- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Crop Yield and Soil Fertility
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Sugarcane Cultivation and Processing
- Plant Reproductive Biology
- Genetics and Plant Breeding
- Biofuel production and bioconversion
- 14-3-3 protein interactions
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Plant responses to water stress
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Gene expression and cancer classification
- Seed Germination and Physiology
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Plant Micronutrient Interactions and Effects
- Trace Elements in Health
- Single-cell and spatial transcriptomics
- Bioinformatics and Genomic Networks
Guangdong Academy of Agricultural Sciences
2024-2025
Guangdong Academy of Sciences
2020-2024
China Agricultural University
2015-2023
Guangdong Provincial Academy of Environmental Science
2022
South China Agricultural University
2022
Key Laboratory of Guangdong Province
2022
Zhongkai University of Agriculture and Engineering
2021
Gansu Academy of Sciences
2010
Lanzhou University
2008
Lanzhou University Second Hospital
2008
Drought is one of the major factors limiting global maize production. Exposure to long-term drought conditions inhibits growth and leads yield losses. Although several drought-responsive genes have been identified functionally analyzed, mechanisms underlying responses water recovery treatments not fully elucidated. To characterize how seedling respond stress at transcriptional level, we analyzed physiological differentially expressed (DEGs) in inbred line B73 under deficit conditions.The...
The early maize (Zea mays) seed undergoes several developmental stages after double fertilization to become fully differentiated within a short period of time, but the genetic control this highly dynamic and complex process remains largely unknown. Here, we report high temporal-resolution investigation transcriptomes using 31 samples collected at an interval 4 or 6 h first six days development. These time-course were clearly separated into four distinct groups corresponding fertilization,...
Abstract Broomcorn millet ( Panicum miliaceum L.) has strong tolerance to abiotic stresses, and is probably one of the oldest crops, with its earliest cultivation that dated back ca . ~10,000 years. We report here genome assembly through a combination PacBio sequencing, BioNano, Hi-C (in vivo) mapping. The 18 super scaffolds cover ~95.6% estimated (~887.8 Mb). There are 63,671 protein-coding genes annotated in this tetraploid genome. About ~86.2% syntenic foxtail have two homologous copies...
Abstract Long-range chromatin interactions are important for transcriptional regulation of genes, many which related to complex agronomics traits. However, the pattern three-dimensional remains unclear in plants. Here we report generation interaction analysis by paired-end tag sequencing (ChIA-PET) data and construction extensive H3K4me3- H3K27ac-centered maps maize. Results show that interacting patterns between proximal distal regulatory regions genes highly dynamic. Genes with have higher...
Circadian clocks are endogenous auto-regulatory mechanisms that allow organisms, from bacteria to humans, advantageously time a wide range of activities within 24 h environmental cycles. Here we report the identification and characterization an MYB-related gene, designated 1 (CIR1), is involved in circadian regulation Arabidopsis. Expression CIR1 transiently induced by light oscillates with rhythm. The rhythmic expression controlled central oscillator. Constitutive resulted shorter period...
VQ motif-containing proteins play crucial roles in abiotic stress responses plants. Recent studies have shown that some physically interact with WRKY transcription factors to activate downstream genes. In the present study, we identified and characterized genes encoding using most recent version of maize genome sequence. total, 61VQ were identified. a cluster analysis, these clustered into nine groups together their homologous rice Arabidopsis. Most (57 out 61 numbers) found be single-copy...
Summary The number of leaves and their distributions on plants are critical factors determining plant architecture in maize ( Zea mays ), leaf is frequently used as a measure flowering time, trait that key to local environmental adaptation. Here, using large set 866 maize‐teosinte BC 2 S 3 recombinant inbred lines genotyped by 19 838 single nucleotide polymorphism markers, we conducted comprehensive genetic dissection assess the its relationship time. We demonstrated two components total...
The root system is fundamental for maize growth and yield. Characterizing its heterogeneity cell type-specific response to nitrate at the single-cell level will shed light on development nutrient uptake. We profiled transcriptomes of >7000 cells derived from tips seedlings grown media with or without nitrate, identified 11 major types tissues 85 nitrate-response genes, including several known metabolic genes. A pseudotime analysis showed a continuous series beginning meristematic zone that...
SUMMARY Sugarcane is the main source of sugar worldwide, and 80% sucrose production comes from sugarcane. However, genetic differentiation basis agronomic traits remain obscure. Here, we sequenced whole‐genome 219 elite worldwide sugarcane cultivar accessions. A total approximately 6 million high‐quality genome‐wide single nucleotide polymorphisms (SNPs) were detected. association study identified a 2198 SNPs that significantly associated with content, stalk number, plant height, diameter,...
