A5040047875- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Crop Yield and Soil Fertility
- Chromosomal and Genetic Variations
- Genetics and Plant Breeding
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Ruminant Nutrition and Digestive Physiology
- Plant Reproductive Biology
- Soil Carbon and Nitrogen Dynamics
- Photosynthetic Processes and Mechanisms
- Genetic and phenotypic traits in livestock
- Soil Mechanics and Vehicle Dynamics
- Agronomic Practices and Intercropping Systems
- Wheat and Barley Genetics and Pathology
- Mineral Processing and Grinding
- Agricultural Productivity and Crop Improvement
- Genomics and Chromatin Dynamics
- Plant Gene Expression Analysis
- GABA and Rice Research
- Plant tissue culture and regeneration
- Agricultural Engineering and Mechanization
China Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Shanghai CASB Biotechnology (China)
2024
Sanya University
2024
Hebei Academy of Agriculture and Forestry Sciences
2011-2022
Hengshui University
2022
Fudan University Shanghai Cancer Center
2019
Shanghai Medical College of Fudan University
2019
Academy of Agricultural and Forestry Sciences
2015
Cotton Research Institute
2013
Abstract Maize is an important crop with a high level of genome diversity and heterosis. The sequence typical female line, B73, was previously released. Here, we report de novo assembly corresponding male representative Mo17. More than 96.4% the 2,183 Mb assembled can be accounted for by 362 scaffolds in ten pseudochromosomes 38,620 annotated protein-coding genes. Comparative analysis revealed large gene-order gene structural variations: approximately 10% genes were mutually nonsyntenic,...
Summary Plants can respond to environmental changes with various mechanisms occurred at transcriptional and translational levels. Thus far, there have been relatively extensive understandings of stress responses plants on level, while little information is known about that level. To uncover the landscape translation in response drought stress, we performed recently developed ribosome profiling assay maize seedlings growing under normal conditions. Comparative analysis data RNA ‐seq showed...
Drought is one of the major factors limiting global maize production. Exposure to long-term drought conditions inhibits growth and leads yield losses. Although several drought-responsive genes have been identified functionally analyzed, mechanisms underlying responses water recovery treatments not fully elucidated. To characterize how seedling respond stress at transcriptional level, we analyzed physiological differentially expressed (DEGs) in inbred line B73 under deficit conditions.The...
The early maize (Zea mays) seed undergoes several developmental stages after double fertilization to become fully differentiated within a short period of time, but the genetic control this highly dynamic and complex process remains largely unknown. Here, we report high temporal-resolution investigation transcriptomes using 31 samples collected at an interval 4 or 6 h first six days development. These time-course were clearly separated into four distinct groups corresponding fertilization,...
Abstract A complete telomere-to-telomere (T2T) finished genome has been the long pursuit of genomic research. Through generating deep coverage ultralong Oxford Nanopore Technology (ONT) and PacBio HiFi reads, we report here a assembly maize with each chromosome entirely traversed in single contig. The 2,178.6 Mb T2T Mo17 base accuracy over 99.99% unveiled structural features all repetitive regions genome. There were several super-long simple-sequence-repeat arrays having consecutive...
Although genetic imprinting was discovered in maize 40 years ago, its exact extent the triploid endosperm remains unknown. Here, we have analyzed global patterns of allelic gene expression developing endosperms from reciprocal crosses between inbreds B73 and Mo17. We defined an imprinted as one which relative maternal paternal alleles differ at least fivefold both hybrids crosses. found that 179 genes (1.6% protein-coding genes) expressed are imprinted, with 68 them showing preferential 111...
Abstract Broomcorn millet ( Panicum miliaceum L.) has strong tolerance to abiotic stresses, and is probably one of the oldest crops, with its earliest cultivation that dated back ca . ~10,000 years. We report here genome assembly through a combination PacBio sequencing, BioNano, Hi-C (in vivo) mapping. The 18 super scaffolds cover ~95.6% estimated (~887.8 Mb). There are 63,671 protein-coding genes annotated in this tetraploid genome. About ~86.2% syntenic foxtail have two homologous copies...
Summary Plant RNA virus‐based guide (gRNA) delivery has substantial advantages compared to that of the conventional constitutive promoter‐driven expression due rapid and robust amplification gRNAs during virus replication movement. To date, virus‐induced genome editing tools have not been developed for wheat maize. In this study, we engineered a barley stripe mosaic (BSMV)‐based gRNA system clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9‐mediated targeted mutagenesis...
