A5100662992- Nanopore and Nanochannel Transport Studies
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Phytochemicals and Antioxidant Activities
- Electrostatics and Colloid Interactions
- SARS-CoV-2 detection and testing
- Phytochemical Studies and Bioactivities
- Diphtheria, Corynebacterium, and Tetanus
- Probiotics and Fermented Foods
- Computational Drug Discovery Methods
- Phytochemical and Pharmacological Studies
- Milk Quality and Mastitis in Dairy Cows
- Yersinia bacterium, plague, ectoparasites research
- Advanced biosensing and bioanalysis techniques
- Morinda citrifolia extract uses
- Aquaculture disease management and microbiota
- Developmental Biology and Gene Regulation
- Medicinal Plants and Bioactive Compounds
- Ultrasound and Cavitation Phenomena
- Flavonoids in Medical Research
- Advanced Battery Technologies Research
- Erythrocyte Function and Pathophysiology
- Pneumonia and Respiratory Infections
- Cancer Cells and Metastasis
- Identification and Quantification in Food
Nanjing Drum Tower Hospital
2025
University of South Carolina
2019-2023
Ruijin Hospital
2021-2023
Liaoning University of Traditional Chinese Medicine
2023
Chinese Academy of Sciences
2018-2022
Shenyang Agricultural University
2017-2022
Institute of Mechanics
2022
University of Chinese Academy of Sciences
2022
Shanghai Ocean University
2021
National Institute for Communicable Disease Control and Prevention
2021
Bubble formation plays an important role in industries concerned with mineral flotation, food, cosmetics, and materials, which requires additional energy to produce the liquid-gas interfaces. A naturally observed fact is, owing effect of surface tension, a bubble film tends retract reduce its area. Here we show "reverse" phenomenon whereby drop is transformed into using acoustic levitation via resonance. Once volume cavity encapsulated by buckled reaches critical value V*, resonance occurs...
Abstract The extensively activated Notch signaling pathway in pancreatic cancer cells is important carcinogenesis, chemoresistance, and recurrence. Targeting this a promising therapeutic strategy for cancer; however, few successful approaches have been reported, currently used molecular inhibitors of exhibit limited clinical benefits. In study, we identified previously uncharacterized microprotein, Notch1 degradation-associated regulatory polypeptide (N1DARP), encoded by LINC00261 . N1DARP...
Research on the extraction technology, component analysis, and antioxidant, antibacterial, analgesic anti-inflammatory activities of flavonoids fraction from Tribulus terrestris L. leaves was carried out. The process optimized by response surface method, maximum yield 0.27% for achieved with concentration ethanol solution 25.87%, ratio solvent to material 30:1 ml/g, ultrasonic time 27.93 min. Moreover, 14 compositions were identified separately HPLC-DAD-ESI-MS2. In addition, exhibited a...
Pluronic L61 unimers, which are biomacromolecular modulators, and curcumin, a small-molecule modulator, were co-formulated into pH-sensitive micelles to reveal the full synergistic potential of combination drug treatments reverse multidrug resistance (MDR). Compared monotherapy, combined therapy significantly improved cytotoxicity, cellular uptake apoptotic effects doxorubicin (DOX) against MCF-7/ADR cells. In mechanistic studies, both curcumin enhanced cytotoxic effect by acting on...
Abstract Despite major advances in HIV testing, ultrasensitive detection of early infection remains challenging, especially for the viral capsid protein p24, which is an virological biomarker HIV-1 infection. Here, To improve p24 patients missed by immunological tests that dominate diagnostics market, we show a click chemistry amplified nanopore (CAN) assay quantitative detection. This strategy achieves 20.8 fM (0.5 pg/ml) limit antigen human serum, demonstrating 20~100-fold higher...
Accompanied by the damages of epithelial and capillary endothelial cell, acute lung injury is diagnosed with typical pathological symptoms in clinic, including diffusing pulmonary interstitial, alveolar oedema hypoxic respiratory insufficiency. Current study focused on investigation anti-inflammatory action mechanisms total flavonoids extract (TFE) from Abutilon theophrasti Medic. leaves ALI mice induced LPSs. Mice were administrated intragastrically TFE at concentrations 0.25, 0.5, or 1.0...
Abstract Cancer biomarkers are expected to be indicative of the occurrence certain cancer diseases before tumors form and metastasize. However, many can only acquired in extremely low concentrations, which often beyond limit detection (LOD) current instruments technologies. A practical strategy for nanopore sensing raw human blood down femtomolar level is developed here. This first converts quantification copper ions by conducting a sandwich assay involving oxide nanoparticles. The released...
