial and life-threatening candidiasis in immunocompromised individuals. The formation of surface-associated biofilms the appearance drug resistance pose a significant challenge for clinical intervention. In this study, total 104 hospital-acquired C. alibcans isolates were collected from sterile sites mucosal lesions 92 infectious disease patients Shanghai Public Health Clinical Center analysed. rates to fluconazole,...
To investigate the histopathological manifestations of two fatal cases H7N9 influenza A virus infection.Pulmonary and hepatic specimens from infection were examined using H&E staining. Additionally, in situ hybridisation was performed with probes (ViewRNA) targeting RNA IP-10, interleukin (IL)-6 mRNA. The distribution surfactant protein (SP-A), B (SP-B), CD3, CD4, CD8, CD68 C4d determined immunohistochemistry. Apart typical diffuse alveolar damage hyaline membrane observed severe infection,...
Abstract Aims Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient COVID-19. Methods The was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram Ziehl–Neelsen stain and Grocott’s Methenamine Silver (GMS) Stain. distribution CD3, CD4, CK20 CD68, cytomegalovirus (CMV) Herpes Simplex Virus (HSV) antigen were determined immunohistochemistry. In situ hybridization (ISH) SARS-CoV-2...
Abstract Metagenomic next generation sequencing (mNGS) holds promise as a diagnostic tool for unbiased pathogen identification and precision medicine. However, its medical utility depends largely on the assay simplicity reproducibility. In current study, we aimed to develop streamlined Illumina Oxford Nanopore-based DNA/RNA library preparation protocol rapid data analysis pipeline. The based mNGS method was first developed evaluated using set of samples with known etiology. Its sensitivity...