Abstract Long-range chromatin interactions are important for transcriptional regulation of genes, many which related to complex agronomics traits. However, the pattern three-dimensional remains unclear in plants. Here we report generation interaction analysis by paired-end tag sequencing (ChIA-PET) data and construction extensive H3K4me3- H3K27ac-centered maps maize. Results show that interacting patterns between proximal distal regulatory regions genes highly dynamic. Genes with have higher...
Doubled haploid technology has been widely applied to multiple plant species and is recognized as one of the most important technologies for improving crop breeding efficiency. Although mutations in MATRILINEAL/Zea mays PHOSPHOLIPASE A1/NOT LIKE DAD (MTL/ZmPLA1/NLD) Zea DOMAIN OF UNKNOWN FUNCTION 679 MEMBRANE PROTEIN (ZmDMP) have shown generate haploids maize, knowledge genetic basis induction (HI) remains incomplete. Therefore, cloning new genes underlying HI further elucidating its...
VQ motif-containing proteins play crucial roles in abiotic stress responses plants. Recent studies have shown that some physically interact with WRKY transcription factors to activate downstream genes. In the present study, we identified and characterized genes encoding using most recent version of maize genome sequence. total, 61VQ were identified. a cluster analysis, these clustered into nine groups together their homologous rice Arabidopsis. Most (57 out 61 numbers) found be single-copy...
Maternal-to-filial nutrition transfer is central to grain development and yield. nitrate transporter 1/peptide (NRT1-PTR)-type transporters typically transport nitrate, peptides, ions. Here, we report the identification of a maize (Zea mays) NRT1-PTR-type that transports sucrose glucose. The activity this sugar transporter, named Sucrose Glucose Carrier 1 (SUGCAR1), was systematically verified by tracer-labeled uptake serial electrophysiological studies including two-electrode voltage-clamp,...
Agronomic and quality traits in alfalfa are very important to forage industry. Genomic prediction based on genotyping-by-sequencing (GBS) data could shorten the breeding cycles accelerate genetic gains of these complex traits, if they display moderate high accuracies. The aim this study was investigate predictive potentials alfalfa. A total 322 genotypes from 75 accessions were used for genomic agronomic which related yield nutrition value, respectively, using BayesA, BayesB, BayesCπ...
RNA editing is a posttranscriptional process that important in mitochondria and plastids of higher plants. All editing-specific trans-factors reported so far belong to PLS-class pentatricopeptide repeat (PPR) proteins. Here, we report the map-based cloning molecular characterization defective kernel mutant dek39 maize. Loss Dek39 function leads delayed embryogenesis endosperm development, reduced size, seedling lethality. encodes an E sub-class PPR protein targets both chloroplasts, involved...
A CRISPR/Cas12i.3-based gene editing platform is established in broomcorn millet (Panicum miliaceum) and used to create new elite germplasm for this ancient crop.
This report presents Wan, a comprehensive and open suite of video foundation models designed to push the boundaries generation. Built upon mainstream diffusion transformer paradigm, Wan achieves significant advancements in generative capabilities through series innovations, including our novel VAE, scalable pre-training strategies, large-scale data curation, automated evaluation metrics. These contributions collectively enhance model's performance versatility. Specifically, is characterized...
The CRISPR-Cas systems have been widely used as genome editing tools, with type II and V typically introducing small indels, I system mediating long-range deletions. However, the precision of for large fragment deletion is still remained to be optimized. Here, we developed a compact Cascade-Cas3 Dvu I-C Cas11c plant editing. was efficient introduce controllable up at least 20 kb using paired crRNAs. paired-crRNAs design also improved controllability deletions I-E system. sensitive spacer...
China is the second-largest maize producer and consumer globally. During production, Fusarium spp. often gets infected, mycotoxins like fumonisin contaminate it. Fumonisin has become most widely polluted mycotoxin type in China. Planting genetically - modified an economical effective approach to reducing pollution products. This study aimed evaluate effectiveness of two transgenic events from China, Bt-Cry1Ab-Ma CM8101 Bt-Cry1Ab, Cry2Ab, G10evo Ruifeng 8, under stress natural Lepidopteran...