- Wheat and Barley Genetics and Pathology
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Phytase and its Applications
- Food composition and properties
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Primate Behavior and Ecology
- Agricultural Productivity and Crop Improvement
- Hemispheric Asymmetry in Neuroscience
- Milk Quality and Mastitis in Dairy Cows
- Child and Animal Learning Development
- Mycotoxins in Agriculture and Food
- Identification and Quantification in Food
- SARS-CoV-2 and COVID-19 Research
- Crop Yield and Soil Fertility
- Probiotics and Fermented Foods
- Genetic Mapping and Diversity in Plants and Animals
- Diphtheria, Corynebacterium, and Tetanus
- Veterinary medicine and infectious diseases
- Galectins and Cancer Biology
- Escherichia coli research studies
Northeast Normal University
2024
China Agricultural University
2019-2023
Sun Yat-sen University
2023
Sixth Affiliated Hospital of Sun Yat-sen University
2023
Ministry of Agriculture and Rural Affairs
2023
Northwest A&F University
2005-2022
Northwest Institute of Mechanical and Electrical Engineering
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Beijing Chaoyang Emergency Medical Center
2021
Institute of Laboratory Animal Science
2021
The early maize (Zea mays) seed undergoes several developmental stages after double fertilization to become fully differentiated within a short period of time, but the genetic control this highly dynamic and complex process remains largely unknown. Here, we report high temporal-resolution investigation transcriptomes using 31 samples collected at an interval 4 or 6 h first six days development. These time-course were clearly separated into four distinct groups corresponding fertilization,...
Abstract Broomcorn millet ( Panicum miliaceum L.) has strong tolerance to abiotic stresses, and is probably one of the oldest crops, with its earliest cultivation that dated back ca . ~10,000 years. We report here genome assembly through a combination PacBio sequencing, BioNano, Hi-C (in vivo) mapping. The 18 super scaffolds cover ~95.6% estimated (~887.8 Mb). There are 63,671 protein-coding genes annotated in this tetraploid genome. About ~86.2% syntenic foxtail have two homologous copies...
The furin cleavage site in the spike glycoprotein of SARS-CoV-2 coronavirus is considered important for virus to enter host cells. By analyzing 45828 genome sequences, we identified 103 strains with various DNA mutations including 18 unique non-synonymous point mutations, one deletion, and six gains premature stop codon that may affect site. Our results revealed might not be required human cells <italic>in vivo</italic>. mutants represent a new subgroup reduced tropism transmissibility as...
With the continued spread of SARS-CoV-2 virus around world, researchers often need to quickly identify novel mutations in newly sequenced genomes for studying molecular evolution and epidemiology virus. We have developed a Python package, MicroGMT, which takes either raw sequence reads or assembled genome sequences as input compares against database characterize indels point mutations. Although our default setting is optimized virus, package can be also applied any other microbial genomes....
Wheat (Triticum aestivum L.) is one of the most important crops cultivated worldwide. Identifying complete transcriptome wheat grain could serve as foundation for further study seed development. However, relatively large size and polyploid complexity genome have been substantial barriers to molecular genetics analysis wheat. Alternatively, RNA sequencing has provided some useful information about genes. because number short reads generated by sequencing, factors that are crucial assembly,...
Grain development is one of the biological processes, which contributes to final grain yield. To understand molecular changes taking place during early development, we profiled proteomes two common wheat cultivars P271 and Chinese Spring (CS) with large small grains, respectively at three developmental stages (4, 8, 12 days post anthesis). An iTRAQ (isobaric tags for relative absolute quantitation) based proteomics approach was used this purpose. More than 3,600 proteins were reported...
Phospholipid-hydrolyzing enzymes include members of the phospholipase C (PLC) family that play important roles in regulating plant growth and responding to stress. In present study, a systematic silico analysis wheat PLC gene revealed total 26 genes (TaPLCs). Phylogenetic sequence alignment analyses divided into 2 subfamilies, TaPI-PLC (containing typical X, Y, C2 domains) TaNPC phosphatase domain). TaPLC expression patterns differed among tissues, organs, under abiotic stress conditions....
Trueperella pyogenes is a common inhabitant of mucosal surfaces in animals and causes variety infections, including endometritis, mastitis, liver abscessation, dairy cows. Many antimicrobial agents are used for treatment infections caused by T. pyogenes; however, antibiotic resistance has recently become serious problem. The objective this study was to characterize the effect efflux pump-encoding multidrug toxic compound extrusion (MATE) gene on isolates from cows with signs endometritis. As...
Egg quality and nutritional value are becoming increasingly important to consumers, offering a new direction for the development of high-quality eggs. In this study, we conducted comprehensive analysis egg nutrient profiles in native breeds at different ages, integrating pedigree data. Our results reveal dynamic changes qualities, stronger associations among eggshell-related traits, effect onset production body weight on qualities. The heritability traits was estimated, ranging from 0.05...
Transcriptome deep sequencing (RNA-seq) has become a routine method for global gene expression profiling. However, its application to large-scale experiments remains limited by cost and labor constraints. Here we describe massively parallel 3' end RNA-seq (MP3RNA-seq) that introduces unique sample barcodes during reverse transcription permit pooling immediately following this initial step. MP3RNA-seq allows handling of hundreds samples in single experiment, at about $6 per library...
Flag leaf senescence is an important determinant of wheat yield, as occurs in a coordinated manner during grain filling. However, the biological process early flag leaves post-anthesis not clear. In this study, was investigated using high-throughput RNA sequencing technique. A total 4887 differentially expressed genes (DEGs) were identified, and any showing drastic expression changes then linked to particular processes. hierarchical cluster analysis implied potential relationships between...
Background: Crohn's disease (CD), a chronic gastrointestinal inflammatory disease, is increasing in China. With focus on Han Chinese families with CD, the aim of this study was to find genetic variations that increase CD susceptibility by genome sequencing, association, expression, and functional research. Materials methods: We performed family-based sequencing (WGS) analysis 24 patients from 12 then filtered shared potential causal variants incorporating association results meta-analyses...
Using isobaric tags for relative and absolute quantification (iTRAQ) associated analytic technologies, we have cataloged compared 7 069 unique wheat proteins expressed during four substages of the filling stage. Among them, 859 are differentially expressed, showing at least a 2-fold difference in concentration across substages. Differentially (DEPs) includind high-molecular weight glutenin subunit (W5AIU1), low-molecular (Q8W3V4), gliadin/avenin-like seed protein (D2KFG9), avenin-like...