A5020754630- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Light effects on plants
- Agronomic Practices and Intercropping Systems
- CRISPR and Genetic Engineering
- Plant Stress Responses and Tolerance
- Soybean genetics and cultivation
- Plant Gene Expression Analysis
- Pluripotent Stem Cells Research
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Genomics and Chromatin Dynamics
- Plant Physiology and Cultivation Studies
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Nematode management and characterization studies
- Flowering Plant Growth and Cultivation
- Seed Germination and Physiology
Wageningen University & Research
2014-2024
Graduate School Experimental Plant Sciences
2012-2022
Utrecht University
2009-2018
Cancer Genomics Centre
2015
Universidad de Extremadura
2012
University of Tübingen
2006
Centre for BioSystems Genomics
2005
Stem cells self-renew and produce daughter that differentiate. How stem are specified maintained is a central question in developmental biology. Plant occupy small region or niche larger zones of mitotic activity called meristems. Here we provide molecular evidence the Arabidopsis root meristem, cell population depends on group cells, quiescent center (QC), which positions niche. We show putative transcription factor SCARECROW (SCR), first identified by its role radial patterning, required...
Plants and some animals have a profound capacity to regenerate organs from adult tissues. Molecular mechanisms for regeneration have, however, been largely unexplored. Here we investigate local response in Arabidopsis roots. Laser-induced wounding disrupts the flow of auxin-a cell-fate-instructive plant hormone-in root tips, demonstrate that resulting cell-fate changes require PLETHORA, SHORTROOT, SCARECROW transcription factors. These factors regulate expression polar position PIN auxin...
Significance Fixed nitrogen is essential for plant growth. Some plants, such as legumes, can host nitrogen-fixing bacteria within cells in root organs called nodules. Nodules are considered to have evolved parallel different lineages, but the genetic changes underlying this evolution remain unknown. Based on gene expression nonlegume Parasponia andersonii and legume Medicago truncatula , we find that nodules these lineages may share a single origin. Comparison of genomes with those related...
In the Arabidopsis root meristem, ground tissue stem cell daughters perform an asymmetric division to form endodermis and cortex. The putative transcription factors SCARECROW (SCR) SHORTROOT (SHR) regulate this radial patterning event, mixed fate in scr mutants suggests atypical role of SCR gene division. Here we use a newly developed site-specific activation/deletion system which induced clones are positively marked with green fluorescent protein (GFP). Using system, show that acts...
Abstract CLAVATA3 (CLV3), CLV3/ESR19 (CLE19), and CLE40 belong to a family of 26 genes in Arabidopsis thaliana that encode putative peptide ligands with unknown identity. It has been shown previously ectopic expression any these three leads consumption the root meristem. Here, we show vitro application synthetic 14–amino acid peptides, CLV3p, CLE19p, CLE40p, corresponding conserved CLE motif, mimics overexpression phenotype. The same result was observed when CLE19 protein applied externally....
In the Arabidopsis root, SHORT-ROOT transcription factor moves outward to ground tissue from its site of in stele and is required for specification endodermis stem cell organizing quiescent center cells. addition, downstream SCARECROW control an oriented division daughters. Here, we show that JACKDAW MAGPIE genes, which encode members a plant-specific family zinc finger proteins, act SHR-dependent feed-forward loop regulate range action SCARECROW. expression initiated independent regulates...
We used a semiquantitative root hair deformation assay for Vicia sativa (vetch) to study the activity of Rhizobium leguminosarum bv viciae nodulation (Nod) factors. Five 10 min Nod factor-root interaction appears be sufficient induce deformation. The first is visible within 1 h, and after 3 h about 80% hairs in small susceptible zone are deformed. This encompasses that have almost reached their maximal size. factor accumulates preferentially epidermal cells young part root, but not...
ABSTRACT Nod factors secreted by Rhizobium leguminosarum bv. viciae induce root hair deformation, involving a reinitiation of tip growth, and the formation nodule primordia in Vicia sativa (vetch). Ethylene is potent inhibitor cortical cell division, an effect that can be counteracted applying silver ions (Ag+) or aminoethoxy-vinylglycine (AVG). In contrast to inhibitory on ethylene promotes hairs (which involves growth) epidermis Arabidopsis. We investigate possible paradox concerning...
At a nonpermissive temperature, somatic embryos of the temperature-sensitive (ts) carrot cell mutant ts11 only proceed beyond globular embryo stage in presence medium conditioned by wild-type embryos. The causative component has previously been identified as 32-kD acidic endochitinase. In search function for this enzyme plant embryogenesis, several compounds that contain oligomers N-acetylglucosamine were tested their ability to promote formation. Of these compounds, Rhizobium...
Organ formation in animals and plants relies on precise control of cell state transitions to turn stem daughters into fully differentiated cells. In plants, cells cannot rearrange due shared walls. Thus, differentiation progression the accompanying expansion must be tightly coordinated across tissues. PLETHORA (PLT) transcription factor gradients are unique their ability guide at different positions growing Arabidopsis thaliana root, which contrasts with well-described specifying distinct...
A powerful method to study gene function is expression or overexpression in an inducible, cell type-specific system followed by observation of consequent phenotypic changes and visualization linked reporters the target tissue. Multiple inducible systems have been developed for plants, but very few these combine plant selection markers, control domains, access multiple promoters protein fusion reporters, chemical induction, high-throughput cloning capabilities. Here, we introduce a MultiSite...
Roots are important to plants for a wide variety of processes, including nutrient and water uptake, anchoring mechanical support, storage functions, as the major interface between plant various biotic abiotic factors in soil environment. Therefore, understanding development architecture roots holds potential manipulation root traits improve productivity sustainability agricultural systems better understand manage natural ecosystems. While lateral is traceable process along primary different...
Continuous formation of somatic tissues in plants requires functional stem cell niches where undifferentiated cells are maintained. In Arabidopsis thaliana , PLETHORA ( PLT ) and SCARECROW SCR genes outputs apical–basal radial patterning systems, both required for root specification maintenance. The WUSCHEL-RELATED HOMEOBOX 5 WOX5 gene is specifically expressed functions a small group organizer cells, also called the quiescent center (QC). QC function, their expression overlaps QC; however,...