A5048037808- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Hippo pathway signaling and YAP/TAZ
- Circadian rhythm and melatonin
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Lipid metabolism and biosynthesis
- Tree Root and Stability Studies
- Biotin and Related Studies
- Kruppel-like factors research
- 3D Printing in Biomedical Research
- Polysaccharides and Plant Cell Walls
- DNA Repair Mechanisms
- Gene Regulatory Network Analysis
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Spaceflight effects on biology
- Genetic Mapping and Diversity in Plants and Animals
- Light effects on plants
- Plant Stress Responses and Tolerance
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
Utrecht University
2015-2024
Wageningen University & Research
2020-2022
RWTH Aachen University
2020
Premitotic control of cell division orientation is critical for plant development, as walls prevent extensive remodeling or migration. While many divisions are proliferative and add cells to existing tissues, some formative generate new tissue layers growth axes. Such often asymmetric in nature, producing daughters with different fates. We have previously shown that, the Arabidopsis thaliana embryo, developmental asymmetry correlated geometric asymmetry, creating daughter unequal volume....
Affinity purification followed by mass spectrometry (AP/MS) is a widely used approach to identify protein interactions and complexes. In multicellular organisms, the accurate identification of complexes AP/MS complicated potential heterogeneity in different tissues. Here, we present an vivo biotinylation-based for tissue-specific from Caenorhabditis elegans. Tissue-specific biotinylation achieved expression select tissues bacterial biotin ligase BirA, which biotinylates proteins tagged with...
ABSTRACT ERM proteins are conserved regulators of cortical membrane specialization that function as membrane-actin linkers and molecular hubs. The activity requires a conformational switch from an inactive cytoplasmic form into active membrane- actin-bound form, which is thought to be mediated by sequential PIP2 binding phosphorylation C-terminal threonine residue. Here, we use the single Caenorhabditis elegans ortholog, ERM-1, study contribution these regulatory events tissue formation in...
The eukaryotic genome is organized in a three-dimensional structure called chromatin, constituted by DNA and associated proteins, the majority of which are histones. Post-translational modifications histone proteins greatly influence chromatin regulate many DNA-based biological processes. Methylation lysine 36 3 (H3K36) post-translational modification functionally relevant during early steps damage repair. Here, we show that JMJD-5 regulates H3K36 di-methylation it required at late stages...
ABSTRACT Crumbs proteins are important regulators of epithelial polarity. In C. elegans, no essential role for the two described homologs has been uncovered. Here, we identify and characterize an additional family member in which termed CRB-3 based on its similarity size sequence to mammalian CRB3. We visualized subcellular localization by expressing a translational GFP fusion. CRB-3::GFP was expressed several polarized tissues embryo larval stages, showed apical intestine pharynx. To...
Epithelial tubes are essential components of metazoan organ systems that control the flow fluids and exchange materials between body compartments outside environment. The size shape central lumen confer important characteristics to tubular organs need be carefully controlled. Here, we identify small coiled-coil protein BBLN-1 as a regulator morphology in C. elegans intestine. Loss causes formation bubble-shaped invaginations apical membrane into cytoplasm intestinal cells abnormal...
Reorganization of the plasma membrane and underlying actin cytoskeleton into specialized domains is essential for functioning most polarized cells in animals. Proteins ezrin-radixin-moesin (ERM) Na+/H+ exchanger 3 regulating factor (NHERF) family are conserved regulators cortical specialization. ERM proteins function as membrane-actin linkers molecular scaffolds that organize distribution at membrane. NHERF PDZ-domain containing adapters can bind to extend their scaffolding capability. Here,...
Crumbs proteins are evolutionarily conserved transmembrane with essential roles in promoting the formation of apical domain epithelial cells. The short intracellular tail known to interact several proteins, including scaffolding protein PALS1 (protein associated LIN7, Stardust Drosophila). turn binds a second PATJ (PALS1-associated tight junction protein) form core Crumbs/PALS1/PATJ complex. While organization have been shown for Drosophila and mammalian systems, three Caenorhabditis elegans...
The invariant lineages of C. elegans provide tractable cell fate models to study how developing organisms robustly integrate spatial signals at the single-cell level via gene regulatory networks. For instance, during postembryonic development, a mesoderm lineage arises through sequence oriented divisions from single progenitor. This mesoblast initially gives rise 18 cells with three distinct fates – 14 body wall muscles (BWMs), 2 coelomocytes (CCs; dorsal), and sex myoblasts (SMs; ventral)....
Abstract Epithelial tubes are essential components of metazoan organ systems that control the flow fluids and exchange materials between body compartments outside environment. The size shape central lumen confer important characteristics to tubular organs need be carefully controlled. Here, we identify small coiled-coil protein BBLN-1 as a regulator morphology in C. elegans intestine. Loss causes formation bubble-shaped invaginations apical membrane into cytoplasm intestinal cells, abnormal...
Abstract ERM proteins are conserved regulators of cortical membrane specialization, that function as membrane–actin linkers and molecular hubs. Activity requires a conformational switch from an inactive cytoplasmic form into active membrane- actin-bound form, which is thought to be mediated by sequential PIP 2 -binding phosphorylation C-terminal threonine residue. Here, we use the single C. elegans ortholog, ERM-1, study contribution these regulatory events activity tissue formation in vivo...
Abstract The apical domain of epithelial cells can acquire a diverse array morphologies and functions, which is critical for the function tissues. Crumbs proteins are evolutionary conserved transmembrane with essential roles in promoting formation cells. short intracellular tail interacts variety proteins, including scaffolding protein Pals1 (protein associated LIN7, Stardust Drosophila ). turn binds to second termed PATJ (Pals1-associated tight junction protein), form core Crumbs/Pals1/PATJ...
Abstract Reorganization of the plasma membrane and underlying actin cytoskeleton into specialized domains is essential for functioning most polarized cells in animals. Proteins ezrin-radixin-moesin (ERM) Na + /H exchanger 3 regulating factor (NHERF) family are conserved regulators cortical specialization. ERM proteins function as membrane-actin linkers molecular scaffolds that organize distribution at membrane. NHERF PDZ-domain containing adapters can bind to extend their scaffolding...