A5058758292- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Data Quality and Management
- Polysaccharides and Plant Cell Walls
- Light effects on plants
- Data Mining Algorithms and Applications
- Advanced Database Systems and Queries
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Software System Performance and Reliability
- Privacy-Preserving Technologies in Data
- Cloud Data Security Solutions
- Service-Oriented Architecture and Web Services
- Plant Taxonomy and Phylogenetics
- Data Management and Algorithms
- Web Data Mining and Analysis
- Plant Parasitism and Resistance
- Plant and Biological Electrophysiology Studies
- Plant Diversity and Evolution
- Plant Disease Resistance and Genetics
- Connective tissue disorders research
- Recreation, Leisure, Wilderness Management
- Software Engineering Research
Indian Institute of Science Education and Research Pune
2021-2024
Indian Institute of Science Education and Research Thiruvananthapuram
2014-2023
The University of Sydney
2021
Indian Institute of Science Education and Research Berhampur
2019
Indian Institute of Science Education and Research Mohali
2019
Indian Institute of Science Education and Research Kolkata
2019
Indian Institute of Science Education and Research, Bhopal
2019
Indian Institute of Science Education and Research, Tirupati
2019
Utrecht University
2007-2016
IBM Research - India
2009-2013
Polar membrane cargo delivery is crucial for establishing cell polarity and directional transport processes. In plants, polar trafficking mediates the dynamic asymmetric distribution of PIN FORMED (PIN) carriers, which drive cell-to-cell hormone auxin, thereby generating auxin maxima minima that control development. The Arabidopsis PINOID (PID) protein kinase instructs apical localization by phosphorylating PINs. Here, we identified PID homologs WAG1 WAG2 as new regulators. We show AGC3...
Summary Grass flowers are highly derived compared to their eudicot counterparts. To delineate OsMADS1 functions in rice floret organ development we have examined its evolution and the consequences of knockdown or overexpression. Molecular phylogeny suggests co‐evolution with grass family diversification. perturbs differentiation specific cell types lemma palea, creating glume‐like features, severe derangements differentiation. Conversely, ectopic expression suffices direct lemma‐like glume....
Activity of axillary meristems dictates the architecture both vegetative and reproductive parts a plant. In Arabidopsis thaliana , model eudicot species, transcription factor LFY confers floral fate to new arising from periphery shoot apex. Diverse orthologous genes regulate vegetative-to-reproductive phase transition when expressed in property not shared by RFL homolog agronomically important grass, rice. We have characterized knockdown its expression ectopic overexpression transgenic find...
Organ formation in animals and plants relies on precise control of cell state transitions to turn stem daughters into fully differentiated cells. In plants, cells cannot rearrange due shared walls. Thus, differentiation progression the accompanying expansion must be tightly coordinated across tissues. PLETHORA (PLT) transcription factor gradients are unique their ability guide at different positions growing Arabidopsis thaliana root, which contrasts with well-described specifying distinct...
Lateral organ distribution at the shoot apical meristem defines specific and robust phyllotaxis patterns that have intrigued biologists mathematicians for centuries. In silico studies revealed this self-organizing process can be recapitulated by modeling polar transport of phytohormone auxin. Phyllotactic change between species developmental stages, but processes behind these variations remained unknown. Here we use regional complementation experiments to reveal phyllotactic switches in...
A powerful method to study gene function is expression or overexpression in an inducible, cell type-specific system followed by observation of consequent phenotypic changes and visualization linked reporters the target tissue. Multiple inducible systems have been developed for plants, but very few these combine plant selection markers, control domains, access multiple promoters protein fusion reporters, chemical induction, high-throughput cloning capabilities. Here, we introduce a MultiSite...
De novo shoot regeneration in tissue culture undergoes at least two phases. Explants are first cultured on auxin-rich callus-inducing medium (CIM) to produce a group of pluripotent cells termed callus; the callus is then transferred cytokinin rich shoot-inducing (SIM) promote formation progenitor cells, from which adventitious shoots may differentiate. Here, we show that Arabidopsis thaliana transcription factor gene LATERAL ORGAN BOUNDARIES DOMAIN16 (LBD16) involved pluripotency acquisition...
Abstract Functional diversification of duplicated genes can contribute to the emergence new organ morphologies. Model eudicot plants like Arabidopsis thaliana and Antirrhinum majus have a single PI/GLO gene that together with AP3/DEF regulate petal stamen formation. Lodicules grass flowers are morphologically distinct reduced organs occupying position petals in other flowers. They serve function partial transient flower opening allow cross-pollination. Grasses PI/GLO-like rice (Oryza sativa)...
The MADS-box family of transcription factors have diverse developmental roles in flower pattern formation, gametophyte cell division and fruit differentiation. B-sister proteins are most similar to the B-class floral homeotic proteins, expressed female reproductive organs. Arabidopsis protein, TT16, is necessary for inner integument We functionally characterized only other gene Arabidopsis, AGL63, renamed here as GORDITA (GOA). A loss-of-function mutation goa or reduction endogenous GOA...
A wide variety of multicellular organisms across the kingdoms display remarkable ability to restore their tissues or organs when they suffer damage. However, repair damage is not uniformly distributed throughout body parts. Here, we unravel elusive mechanistic basis boundaries on organ regeneration potential using root tip resection as a model and show that dosage gradient-expressed PLT2 transcription factor underlying cause. While transient downregulation in distinct set plt mutant...
Unlike many eudicot species, grasses have duplicated PI/GLO-like genes. Functional analysis of one the rice PI/GLO paralogs, OsMADS2, is reported here. Our data demonstrate its essential role in lodicule development and implicate second paralog, OsMADS4, to suffice for stamen specification. We provide first evidence differential contributions grass paralogs patterning second- third-whorl floral organs.
Despite their pivotal role in plant development, control mechanisms for oriented cell divisions have remained elusive. Here, we describe how a precisely regulated division orientation switch an Arabidopsis stem is controlled by upstream patterning factors. We show that the regulatory PLETHORA transcription factors induce plane reorientation local activation of auxin signaling, culminating enhanced expression microtubule-associated MAP65 proteins. upregulation sufficient to reorient cortical...