The objective of this study was to construct a novel strategy for the detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants using multiplex PCR-mass spectrometry minisequencing technique (mPCR-MS minisequencing). Using nucleic acid sequence SARS-CoV-2 nonvariant and synthetic variant-carrying plasmid, matrix-assisted laser desorption ionization-time flight mass (MALDI-TOF MS) method based on single-base probe extension PCR amplification products established detect 9...
Peroxisome proliferator-activated receptor gamma (PPARγ) is a key target for metabolic disorders that contribute to obesity and type 2 diabetes mellitus (T2DM). However, full agonists such as thiazolidinediones (TZDs) have limitations in terms of side effects. Selective PPARγ modulators (SPPARγMs) alternative binding pockets offer the potential safer partial agonists. Here, we employed six computational algorithms (Fpocket, DeepSite, CavityPlus, DoGSiteScorer, CASTpFold, POCASA) identify...
Nanopore technology has been employed as a powerful tool for DNA sequencing and analysis. To extend this method to peptide sequencing, necessary step is profile individual amino acids (AAs) through their nanopore stochastic signals, which remains great challenge because of the low signal-to-noise ratio unpredictable conformational changes AAs during translocation nanopores. We showed that combination an N-terminal derivatization strategy with could lead effective in situ differentiation AAs....
Nanopore technology holds remarkable promise for sequencing proteins and peptides. To achieve this, it is necessary to establish a characteristic profile each individual amino acid through the statistical description of its translocation process. However, subtle molecular differences among all twenty acids along with their unpredictable conformational changes at nanopore sensing region result in very low distinguishability. Here we report electrical using an α-hemolysin based on...
TatD DNases are conserved proteins in a variety of organisms and considered potential virulence factors Plasmodium falciparum Streptococcus pneumoniae. However, the function has not yet been determined Trueperella pyogenes, which causes various infections animals leads to economic losses. In this study, we describe roles T. pyogenes (TpTatDs). A bioinformatics analysis was performed investigate sequence characteristics TpTatDs, then ability recombinant hydrolyze DNA presence divalent...
Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling T. animal husbandry industry. Luteolin a naturally derived flavonoid compound with antibacterial properties. In this study, effects and mechanism luteolin on biofilm were analyzed explored. MBIC MBEC 156 μg/mL 312 μg/mL, respectively. also observed by confocal laser microscope scanning electron...
AMPA receptors in cultured cerebellar neurons were characterized by whole-cell electrophysiological studies and single cell PCR-based quantitation of subunit mRNA expression. Purkinje consistently expressed high levels Glu receptor 2 (GluR2) with low but nonzero Ca 2+ permeability. Other a wide range permeability fractional GluR2. These properties correlated on cell-by-cell basis. Their relationship was well fit model that assumed stochastic assembly subunits GluR2 dominance controlling...
The signature events caused by host-guest interactions in the nanopore system can be used as a novel and characteristic signal quantitative detection analysis of various molecules. However, effect several electrochemical factors on still remains unknown. Here, we systematically studied interactions, especially oscillation DNA-azide adamantane@cucurbit[6] α-Hemolysin under varying pH, concentration electrolytes counterions (Li+, Na+, K+). Our results indicate correlations between change pH...
Pneumococcal meningitis (PM), caused by Streptococcus pneumonia, remains a high-burden disease in developing countries. Antibiotic therapy has been limited due to the inefficiency of drug transport across blood-brain barrier (BBB) and emergence drug-resistant strains. In our preliminary study, PEGylated nano-self-assemblies bacitracin A (PEGylated Nano-BA12K) demonstrated strong antibacterial potency against S. pneumonia. this potential application micelle for treatment both...
The current study focused on the extraction technology, components analysis, in vitro antioxidant and antibacterial activities of total flavonoids fatty acids from Tribulus terrestris L. fruits. process was optimized by response surface method, compositions were identified two extracts HPLC-DAD-ESI-MS- GC-MS, respectively. In addition, evaluated assay ABTS, DPPH radical scavenging activity, ferric reducing power minimal inhibitory concentration. yields 0.46 9.76% under conditions. Moreover,...
Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-associated death in United States and has a 5-year survival rate <4%. Although much effort been invested research development pancreatic cancer drugs over past 30 years, due to lack effective targetable carcinogenic drivers, no new targeted therapies that can improve patient prognosis have approved for clinical use. SHR-A1403 c-mesenchymal-epithelial transition factor (c-MET) antibody-drug conjugate be used...
Nanopore sensing of proteomic biomarkers lacks accuracy due to the ultralow abundance targets, a wide variety interferents in clinical samples, and mismatch between pore analyte sizes. By converting antigens DNA probes via click chemistry quantifying their characteristic signals, we show nanopore assay with several amplification mechanisms achieve an attomolar level limit detection that enables quantitation circulating Mycobacterium tuberculosis (Mtb) antigen ESAT-6/CFP-10 complex human...