A5087100106- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant and animal studies
- Plant Physiology and Cultivation Studies
- Plant Pathogens and Fungal Diseases
- Wheat and Barley Genetics and Pathology
- Child Nutrition and Water Access
- Plant Virus Research Studies
- Plant Stress Responses and Tolerance
- Plant Water Relations and Carbon Dynamics
- Seed Germination and Physiology
- Plant Genetic and Mutation Studies
- Water Governance and Infrastructure
- Transboundary Water Resource Management
- Microbial Metabolic Engineering and Bioproduction
- Human Rights and Development
- Bioenergy crop production and management
- Chromosomal and Genetic Variations
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
- Plant Taxonomy and Phylogenetics
- Genetic Mapping and Diversity in Plants and Animals
- International Development and Aid
University of Nottingham
2014-2023
Loughborough University
2009-2021
University of KwaZulu-Natal
2004-2019
Massachusetts Institute of Technology
2019
Institute of Crop Science
2015
Shanghai Jiao Tong University
2008-2012
Center for Excellence in Molecular Plant Sciences
2008
China National Rice Research Institute
2008
Chinese Academy of Sciences
2008
Africa Society for Blood Transfusion
2006
The Arabidopsis thaliana ABORTED MICROSPORES (AMS) gene encodes a basic helix-loop-helix (bHLH) transcription factor that is required for tapetal cell development and postmeiotic microspore formation. However, the regulatory role of AMS in anther pollen has not been fully defined. Here, we show by microarray analysis expression 549 anther-expressed genes was altered ams buds these are associated with function wall We demonstrate ability to bind vitro DNA containing 6-bp consensus motif,...
Abstract The Arabidopsis thaliana MYB26/MALE STERILE35 (MS35) gene is critical for the development of secondary thickening in anther endothecium and subsequent dehiscence. MYB26 localized to nucleus regulates endothecial a cell-specific manner anther. expression seen anthers also style nectaries, although there no effect on female fertility ms35 mutant. occurs early during development, with maximal pollen mitosis I bicellular stages, indicating regulatory role specifying cell development....
Abstract In higher plants, timely degradation of tapetal cells, the innermost sporophytic cells anther wall layer, is a prerequisite for development viable pollen grains. However, relatively little known about mechanism underlying programmed cell and degradation. Here, we report key regulator in monocot rice (Oryza sativa), PERSISTANT TAPETAL CELL1 (PTC1), which controls functional formation. The evolutionary significance PTC1 was revealed by partial genetic complementation homologous...
Mature pollen is covered by durable cell walls, principally composed of sporopollenin, an evolutionary conserved, highly resilient, but not fully characterized, biopolymer aliphatic and aromatic components. Here, we report that ABORTED MICROSPORES (AMS) acts as a master regulator coordinating wall development sporopollenin biosynthesis in Arabidopsis thaliana. Genome-wide coexpression analysis revealed 98 candidate genes with specific expression the anther 70 showed reduced ams. Among these...
Summary The Arabidopsis thaliana MALE STERILITY 2 ( MS2 ) gene product is involved in male gametogenesis. first abnormalities pollen development of ms2 mutants are seen at the stage microsporogenesis when microspores released from tetrads. Expression observed tapetum wild‐type flowers at, and shortly after, release promoter controls GUS expression a comparable transgenic tobacco containing an promoter‐GUS fusion. occasional grains produced by mutant plants have very thin walls. They also...
Summary We report here the molecular characterisation of Arabidopsis MALE STERILITY1 gene, which is a critical sporophytic controlling factor for anther and pollen development. Homozygous ms1 mutants do not produce viable pollen, but are otherwise phenotypically normal. Degeneration occurs soon after microspore release from tetrads, at time tapetum also appears abnormally vacuolated. The MS1 gene expressed low levels in anthers closed buds, with expression stage release. No seen open...
Abstract The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation and has homology to the PHD-finger class of transcription factors; however, its role in development not been fully defined. We show that MS1 appears be autoregulated by wild-type transcript or protein. Using a functional green fluorescent protein (GFP) fusion analyze temporal spatial expression MS1, we demonstrate MS1:GFP nuclear localized within tapetum expressed developmentally regulated...
Summary A male sterile mutant with a defect in anther dehiscence was identified an Arabidopsis thaliana population mutagenized the Zea mays transposon En‐1/Spm. Mutants produce viable pollen that can fertilize when released mechanically from anthers. Mutant stamens are of normal size and shape, but lack cell wall fortifications endothecial layer anther, which required for process. The phenotype shown to be caused by insertion AtMYB26 , disrupting putative DNA‐binding domain this R2R3‐type...