Cadmium is a toxic, carcinogenic element that threatens food safety due to its tendency be absorbed by plants along with essential nutrients. This study conducted genome-wide association (GWAS) using SNP genotyping data from 170 natural maize populations analyze cadmium content in grains across three environments. The MLM_Q+Kinship and MLM_PCA+Kinship models identified 6424 (HN), 991 (JMO), 1358 (JMT) SNPs linked accumulation the model, 121 common all Additionally, model detected 824 950 910...
Abstract The MYB transcription factor genes play important roles in many developmental processes and various defense responses of plants. shikimate pathway is a major biosynthetic for the production three aromatic amino acids other compounds that are involved multiple plants, including protection against UV defense. Herein, we describe characterization R2R3‐MYB gene AtMYB15 as an activator Arabidopsis. protein nuclear localized transcriptional activation domain found its C‐terminal portion....
Abstract Carbon and nitrogen are the two main nutrients in maize (Zea mays L.) kernels, kernel filling metabolism determine seed formation germination. However, molecular mechanisms underlying relationship between corresponding carbon remain largely unknown. Here, we found that HEAT SHOCK PROTEIN 90.6 (HSP90.6) is involved both processes of nitrogen. A single-amino acid mutation within HATPase_c domain HSP90.6 led to small kernels. Transcriptome profiling showed expression amino...
The complex interactions between transcription factors (TFs) and their target genes in a spatially temporally specific manner are crucial to all cellular processes. Reconstruction of gene regulatory networks (GRNs) from expression profiles can help decipher TF-gene regulations variety contexts; however, the inevitable prediction errors GRNs hinder optimal data mining RNA-Seq transcriptome profiles. Here we perform an integrative study Zea mays (maize) seed development order identify key...
Abstract Background Maize kernel colour is an important index for evaluating maize quality and value mainly entails two natural pigments, carotenoids anthocyanins. To analyse the genetic mechanism of mine single nucleotide polymorphisms (SNPs) related to traits, association panel including 244 superior inbred lines was used measure six traits in environments then combined with about 3 million SNPs covering whole genome this study. Two models (Q + K, PCA K) were genome-wide analysis (GWAS)...
Abstract Maize ( Zea mays L.), a staple food and significant economic crop, is enriched with riboflavin, micronutrients other compounds that are beneficial for human health. As emphasis on the nutritional quality of crops increases maize research has expanded to focus both yield quality. This study exploreed genetic factors influencing micronutrient levels in kernels through comprehensive genome-wide association (GWAS). We utilized diverse panel 244 inbred lines approximately 3 million...
Abstract Background As maize originated in tropical or subtropical zones, most germplasm is extremely sensitive to low temperatures during the seedling stage. Clarifying molecular mechanism of cold acclimation would facilitate breeding tolerant varieties, which one major sustainability factors for crop production. To meet this goal, we investigated two inbred lines with contrasting levels tolerance at stage (IL85, a line; B73, line), and performed full-length transcriptome sequencing on root...
Pentatricopeptide repeat (PPR) proteins are a large protein family in higher plants and play important roles during seed development. Most reported PPR function mitochondria. However, some localize to more than one organelle; functional characterization of these remains limited maize (Zea mays L.). Here, we cloned analyzed the P-subfamily protein, PPR278. Loss-function PPR278 led lower germination rate other defects at seedling stage, as well smaller kernels compared wild type. was expressed...
Maize (Zea mays L.) is an important food and feed crop worldwide serves as a vital source of biological trace elements, which are breeding targets. In this study, 170 maize materials were used to detect QTNs related the content Mn, Fe Mo in grains through two GWAS models, namely MLM_Q + K MLM_PCA K. The results identified 87 (Mn), 205 (Fe), 310 (Mo) using both methods three environments. Considering comprehensive factors such co-location across multiple environments, strict significance...
Abstract Uncovering the genetic basis of seed development will provide useful tools for improving both crop yield and nutritional value. However, regulatory networks maize ( Zea mays ) remain largely unknown. The opaque endosperm small germ 1 os1 mutant has a embryo. Here, we cloned OS1 show that it encodes putative transcription factor containing an RWP‐RK domain. Transcriptional analysis indicated expression is elevated in early development, especially basal transfer layer (BETL),...
Sugarcane ( Saccharum spp.) is an efficient crop mainly used for sugar and bioethanol production. High yield high sucrose of sugarcane are always the fundamental demands in growth worldwide. Leaf angle size can be attributed to planting density, which was associated with yield. In this study, we performed genome-wide association studies (GWAS) a panel 216 core parents their derived lines (natural population) determine genetic basis leaf key candidate genes +2, +3, +4 at seedling, elongation,...
In this study, the contents of four carotenoids in 244 maize inbred lines were detected and about three million single nucleotide polymorphisms (SNPs) for genome-wide association study to preliminarily analyze genetic mechanism kernel carotenoids. We identified 826 quantitative trait loci (QTLs) significantly associated with contents, two key candidate genes Zm00001d029526 (CYP18) Zm00001d023336 (wrky91) obtained. addition, we found a germplasm IL78 higher The results can provide theoretical...