Gibberellin (GA) biosynthesis is necessary for normal plant development, with later GA biosynthetic stages being governed by multigene families. Arabidopsis thaliana contains five 20-oxidase (GA20ox) genes, and past work has demonstrated the importance of GA20ox1 -2 growth fertility. Here, we show through systematic mutant analysis that GA20ox1, -2, -3 are dominant paralogs; their absence results in severe dwarfism almost complete loss In vitro revealed GA20ox4 full GA20ox activity, but...
Abstract Poaceae, one of the largest flowering plant families in angiosperms, evolved distinct inflorescence and flower morphology diverging from eudicots other monocots. However, mechanism underlying specification grasses remains unclear. Here we show that floral zygomorphy along lemma-palea axis rice (Oryza sativa) is partially or indirectly determined by CYCLOIDEA (CYC)-like homolog RETARDED PALEA1 (REP1), which regulates palea identity development. The REP1 gene only expressed primordium...
Summary Viable pollen is essential for plant reproduction and crop yield. Its production requires coordinated expression at specific stages during anther development, involving early meiosis‐associated events late wall formation. The ABORTED MICROSPORE S ( AMS ) transcription factor a master regulator of sporopollenin biosynthesis, secretion formation in Arabidopsis . Here we show that it has complex regulation additional roles earlier An inducible‐ reporter was created functional rescue,...
The Arabidopsis thaliana MALE STERILITY 2 ( MS2 ) gene product is involved in male gametogenesis. first abnormalities pollen development of ms2 mutants are seen at the stage microsporogenesis when microspores released from tetrads. Expression observed tapetum wild‐type flowers at, and shortly after, release promoter controls GUS expression a comparable transgenic tobacco containing an promoter–GUS fusion. occasional grains produced by mutant plants have very thin walls. They also sensitive...
Background SET-domain proteins are histone lysine (K) methyltransferases (HMTase) implicated in defining transcriptionally permissive or repressive chromatin. The Arabidopsis ASH1 HOMOLOG 2 (ASHH2) protein (also called SDG8, EFS and CCR1) has been suggested to methylate H3K4 and/or H3K36 is similar Drosophila ASH1, a positive maintainer of gene expression, yeast Set2, HMTase. Mutation the ASHH2 pleiotropic developmental effects. Here we focus on role plant reproduction. Methodology/Principal...
Soybean [Glycine max (L.) Merr.] is one of the world's major crops, and soybean seeds are a rich important resource for proteins oils. While "omics" studies, such as genomics, transcriptomics, proteomics, have been widely applied in molecular research, fewer metabolomic studies conducted large-scale detection low weight metabolites, especially seeds. In this study, we investigated seed metabolomes 29 common cultivars through combined gas chromatography-mass spectrometry ultra-performance...
Summary Excessive gibberellin ( GA ) signalling, mediated through the DELLA proteins, has a negative impact on plant fertility. Loss of activity in monocot rice Oryza sativa causes complete male sterility, but not dicot model Arabidopsis thaliana ecotype Landsberg erecta (L er ), which function been studied most extensively, leading to assumption that is essential for pollen development. A novel fertility phenotype was identified Columbia (Col‐0) necessitates re‐evaluation general...
Successful fertilization relies on the production and effective release of viable pollen. Failure anther opening (dehiscence), results in male sterility, although pollen may be fully functional. MYB26 regulates formation secondary thickening endothecium, which is critical for dehiscence fertility. Here, we show that transcript shows expression multiple floral organs, protein localized specifically to endothecium nuclei it directly two NAC domain genes, NST1 NST2, are induction biosynthesis...
Abstract Sporophytic pollen coat proteins (sPCPs) derived from the anther tapetum are deposited into wall cavities and function in pollen–stigma interactions, hydration, environmental protection. In Arabidopsis, 13 highly abundant have been identified coat, including seven major glycine-rich GRP14, 16, 17, 18, 19, 20, GRP–oleosin; two caleosin-related family (AT1G23240 AT1G23250); three lipase EXL4, EXL5 EXL6, ATA27/BGLU20. Here, we show that EXL4 EXL6 fused with green fluorescent protein...
A key target for the improvement of Oryza sativa (rice) is development heat-tolerant varieties. This necessitates high-throughput methodologies screening heat tolerance. Progress has been made to this end via visual scoring and chlorophyll fluorescence; however, these approaches demand large infrastructural investments expose populations adult plants stress. To address bottleneck, we investigated response maximum quantum efficiency photosystem II (PSII) rapidly increasing temperatures in...